FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008143320

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008143320
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8227
Total Bases1.1 Mbp
Sequences flagged as poor quality0
Sequence length142-145
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGC515962.70815607147199No Hit
CGGAAACAACAACTGCTGAGAAGAGGAAGGGAAGTAGACCAAGTCTCTGC120814.683359669381305No Hit
CGGAAACAACAACTGCTGCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCT2743.33049714355172No Hit
CGGAAACAACAACTGCTGCGAAGAGGAAGGGAAGTAGACCAAGTCTCTGC1161.4099914914306553No Hit
CGGAAACAACAACTGCTGCAGGTGCTTTGCGTGGAGTGTGTGGACCTCTT1141.3856812933025404No Hit
CGGAAACAACAACAGCTGAGAAGAGGAAGGGAAGTAGACCAAGTCTCTGC861.045338519508934No Hit
ACAACAGGTCAGATGCACCTGGAAGGAGGGAAGTAGACCAAGTCTCTGCT700.850856934484016No Hit
ACACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT450.5469794578825817No Hit
AAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT370.44973866537012275No Hit
ACAACAGGTCAGAAGCACCTGGAAGGAGGGAAGTAGACCAAGTCTCTGCT370.44973866537012275No Hit
ACAACAGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT320.38896317004983594No Hit
CGGAAACAACAACTGCTGCGGCGGCGAGAGGACTGGCCCTCCCGGCTCCA310.37680807098577856No Hit
CGGAACAACAACTGCTGAGAAGAGGAAGGGAAGTAGACCAAGTCTCTGCT260.31603257566549164No Hit
ACAACAGGTCGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT240.2917223775373769No Hit
ACAAAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT240.2917223775373769No Hit
ACAACAGGTCAGATCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT240.2917223775373769No Hit
ACAACGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT200.24310198128114743No Hit
ACAACAGGTCAGATGCAGGCGCTGCCCATGGGAGCCCTGCCCCAGGGGCC200.24310198128114743No Hit
CGGAAACAACACTGCTGAGAAGAGGAAGGGAAGTAGACCAAGTCTCTGCT170.20663668408897531No Hit
ACAACAGGTCAGATGCAGGCCCATCGGCGTCCCAAGCTCCCACCCAGCGC170.20663668408897531No Hit
GGAAACAACAACTGCTGAGAAGAGGAAGGGAAGTAGACCAAGTCTCTGCT160.19448158502491797No Hit
ACAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCAC150.1823264859608606No Hit
ACAACAGGTAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT150.1823264859608606No Hit
CGAAACAACAACTGCTGAGAAGAGGAAGGGAAGTAGACCAAGTCTCTGCT140.17017138689680322No Hit
CAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT140.17017138689680322No Hit
ACAACAGGTCAGATGCAGGCCCATCGGTGCCCCAAGCTCCCACCCAGCGC120.14586118876868845No Hit
ACAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGTGC110.13370608970463108No Hit
CGGAAACAACAACTGCTGAGAAGAGGAAGGAAGTAGACCAAGTCTCTGCT100.12155099064057372No Hit
CGGAAACACAACTGCTGAGAAGAGGAAGGGAAGTAGACCAAGTCTCTGCT100.12155099064057372No Hit
ACAACAGGCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT100.12155099064057372No Hit
ACAACAGGTCAAAAGCACCTGGAAGGAGGGAAGTAGACCAAGTCTCTGCT100.12155099064057372No Hit
ACAACAGGTCAGAGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT100.12155099064057372No Hit
ACAACAGGTCAGATGCAGGCTCATCGGTGTCCCAAGCTCCCACCCAGCGC90.10939589157651634No Hit
CGGAAACAAAACTGCTGAGAAGAGGAAGGGAAGTAGACCAAGTCTCTGCT90.10939589157651634No Hit
ACAACAGGTCAATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT90.10939589157651634No Hit
ACAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCCCCCACCCAGCGC90.10939589157651634No Hit
ACAACAGGTCAGATGCAGGCCCATCGGTGTTCCAAGCTCCCACCCAGCGC90.10939589157651634No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)