FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008143472

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008143472
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9817
Total Bases1.4 Mbp
Sequences flagged as poor quality0
Sequence length142-145
%GC66

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGC716172.94489151471937No Hit
CGGAAACAACAACTGCTGAGAAGAGGAAGGGAAGTAGACCAAGTCTCTGC108911.093001935418153No Hit
CGGAAACAACAACTGCTGCGAAGAGGAAGGGAAGTAGACCAAGTCTCTGC960.9778954874197819No Hit
CGGAAACAACAACTGCTGCGGCGGCGAGAGGACTGGCCCTCCCGGCTCCA850.8658449628195987No Hit
CGGAAACAACAACAGCTGAGAAGAGGAAGGGAAGTAGACCAAGTCTCTGC670.6824895589283895No Hit
ACACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT640.6519303249465213No Hit
AAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT450.4583885097280228No Hit
ACAAAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT420.4278292757461546No Hit
ACAACAGGTCAGATGCACCTGGAAGGAGGGAAGTAGACCAAGTCTCTGCT420.4278292757461546No Hit
ACAACGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT300.3055923398186819No Hit
CAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT300.3055923398186819No Hit
ACAACAGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT290.29540592849139247No Hit
ACAACAGGCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT290.29540592849139247No Hit
ACAACAGGTCGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT230.23428746052765612No Hit
ACAACAGGTAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT220.22410104920036672No Hit
ACAACAGGTCAGAAGCACCTGGAAGGAGGGAAGTAGACCAAGTCTCTGCT220.22410104920036672No Hit
ACAACAGGTCAGATGCAGGCCCATCGGCGTCCCAAGCTCCCACCCAGCGC180.18335540389120913No Hit
ACAACAGGTCAGATCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT180.18335540389120913No Hit
ACAACAGGTCAGAGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT160.16298258123663031No Hit
CGGAACAACAACTGCTGAGAAGAGGAAGGGAAGTAGACCAAGTCTCTGCT160.16298258123663031No Hit
ACAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCCCCCACCCAGCGC160.16298258123663031No Hit
ACAACAGGTCAATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT150.15279616990934095No Hit
ACAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGTGC150.15279616990934095No Hit
ACAACAGGTCAGATGCAGGCCCATCGGTGCCCCAAGCTCCCACCCAGCGC150.15279616990934095No Hit
CGGAAACAACACTGCTGAGAAGAGGAAGGGAAGTAGACCAAGTCTCTGCT140.14260975858205155No Hit
ACAACAGGTCAGATGCAGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT140.14260975858205155No Hit
ACAACAGGTCAGATGCAGGCCCACCGGTGTCCCAAGCTCCCACCCAGCGC120.12223693592747274No Hit
CGAAACAACAACTGCTGAGAAGAGGAAGGGAAGTAGACCAAGTCTCTGCT120.12223693592747274No Hit
ACAACAGGTCAGATGAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT110.11205052460018336No Hit
CGGAAACACAACTGCTGAGAAGAGGAAGGGAAGTAGACCAAGTCTCTGCT110.11205052460018336No Hit
ACAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAACGC110.11205052460018336No Hit
ACAACAGGTCAGATGCGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT110.11205052460018336No Hit
ACAACAGGTCAGATGTAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGC110.11205052460018336No Hit
ACAACAGGTCAGATGCAGGTCCATCGGTGTCCCAAGCTCCCACCCAGCGC100.10186411327289396No Hit
ACAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCAC100.10186411327289396No Hit
GGAAACAACAACTGCTGAGAAGAGGAAGGGAAGTAGACCAAGTCTCTGCT100.10186411327289396No Hit
ACAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCACCCACCCAGCGC100.10186411327289396No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)