FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008143004

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008143004
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18241
Total Bases2.6 Mbp
Sequences flagged as poor quality0
Sequence length142-145
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTAGTGACAAGAGGGACATCTCGCGATTTCTCGAGTCCAACCCTGTGA583631.993859985746397No Hit
ACAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGC495527.164080916616413No Hit
AGCACAGGACTCTTCTTTGGTATAAAGAGAGTACTTTTAAAGGTCCTGCT271014.8566416314895No Hit
ACAACAGGTCAGATGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGC220612.093635217367469No Hit
CGGAAACAACAACTGCTGAGAAGAGGAAGCTGTTCAGACCAAGTCTCTGC1640.8990735157063757No Hit
CGTTAGTGACAAGGGGGACATCTCGCGATTTCTCGAGTCCAACCCTGTGA600.3289293350145277No Hit
CGTTAGTGACAGAGGGACATCTCGCGATTTCTCGAGTCCAACCCTGTGAT500.2741077791787731No Hit
CGTTAGTGACAAGAGGGACATTTCGCGATTTCTCGAGTCCAACCCTGTGA480.2631434680116222No Hit
ACACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT480.2631434680116222No Hit
CAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT380.20832191217586754No Hit
AAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT360.19735760100871663No Hit
CGTTAGTGACAAGAGGGACATCTTGCGATTTCTCGAGTCCAACCCTGTGA340.1863932898415657No Hit
CGTAGTGACAAGAGGGACATCTCGCGATTTCTCGAGTCCAACCCTGTGAT340.1863932898415657No Hit
CGTTAGTGAAAGAGGGACATCTCGCGATTTCTCGAGTCCAACCCTGTGAT330.18091113425799024No Hit
ACAACAGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT300.16446466750726385No Hit
CAACAGGTCAGATGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGCT300.16446466750726385No Hit
CGTTAGTGACAAAGGGACATCTCGCGATTTCTCGAGTCCAACCCTGTGAT300.16446466750726385No Hit
ACAACGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT260.142536045172962No Hit
CGTTAGTGACAAGAGGGACTCTCGCGATTTCTCGAGTCCAACCCTGTGAT250.13705388958938655No Hit
ACAAAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT250.13705388958938655No Hit
CGTTAGTGCAAGAGGGACATCTCGCGATTTCTCGAGTCCAACCCTGTGAT250.13705388958938655No Hit
CGGAAACAACAACAGCTGAGAAGAGGAAGCTGTTCAGACCAAGTCTCTGC240.1315717340058111No Hit
GCACAGGACTCTTCTTTGGTATAAAGAGAGTACTTTTAAAGGTCCTGCTT240.1315717340058111No Hit
AGCAAGGACTCTTCTTTGGTATAAAGAGAGTACTTTTAAAGGTCCTGCTT210.1151252672550847No Hit
GTTAGTGACAAGAGGGACATCTCGCGATTTCTCGAGTCCAACCCTGTGAT200.10964311167150924No Hit
ACAACAGGTCAGATGCAGGCCCATCGGCGTCCCAAGCTCCCACCCAGCGC200.10964311167150924No Hit
CGTTATGACAAGAGGGACATCTCGCGATTTCTCGAGTCCAACCCTGTGAT190.10416095608793377No Hit
ACAACAGGTAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT190.10416095608793377No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)