FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008142964

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008142964
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12782
Total Bases1.8 Mbp
Sequences flagged as poor quality0
Sequence length139-145
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAACAGGTCAGATGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGC644450.41464559536849No Hit
AGCACAGGACTCTTCTTTGGTATAAAGAGAGTACTTTTAAAGGTCCTGCT382829.948364888123923No Hit
ACAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGC8116.34485995931779No Hit
CGGAAACAACAACTGCTGAGAAGAGGAAGCTGTTCAGACCAAGTCTCTGC950.7432326709435143No Hit
ACACAGGTCAGATGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGCT690.5398216241589735No Hit
CAACAGGTCAGATGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGCT590.4615866061649194No Hit
GCACAGGACTCTTCTTTGGTATAAAGAGAGTACTTTTAAAGGTCCTGCTT470.36770458457205446No Hit
AAACAGGTCAGATGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGCT390.30511657017681115No Hit
ACAAAGGTCAGATGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGCT350.2738225629791895No Hit
ACAACAGGTCGATGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGCT300.23470505398216243No Hit
ACAACGGTCAGATGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGCT290.226881552182757No Hit
ACAACAGTCAGATGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGCT280.21905805038335158No Hit
AGACAGGACTCTTCTTTGGTATAAAGAGAGTACTTTTAAAGGTCCTGCTT230.1799405413863245No Hit
AGCACAGGATCTTCTTTGGTATAAAGAGAGTACTTTTAAAGGTCCTGCTT230.1799405413863245No Hit
AGCACAGGACTTTCTTTGGTATAAAGAGAGTACTTTTAAAGGTCCTGCTT200.1564700359881083No Hit
ACACAGGACTCTTCTTTGGTATAAAGAGAGTACTTTTAAAGGTCCTGCTT200.1564700359881083No Hit
ACAACAGGTAGATGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGCT190.14864653418870286No Hit
AGCAAGGACTCTTCTTTGGTATAAAGAGAGTACTTTTAAAGGTCCTGCTT180.14082303238929744No Hit
ACAACAGGTCAGATGCAGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGCT180.14082303238929744No Hit
ACAACAGGTCAGATGCAGGCCCAGCGGTGTCCCAAGCCCCCACCCAGCGC170.13299953058989203No Hit
ACAACAGGTCAGATGCAGGCCCAGCGGTGCCCCAAGCTCCCACCCAGCGC160.12517602879048662No Hit
ACAACAGGTCAGATGCAGGCCCAGCGGCGTCCCAAGCTCCCACCCAGCGC160.12517602879048662No Hit
ACAACAGGTCAATGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGCT160.12517602879048662No Hit
CGGAAACAACAACAGCTGAGAAGAGGAAGCTGTTCAGACCAAGTCTCTGC150.11735252699108122No Hit
ACAACAGGTCAGATGCGGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGC150.11735252699108122No Hit
ACAACAGGTCAGATGCAGGTCCAGCGGTGTCCCAAGCTCCCACCCAGCGC150.11735252699108122No Hit
ACAACAGGCAGATGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGCT150.11735252699108122No Hit
ACAACAGGTCAGATGCAGGCCCAGCAGTGTCCCAAGCTCCCACCCAGCGC150.11735252699108122No Hit
AGCACAGGACTCTTCTTTGGTATAAAGAGAGTACTTTTAAAGGCCCTGCT140.10952902519167579No Hit
ACAACAGGTCAGAGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGCT140.10952902519167579No Hit
ACAACAGGTCAGATGCAGGCCCAGCGGTGTCCCAAGTTCCCACCCAGCGC140.10952902519167579No Hit
AGCCAGGACTCTTCTTTGGTATAAAGAGAGTACTTTTAAAGGTCCTGCTT140.10952902519167579No Hit
ACAACAGGTCAGATCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGCT140.10952902519167579No Hit
ACAACAGGTCAGATGCAGGCCCGGCGGTGTCCCAAGCTCCCACCCAGCGC140.10952902519167579No Hit
AGCACAGGACTCTTCTTTGGTATAAAGAGGGTACTTTTAAAGGTCCTGCT130.10170552339227039No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)