FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008143022

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008143022
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12025
Total Bases1.7 Mbp
Sequences flagged as poor quality0
Sequence length142-145
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAACAGGTCAGATGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGC815567.81704781704782No Hit
AGCACAGGACTCTTCTTTGGTATAAAGAGAGTACTTTTAAAGGTCCTGCT211917.62162162162162No Hit
AGCACAGGACTCTTCTTTGGTATAAAGAGAGTACTTTTAAAGGTCTTGCT2001.6632016632016633No Hit
ACACAGGTCAGATGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGCT790.656964656964657No Hit
CAACAGGTCAGATGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGCT660.5488565488565489No Hit
ACAACGGTCAGATGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGCT480.3991683991683992No Hit
CGGAAACAACAACTGCTGAGAAGAGGAAGCTGTTCAGACCAAGTCTCTGC470.3908523908523909No Hit
AAACAGGTCAGATGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGCT420.3492723492723493No Hit
ACAAAGGTCAGATGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGCT390.3243243243243243No Hit
ACAACAGTCAGATGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGCT370.3076923076923077No Hit
ACAACAGGTCAGATGCAGGCCCAGCGGTGTCCCAAGCCCCCACCCAGCGC330.27442827442827444No Hit
ACAACAGGCAGATGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGCT300.2494802494802495No Hit
ACAACAGGTCGATGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGCT290.24116424116424115No Hit
ACAACAGGTCAATGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGCT250.2079002079002079No Hit
ACAACAGGTCAGATGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGTGC210.17463617463617465No Hit
GCACAGGACTCTTCTTTGGTATAAAGAGAGTACTTTTAAAGGTCCTGCTT210.17463617463617465No Hit
ACAACAGGTCAGATCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGCT190.158004158004158No Hit
ACAACAGGTCAGATGCGGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGC180.1496881496881497No Hit
ACAACAGGTCAGATGCAGGCCCAGCGGTGCCCCAAGCTCCCACCCAGCGC180.1496881496881497No Hit
ACAACAGGTCAGATGCAGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGCT170.14137214137214135No Hit
ACAACAGGTCAGATGCAGGTCCAGCGGTGTCCCAAGCTCCCACCCAGCGC170.14137214137214135No Hit
ACAACAGGTAGATGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGCT160.13305613305613306No Hit
ACAACAGGTCAGATGCAGGCCCAGCGGCGTCCCAAGCTCCCACCCAGCGC160.13305613305613306No Hit
ACAACAGGTCAGATGCAGGCCCAGCGGTGTCCCGAGCTCCCACCCAGCGC150.12474012474012475No Hit
ACAACAGGTCAGAGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGCT140.11642411642411642No Hit
ACAACAGGTCAGATGCGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGCT140.11642411642411642No Hit
ACAACAGGTCAGATGCAGGCCCAGCGGTGTCCCAAGCTCCCGCCCAGCGC140.11642411642411642No Hit
ACAACAGGTCAGATGCAGGCCCGGCGGTGTCCCAAGCTCCCACCCAGCGC140.11642411642411642No Hit
ACAACAGGTCAGTGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGCT130.10810810810810811No Hit
ACAACAGGTCAGATGCAGGCCCAGTGGTGTCCCAAGCTCCCACCCAGCGC130.10810810810810811No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)