FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008143131

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008143131
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15388
Total Bases2.2 Mbp
Sequences flagged as poor quality0
Sequence length143-145
%GC62

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTTCCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGCCG565136.72342084741357No Hit
CTTCCTCTTCTCAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCAAATT460329.91291915778529No Hit
CTTCCTCTTCTCAGCAGTTGTTGTTTCCGGGATTGTGTAGAACCATGTCG160710.443202495451No Hit
CTTCCTCTTCTCAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCCTAAG6764.393033532622823No Hit
CTTCCTCTTCGCAGCAGTTGTTGTTTCCGGGATTGTGTAGAACCATGTCG1811.1762412269300753No Hit
TCCTTCCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGCCGG1140.7408370158565116No Hit
CTTCCTCTTCTCAGCTGTTGTTGTTTCCGGGATTGTGTAGAACCATGTCG1020.6628541720821419No Hit
CTCCTTCCAGGTGCTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGCCGG910.591369898622303No Hit
CTCTTCCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGCCGG810.5263841954769951No Hit
CCCTTCCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGCCGG590.38341564855731736No Hit
CTCCTCCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGCCGG590.38341564855731736No Hit
CTTCCTCTTCTAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCAAATTC530.34442422667013256No Hit
CTCCTTCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGCCGG520.33792565635560173No Hit
CTTCCTCTTCTCAGTGGGACAGGTGGGTAAACCTTTGGAGGGTCAAATTC500.32492851572654013No Hit
CTTCCTCTCTCAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCAAATTC360.23394853132310892No Hit
CTTCCTCTTCTCGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCAAATTC340.2209513906940473No Hit
CTTCCTCTTTCAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCAAATTC340.2209513906940473No Hit
CTTCCTCTTCTCAGCAGTTGTTGTTCCGGGATTGTGTAGAACCATGTCGT320.2079542500649857No Hit
CTTCCTCTTCCCAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCAAATT320.2079542500649857No Hit
TTCCTCTTCTCAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCAAATTC290.1884585391213933No Hit
CTTCCTTTCTCAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCAAATTC270.1754613984923317No Hit
CTTCCTCTTCTCAGCTGGACAGGTGGGTAAACCTTTGGAGGGTCAAATTC260.16896282817780087No Hit
CTTCCTCTTCTCACTGGGACAGGTGGGTAAACCTTTGGAGGGTCAAATTC230.14946711723420847No Hit
CTTCTCTTCTCAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCAAATTC220.14296854691967767No Hit
CTTCCTCTTCTCAGCAGTTGTGTTTCCGGGATTGTGTAGAACCATGTCGT190.12347283597608527No Hit
CTTCCTCTTCTCAGCAGTTGTTGTTTCCGGATTGTGTAGAACCATGTCGT180.11697426566155446No Hit
CTCCTTCCAGGTGCATCTGACCTGTTGTGGATTGTGTAGAACCATGTCGT180.11697426566155446No Hit
CTTCCTCTTCTCAGCTGAGACAGGTGGGTAAACCTTTGGAGGGTCAAATT180.11697426566155446No Hit
CTTCCTCTTCTCAGCTGGGACGGGTGGGTAAACCTTTGGAGGGTCAAATT160.10397712503249285No Hit
CTCCTTCCAGGTGCTTTTGCGTGGAGTGTGTGGGCCTCTTGGTGGGGCCG160.10397712503249285No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)