FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008143372

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008143372
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18303
Total Bases2.6 Mbp
Sequences flagged as poor quality0
Sequence length142-145
%GC64

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGC698038.135824728186634No Hit
CGGAAACAACAACTGCTGCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCT677537.01578976124132No Hit
CGGAAACAACAACTGCTGAGAAGAGGAAGGGAAGTAGACCAAGTCTCTGC13737.501502485931268No Hit
CGGAAACAACAACTGCTGCGAAGAGGAAGGGAAGTAGACCAAGTCTCTGC2621.4314593236081516No Hit
CGGAAACAACAACAGCTGAGAAGAGGAAGGGAAGTAGACCAAGTCTCTGC1530.8359285363055237No Hit
CGGAACAACAACTGCTGCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTT1120.6119215429164617No Hit
GGAAACAACAACTGCTGCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTT920.5026498388242364No Hit
CGGAAACACAACTGCTGCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTT820.44801398677812376No Hit
CGAAACAACAACTGCTGCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTT650.35513303829973225No Hit
ACACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT640.34966945309512104No Hit
CGGAAACAACAACTGCTGCGGCGGCGAGAGGACTGGCCCTCCCGGCTCCA550.3004971862536196No Hit
AAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT500.2731792602305633No Hit
CGGAAACAACACTGCTGCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTT500.2731792602305633No Hit
CGGAAACAAAACTGCTGCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTT420.22947057859367317No Hit
ACAAAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT420.22947057859367317No Hit
ACAACAGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT390.21307982297983935No Hit
ACAACAGGTCGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT370.20215265257061682No Hit
CGGAAAAACAACTGCTGCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTT360.19668906736600558No Hit
CGGAAACAACAACTGCTGCAGGTGCCTTTGCGTGGAGTGTGTGGACCTCT310.16937114134294923No Hit
ACAACAGGTCAGATGCAGGCCCATCGGTGCCCCAAGCTCCCACCCAGCGC280.15298038572911543No Hit
CGGAAACAACAACTCTGCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTT270.1475168005245042No Hit
CGGAAACAACAACTGCTGCGGGTGCTTTTGCGTGGAGTGTGTGGACCTCT270.1475168005245042No Hit
ACAACGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT260.14205321531989293No Hit
CGGAAACACAACTGCTGAGAAGAGGAAGGGAAGTAGACCAAGTCTCTGCT240.13112604491067037No Hit
CGGAAACAACAACTGCTGCAGGTGCTTTTGCGTGGAGTGTGTGGGCCTCT230.1256624597060591No Hit
ACAACAGGTAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT230.1256624597060591No Hit
CAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT220.12019887450144785No Hit
CGGAAACAACAACTGCTGCAGGTGCTTTTGCGTGGGGTGTGTGGACCTCT210.11473528929683659No Hit
CGGAAACAACAATGCTGCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTT200.10927170409222532No Hit
ACAACAGGTCAGATGCAGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT200.10927170409222532No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)