FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008143402

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008143402
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10396
Total Bases1.5 Mbp
Sequences flagged as poor quality0
Sequence length143-145
%GC66

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGC764773.55713736052328No Hit
CGGAAACAACAACTGCTGAGAAGAGGAAGGGAAGTAGACCAAGTCTCTGC107710.359753751442863No Hit
CGGAAACAACAACTGCTGCGAAGAGGAAGGGAAGTAGACCAAGTCTCTGC1141.0965756060023086No Hit
CGGAAACAACAACAGCTGAGAAGAGGAAGGGAAGTAGACCAAGTCTCTGC970.9330511735282802No Hit
ACACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT690.6637168141592921No Hit
CGGAAACAACAACTGCTGCGGCGGCGAGAGGACTGGCCCTCCCGGCTCCA670.6444786456329357No Hit
AAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT540.5194305502116198No Hit
ACAACAGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT500.4809542131589073No Hit
ACAAAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT360.34628703347441325No Hit
ACAACGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT310.2981916121585225No Hit
ACAACAGGCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT290.27895344363216623No Hit
ACAACAGGTAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT270.2597152751058099No Hit
CAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT260.2500961908426318No Hit
ACAACAGGTCGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT250.24047710657945365No Hit
ACAACAGGTCAGATGCACCTGGAAGGAGGGAAGTAGACCAAGTCTCTGCT250.24047710657945365No Hit
ACAACAGGTCAGATGCAGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT250.24047710657945365No Hit
ACAACAGGTCAGTGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT250.24047710657945365No Hit
ACAACAGGTCAGATGCAGGCCCATCGGTGCCCCAAGCTCCCACCCAGCGC230.22123893805309736No Hit
ACAACAGGTCAGATCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT210.20200076952674104No Hit
ACAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGTGC200.19238168526356292No Hit
ACAACAGGTCAATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT200.19238168526356292No Hit
ACAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCCCCCACCCAGCGC200.19238168526356292No Hit
CGGAACAACAACTGCTGAGAAGAGGAAGGGAAGTAGACCAAGTCTCTGCT180.17314351673720663No Hit
GGAAACAACAACTGCTGAGAAGAGGAAGGGAAGTAGACCAAGTCTCTGCT170.16352443247402848No Hit
ACAACAGGTCAGATGCAGGCCCATCGGCGTCCCAAGCTCCCACCCAGCGC170.16352443247402848No Hit
ACAACAGGTCAGAAGCACCTGGAAGGAGGGAAGTAGACCAAGTCTCTGCT170.16352443247402848No Hit
ACAACAGGTCAGATGCAGGCCCATTGGTGTCCCAAGCTCCCACCCAGCGC140.13466717968449404No Hit
ACAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAACTCCCACCCAGCGC140.13466717968449404No Hit
ACAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCAC130.1250480954213159No Hit
ACAACAGGTCAGATGCAGGCCCATCGGTGTCCCGAGCTCCCACCCAGCGC130.1250480954213159No Hit
ACAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCGCCCAGCGC130.1250480954213159No Hit
ACAACAGGTCAGATGAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT120.11542901115813775No Hit
ACAACAGGTCAGATGCGGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGC120.11542901115813775No Hit
ACAACAGGTCAGATGCAGGCCCACCGGTGTCCCAAGCTCCCACCCAGCGC120.11542901115813775No Hit
ACAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGTTCCCACCCAGCGC120.11542901115813775No Hit
CGAAACAACAACTGCTGAGAAGAGGAAGGGAAGTAGACCAAGTCTCTGCT110.10580992689495959No Hit
ACAACAGGTCAGATGCGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT110.10580992689495959No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)