FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008143342

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008143342
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9514
Total Bases1.3 Mbp
Sequences flagged as poor quality0
Sequence length143-145
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGC631666.38637796930838No Hit
CGGAAACAACAACTGCTGAGAAGAGGAAGGGAAGTAGACCAAGTCTCTGC127013.348749211688038No Hit
CGGAAACAACAACTGCTGCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCT2252.3649358839604795No Hit
CGGAAACAACAACTGCTGCGAAGAGGAAGGGAAGTAGACCAAGTCTCTGC1891.9865461425268027No Hit
CGGAAACAACAACAGCTGAGAAGAGGAAGGGAAGTAGACCAAGTCTCTGC1281.3453857473197395No Hit
ACACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT510.5360521336977087No Hit
ACAACAGGTCAGATGCACCTGGAAGGAGGGAAGTAGACCAAGTCTCTGCT420.4414546983392895No Hit
AAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT350.3678789152827412No Hit
ACAAAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT310.32583561067899935No Hit
ACAACAGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT290.30481395837712844No Hit
CAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT290.30481395837712844No Hit
ACAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCCCCCACCCAGCGC280.294303132226193No Hit
ACAACAGGTAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT270.2837923060752575No Hit
ACAACAGGTCAGAAGCACCTGGAAGGAGGGAAGTAGACCAAGTCTCTGCT250.2627706537733866No Hit
CGGAACAACAACTGCTGAGAAGAGGAAGGGAAGTAGACCAAGTCTCTGCT240.25225982762245114No Hit
ACAACAGGTCAATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT220.23123817532058022No Hit
ACAACAGGTCGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT210.22072734916964476No Hit
CGGAAACACAACTGCTGAGAAGAGGAAGGGAAGTAGACCAAGTCTCTGCT190.19970569686777379No Hit
ACAACAGGTCAGATGCAGGCCCACCGGTGTCCCAAGCTCCCACCCAGCGC190.19970569686777379No Hit
ACAACAGGTCAGATGCAGGCGCTGCCCATGGGAGCCCTGCCCCAGGGGCC190.19970569686777379No Hit
GGAAACAACAACTGCTGAGAAGAGGAAGGGAAGTAGACCAAGTCTCTGCT180.18919487071683833No Hit
ACAACGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT180.18919487071683833No Hit
ACAACAGGTCAGATGCAGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT180.18919487071683833No Hit
CGGAAACAACAACTGCTGCGGCGGCGAGAGGACTGGCCCTCCCGGCTCCA170.1786840445659029No Hit
ACAACAGGTCAGTGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT160.16817321841496743No Hit
ACAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGTGC140.1471515661130965No Hit
ACAACAGGCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT140.1471515661130965No Hit
ACAACAGGTCAGATGCGGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGC140.1471515661130965No Hit
CGGAAACAACAACTGCTGAGAAGAGGAAGGAAGTAGACCAAGTCTCTGCT130.13664073996216103No Hit
ACAACAGGTCAGATCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT130.13664073996216103No Hit
ACAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCAC120.12612991381122557No Hit
ACAACAGGTCAGATGAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT110.11561908766029011No Hit
ACAACAGGTCAGATGCAGGCCCGTCGGTGTCCCAAGCTCCCACCCAGCGC110.11561908766029011No Hit
ACAACAGGTCAGATGCAGGCCCATCGGTGTCCCGAGCTCCCACCCAGCGC110.11561908766029011No Hit
ACAACAGGTCAGATGCAGGCCCATCGGCGTCCCAAGCTCCCACCCAGCGC110.11561908766029011No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)