FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008143327

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008143327
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18753
Total Bases2.7 Mbp
Sequences flagged as poor quality0
Sequence length142-145
%GC63

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTTCCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGCCG709737.84461152882205No Hit
CTTCCTCTTCTCAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCCTAAG519527.7022343091772No Hit
CTTCCTCTTCTCAGCAGTTGTTGTTTCCGGGATTGTGTAGAACCATGTCG231712.355356476297127No Hit
CTTCCTCTTCTCAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCAAATT4292.28763397856343No Hit
CTTCCTCTTCGCAGCAGTTGTTGTTTCCGGGATTGTGTAGAACCATGTCG1730.922519063616488No Hit
TCCTTCCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGCCGG1210.6452300965178905No Hit
CTCTTCCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGCCGG930.49592065269556873No Hit
CTCCTTCCAGGTGCTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGCCGG920.4905881725590572No Hit
CTTCCTCTTCTCAGCTGTTGTTGTTTCCGGGATTGTGTAGAACCATGTCG900.47992321228603424No Hit
CTCCTCCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGCCGG770.41060097051138483No Hit
CCCTTCCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGCCGG720.3839385698288274No Hit
CTCCTTCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGCCGG690.3679411294192929No Hit
CTTCCTCTTCTAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCCTAAGC520.27728896709859757No Hit
CTTCCTCTTCTCAGTGGGACAGGTGGGTAAACCTTTGGAGGGTCCTAAGC500.2666240068255746No Hit
CTTCCTCTTTCAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCCTAAGC450.23996160614301712No Hit
CTTCCTCTCTCAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCCTAAGC440.23462912600650562No Hit
CTTCTCTTCTCAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCCTAAGC430.22929664586999413No Hit
CTTCCTCTTCCCAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCCTAAG390.20796672532394817No Hit
CTTCCTTTCTCAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCCTAAGC360.1919692849144137No Hit
CTTCCTCTTCTCAGCAGTTGTTGTTCCGGGATTGTGTAGAACCATGTCGT350.1866368047779022No Hit
CTTCCTCTTCTCGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCCTAAGC350.1866368047779022No Hit
CTCCTTCCAGGTGCTTCTGACCTGTTGTGGATTGTGTAGAACCATGTCGT330.17597184450487924No Hit
CTCCTTCCAGGTGCATCTGACCTGTTGTGGATTGTGTAGAACCATGTCGT320.17063936436836774No Hit
TTCCTCTTCTCAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCCTAAGC280.14930944382232175No Hit
CTTCCTCTTCTCAGCAGTTGTGTTTCCGGGATTGTGTAGAACCATGTCGT280.14930944382232175No Hit
CTTCCTCTTCTCAGCAGTTGTTGTTTCGGGATTGTGTAGAACCATGTCGT280.14930944382232175No Hit
CTTCCTCTTCTCAGCAGTTGTTGTTTCCGGATTGTGTAGAACCATGTCGT270.14397696368581028No Hit
CTTCCTCTTCTCAGCGGGACAGGTGGGTAAACCTTTGGAGGGTCCTAAGC260.13864448354929879No Hit
CTCCTTCCAGGTGCTTTTGCGTGGAGTGTGTGGGCCTCTTGGTGGGGCCG250.1333120034127873No Hit
CTCCTTCCAGTGCTTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGCCGG240.1279795232762758No Hit
CTCCTCTTCTCAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCCTAAGC240.1279795232762758No Hit
CTTCCTCTTCTCAGCAGTTGTTTTTCCGGGATTGTGTAGAACCATGTCGT240.1279795232762758No Hit
CTCCTTCCAGGTGCTTTTCGTGGAGTGTGTGGACCTCTTGGTGGGGCCGG240.1279795232762758No Hit
CTTCCTCTTCTCAGCTGAGACAGGTGGGTAAACCTTTGGAGGGTCCTAAG240.1279795232762758No Hit
CTTCCTCTTCTCAGCAGTGTTGTTTCCGGGATTGTGTAGAACCATGTCGT230.1226470431397643No Hit
CTTCCTCTTCTCACTGGGACAGGTGGGTAAACCTTTGGAGGGTCCTAAGC230.1226470431397643No Hit
CTCCTTCCGGTGCTTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGCCGG210.11198208286674133No Hit
TTCCTCTTCTCAGCAGTTGTTGTTTCCGGGATTGTGTAGAACCATGTCGT210.11198208286674133No Hit
CTTCTCTTCTCAGCAGTTGTTGTTTCCGGGATTGTGTAGAACCATGTCGT200.10664960273022983No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)