FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008143076

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008143076
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27648
Total Bases4 Mbp
Sequences flagged as poor quality0
Sequence length143-145
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTAGTGACAAGAGGGACATCTCGCGATTTCTCGAGTCCAACCCTGTGA1035637.45659722222222No Hit
ACAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGC803629.06539351851852No Hit
AGCACAGGACTCTTCTTTGGTATAAAGAGAGTACTTTTAAAGGTCCTGCT356612.897858796296296No Hit
CGGAAACAACAACTGCTGCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCT17266.242766203703703No Hit
CGGAAACAACAACTGCTGAGAAGAGGAAGCTGTTCAGACCAAGTCTCTGC2941.0633680555555556No Hit
CGTTAGTGACAAGGGGGACATCTCGCGATTTCTCGAGTCCAACCCTGTGA1310.47381365740740744No Hit
CGTTAGTGACAAGAGGGACATTTCGCGATTTCTCGAGTCCAACCCTGTGA890.3219039351851852No Hit
CAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT800.28935185185185186No Hit
CGTTAGTGACAAAGGGACATCTCGCGATTTCTCGAGTCCAACCCTGTGAT780.2821180555555555No Hit
CGTTAGTGACAGAGGGACATCTCGCGATTTCTCGAGTCCAACCCTGTGAT740.26765046296296297No Hit
ACACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT740.26765046296296297No Hit
CGTTAGTGAAAGAGGGACATCTCGCGATTTCTCGAGTCCAACCCTGTGAT550.19892939814814817No Hit
GTTAGTGACAAGAGGGACATCTCGCGATTTCTCGAGTCCAACCCTGTGAT540.1953125No Hit
AAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT520.1880787037037037No Hit
CGTAGTGACAAGAGGGACATCTCGCGATTTCTCGAGTCCAACCCTGTGAT500.1808449074074074No Hit
ACAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGTGC430.15552662037037038No Hit
ACAAAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT420.15190972222222224No Hit
CGTTGGTGACAAGAGGGACATCTCGCGATTTCTCGAGTCCAACCCTGTGA410.14829282407407407No Hit
GCACAGGACTCTTCTTTGGTATAAAGAGAGTACTTTTAAAGGTCCTGCTT390.14105902777777776No Hit
CGTTAGTGACAAGAGGGACATCTTGCGATTTCTCGAGTCCAACCCTGTGA370.13382523148148148No Hit
ACAACAGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT360.13020833333333331No Hit
CGTTGTGACAAGAGGGACATCTCGCGATTTCTCGAGTCCAACCCTGTGAT350.12659143518518517No Hit
CGGAAACAACACTGCTGCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTT340.12297453703703703No Hit
CGTTAGTGCAAGAGGGACATCTCGCGATTTCTCGAGTCCAACCCTGTGAT330.1193576388888889No Hit
ACAACAGGTCGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT320.11574074074074073No Hit
ACAACGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT320.11574074074074073No Hit
CGTTAGTGACAAGAGGGACTCTCGCGATTTCTCGAGTCCAACCCTGTGAT310.11212384259259259No Hit
CGTTAGTGACAAGAGGGAATCTCGCGATTTCTCGAGTCCAACCCTGTGAT310.11212384259259259No Hit
CTTAGTGACAAGAGGGACATCTCGCGATTTCTCGAGTCCAACCCTGTGAT300.10850694444444445No Hit
CGTTATGACAAGAGGGACATCTCGCGATTTCTCGAGTCCAACCCTGTGAT280.10127314814814814No Hit
CGTTAGTGACAAGAGGGACATCTCGCGATTTCTCGAGTCCAACCCTGTGG280.10127314814814814No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)