FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008143105

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008143105
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences111
Total Bases16 kbp
Sequences flagged as poor quality0
Sequence length143-145
%GC60

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCCTCTTCTCAGCAGTTGTTGTTTCCGTCCAACTGTAGAACCATGTCG8475.67567567567568No Hit
CTTCCTCTTCTCAGCTGTTGTTGTTTCCGTCCAACTGTAGAACCATGTCG32.7027027027027026No Hit
CTTCCTCTTCTCAGAGTTGTTGTTTCCGTCCAACTGTAGAACCATGTCGT21.8018018018018018No Hit
TGGCTCATCTTCAAACCGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21.8018018018018018No Hit
AGACCCCTGGAACTGCTACATTGCCTCTGCGTTGATACCACTGCCTCCCA10.9009009009009009Clontech SMART CDS Primer II A (95% over 22bp)
CTTCTCTTCTCAGCAGTTGTTGTTTCCGTCCAACTGTAGAACCATGTCGT10.9009009009009009No Hit
CTTCCTCTTCTCAGCAGTTGTGTTTCCGTCCAACTGTAGAACCATGTCGT10.9009009009009009No Hit
TGGCTCATCTTCAAACCGTCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10.9009009009009009No Hit
AGACCCCTGGAACTGCTACATTGCCTATCTACCTGGCGCGAGGGAGCCTC10.9009009009009009No Hit
CTTCCTCTTCTCAGCAGTTGTTGTTTCAGTCCAACTGTAGAACCATGTCG10.9009009009009009No Hit
CTCCTCTTCTCAGCTGGGAGCCTCAATGTTACCCTGCGTTGATACCACTG10.9009009009009009Clontech SMART CDS Primer II A (95% over 21bp)
CTTCCTCTTCGCAGCAGTTTTGTTTCCGTCCAACTGTAGAACCATGTCGT10.9009009009009009No Hit
CTTCCTCTTCTCAGCTGTCCCTCTTGTCACTAACGTCCAACTGTAGAACC10.9009009009009009No Hit
CTTCCTCTTCTCAGCTGGGAGCCTCAATGTTACCCTGCGTTGATACCACT10.9009009009009009No Hit
GCTATCCTGCCATGCTCCAGCAGTTGTTGTTTCCGTCCAACTGTAGAACC10.9009009009009009No Hit
CTTCCTCTTCTCAGATGTCCCTCTTGTCACTAATGTCCAACTGTAGAACC10.9009009009009009No Hit
CTTCCTCTTCTCAGATGTCCCTCTTGACTAACGTCCAACTGTAGAACCAT10.9009009009009009No Hit
TGGCTCATCTTCAAACCGTCTCCTGTTTTGTAGTCCAACCCTGTGATGAT10.9009009009009009No Hit
CTTCCTCTTCTCAGCAGTGTTGTTTCCGTCCAACTGTAGAACCATGTCGT10.9009009009009009No Hit
TGGCTCATCTTCAAACCGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10.9009009009009009No Hit
AGACCCCTGGAACTGCTACATTGCCTCTACCTGGCGCGAGGGAGCCTCAA10.9009009009009009No Hit
CTTCCTCTTCTCAACAGTTGTTGTTTCCGTCCAACTGTAGAACCATGTCG10.9009009009009009No Hit
CTTCCTCTTCTCAGCTGGGAGCCTCAATGTTACCCATGTACTCTGCGTTG10.9009009009009009No Hit
TGGCTCATCTTCAAACCGTCTGCATCTGACCTGTTGTTCCAACTGTAGAA10.9009009009009009No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)