FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008143166

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008143166
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7022
Total Bases1 Mbp
Sequences flagged as poor quality0
Sequence length142-145
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGC407358.00341782967815No Hit
CGGAAACAACAACTGCTGAGAAGAGGAAGGGAAGTAGACCAAGTCTCTGC168924.052976360011392No Hit
CGGAAACAACAACTGCTGCGAAGAGGAAGGGAAGTAGACCAAGTCTCTGC1171.6661919681002564No Hit
CGGAAACAACAACAGCTGAGAAGAGGAAGGGAAGTAGACCAAGTCTCTGC831.1819994303617203No Hit
ACAACAGGTCAGATGCACCTGGAAGGAGGGAAGTAGACCAAGTCTCTGCT510.726288806607804No Hit
ACAACAGGTCAGAAGCACCTGGAAGGAGGGAAGTAGACCAAGTCTCTGCT320.4557106237539163No Hit
ACACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT310.4414696667616064No Hit
CGGAACAACAACTGCTGAGAAGAGGAAGGGAAGTAGACCAAGTCTCTGCT290.4129877527769866No Hit
AAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT260.370264881800057No Hit
ACAACAGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT260.370264881800057No Hit
GGAAACAACAACTGCTGAGAAGAGGAAGGGAAGTAGACCAAGTCTCTGCT250.3560239248077471No Hit
ACAAAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT220.3133010538308174No Hit
CGGAAACAACACTGCTGAGAAGAGGAAGGGAAGTAGACCAAGTCTCTGCT180.25633722586157787No Hit
CGGAAACACAACTGCTGAGAAGAGGAAGGGAAGTAGACCAAGTCTCTGCT160.22785531187695815No Hit
CGAAACAACAACTGCTGAGAAGAGGAAGGGAAGTAGACCAAGTCTCTGCT160.22785531187695815No Hit
ACAACAGGTCGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT150.21361435488464828No Hit
ACAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCCCCCACCCAGCGC150.21361435488464828No Hit
CAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT140.19937339789233835No Hit
CGGAAAAACAACTGCTGAGAAGAGGAAGGGAAGTAGACCAAGTCTCTGCT140.19937339789233835No Hit
ACAACAGGTCAGATCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT140.19937339789233835No Hit
ACAACGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT110.1566505269154087No Hit
ACAACAGGTCAGAGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT110.1566505269154087No Hit
ACAACAGGTAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT110.1566505269154087No Hit
CGGAAACAACAACTGCTGAGAAAGGAAGGGAAGTAGACCAAGTCTCTGCT110.1566505269154087No Hit
ACAACAGGTCAGTGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT110.1566505269154087No Hit
ACAACAGGTCAATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT100.14240956992309883No Hit
ACAACAGGTCAGATGAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT100.14240956992309883No Hit
CGGAAACAACAACTGCCGAGAAGAGGAAGGGAAGTAGACCAAGTCTCTGC100.14240956992309883No Hit
ACAACAGGTCAGATGCAGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT100.14240956992309883No Hit
ACAACAGGTCAGATGCAGGCCCATCGGCGTCCCAAGCTCCCACCCAGCGC90.12816861293078893No Hit
ACAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGTGC90.12816861293078893No Hit
CGGAAACAACAACTGCTGAGAAGAGGAGGGAAGTAGACCAAGTCTCTGCT80.11392765593847907No Hit
CGGAAACAACAATGCTGAGAAGAGGAAGGGAAGTAGACCAAGTCTCTGCT80.11392765593847907No Hit
ACAACAGGCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT80.11392765593847907No Hit
CGGAAACAACAACTCTGAGAAGAGGAAGGGAAGTAGACCAAGTCTCTGCT80.11392765593847907No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)