FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008143039

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008143039
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31572
Total Bases4.5 Mbp
Sequences flagged as poor quality0
Sequence length139-145
%GC58

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTATCCTGCCATGCTCCAGACACTCCTGCAGCTCCAGCTTATCATTCAC1064433.71341695172938No Hit
AGACCCCTGGAACTGCTACATGTGCGGGCACAAGGGTACCTACGGGCTGC673321.325858355504877No Hit
CTTCCTCTTCTCAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCAAATT384912.191182060053212No Hit
TGGCTCATCTTCAAACCGTCTCCTGTTTTGTAGTCCAACCCTGTGATGAT325410.306600785506145No Hit
AGACCCCTGGAACTGCTACCTGTGCGGGCACAAGGGTACCTACGGGCTGC22417.09806157354618No Hit
CTTCCTCTTCTCAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCCTAAG3981.260610667680223No Hit
CTTCCTCTTCTCAGCAGTTGTTGTTTCCGTCCAACTGTAGAACCATGTCG2070.6556442417331814No Hit
GCTATCCTGCCATGCTCCGGACACTCCTGCAGCTCCAGCTTATCATTCAC1100.34840998352970987No Hit
AGACCCTGGAACTGCTACATGTGCGGGCACAAGGGTACCTACGGGCTGCT1070.3389078930698087No Hit
GCTATCTGCCATGCTCCAGACACTCCTGCAGCTCCAGCTTATCATTCACA990.31356898517673887No Hit
CTATCCTGCCATGCTCCAGACACTCCTGCAGCTCCAGCTTATCATTCACA860.27239325985050045No Hit
GTATCCTGCCATGCTCCAGACACTCCTGCAGCTCCAGCTTATCATTCACA560.17737235525148864No Hit
GCTATCCTGCATGCTCCAGACACTCCTGCAGCTCCAGCTTATCATTCACA540.17103762827822122No Hit
GCTATCCTGCCATGCTCCAGACACTCCCGCAGCTCCAGCTTATCATTCAC520.16470290130495374No Hit
GCTTCCTGCCATGCTCCAGACACTCCTGCAGCTCCAGCTTATCATTCACA500.15836817433168632No Hit
GCTATCCTGCCATGCTCCAGACACTCCTGCAGCCCCAGCTTATCATTCAC440.13936399341188394No Hit
AGACCCTGGAACTGCTACCTGTGCGGGCACAAGGGTACCTACGGGCTGCT410.12986190295198277No Hit
GCATCCTGCCATGCTCCAGACACTCCTGCAGCTCCAGCTTATCATTCACA400.12669453946534903No Hit
CTTCCTCTTCTCAGTGGGACAGGTGGGTAAACCTTTGGAGGGTCAAATTC380.12035981249208158No Hit
GCTATCCTGCCATGCTCCAGGCACTCCTGCAGCTCCAGCTTATCATTCAC370.11719244900544787No Hit
AGACCCCTGGAACTGCTACGTGTGCGGGCACAAGGGTACCTACGGGCTGC370.11719244900544787No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)