FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008143153

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008143153
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11012
Total Bases1.5 Mbp
Sequences flagged as poor quality0
Sequence length140-145
%GC63

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTTCCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGCCG546549.62767889575009No Hit
CTTCCTCTTCTCAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCAAATT223820.323283690519432No Hit
CTTCCTCTTCTCAGCAGTTGTTGTTTCCGGGATTGTGTAGAACCATGTCG9388.517980385034509No Hit
CTTCCTCTTCGCAGCAGTTGTTGTTTCCGGGATTGTGTAGAACCATGTCG1601.452960406828914No Hit
CTTCCTCTTCTCAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCCTAAG1501.3621503814021068No Hit
TCCTTCCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGCCGG990.8990192517253905No Hit
CTCCTTCCAGGTGCTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGCCGG790.7173992008717762No Hit
CTTCCTCTTCTCAGCTGTTGTTGTTTCCGGGATTGTGTAGAACCATGTCG730.662913185615692No Hit
CTCTTCCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGCCGG680.6175081729022884No Hit
CTCCTTCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGCCGG600.5448601525608427No Hit
CCCTTCCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGCCGG460.41772611696331274No Hit
CTCCTCCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGCCGG400.3632401017072285No Hit
CTTCCTCTTCCCAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCAAATT260.23610606610969853No Hit
CTCCTTCCGGTGCTTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGCCGG200.18162005085361424No Hit
CTCCTTCCAGTGCTTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGCCGG180.16345804576825282No Hit
CTTCCTCTTCTAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCAAATTC180.16345804576825282No Hit
CTCCTTCCAGGTGCATCTGACCTGTTGTGGATTGTGTAGAACCATGTCGT180.16345804576825282No Hit
CTCCTTCCAGGTGCTTTTGCGTGGAGTGTGTGGGCCTCTTGGTGGGGCCG180.16345804576825282No Hit
CTTCCTCTTCTCGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCAAATTC170.1543770432255721No Hit
CTCCTTCCAGGTGCTTTTGCGTGGAGTGTGCGGACCTCTTGGTGGGGCCG160.1452960406828914No Hit
CTCCTTCCAGGTGCTTCTGACCTGTTGTGGATTGTGTAGAACCATGTCGT160.1452960406828914No Hit
CTCCTTCCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGCCC160.1452960406828914No Hit
CTCCTTCCAGGTGTTTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGCCG150.1362150381402107No Hit
CTTCCTCTTCTCAGTGGGACAGGTGGGTAAACCTTTGGAGGGTCAAATTC150.1362150381402107No Hit
TTCCTCTTCTCAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCAAATTC150.1362150381402107No Hit
CTCCTTCCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGACCG140.12713403559752998No Hit
CTCCTTCCAGGTGCTTTTGCGTGGAGTGCGTGGACCTCTTGGTGGGGCCG130.11805303305484927No Hit
CTTCCTTTCTCAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCAAATTC130.11805303305484927No Hit
CTCCTTCCAGGGCTTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGCCGG120.10897203051216856No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)