FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008143492

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008143492
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12240
Total Bases1.7 Mbp
Sequences flagged as poor quality0
Sequence length142-145
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGC713258.26797385620915No Hit
CGGAAACAACAACTGCTGAGAAGAGGAAGGGAAGTAGACCAAGTCTCTGC182214.88562091503268No Hit
CGGAAACAACAACTGCTGCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCT9958.129084967320262No Hit
CGGAAACAACAACTGCTGCGAAGAGGAAGGGAAGTAGACCAAGTCTCTGC2341.911764705882353No Hit
CGGAAACAACAACAGCTGAGAAGAGGAAGGGAAGTAGACCAAGTCTCTGC1431.1683006535947713No Hit
CGGAAACAACAACTGCTGCGGCGGCGAGAGGACTGGCCCTCCCGGCTCCA990.8088235294117648No Hit
ACACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT570.46568627450980393No Hit
ACAACAGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT480.39215686274509803No Hit
AAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT450.3676470588235294No Hit
ACAACAGGTCAGATGCACCTGGAAGGAGGGAAGTAGACCAAGTCTCTGCT420.3431372549019608No Hit
ACAACAGGTCGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT380.3104575163398693No Hit
ACAAAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT360.29411764705882354No Hit
ACAACAGGTAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT340.2777777777777778No Hit
CAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT300.24509803921568626No Hit
CGGAACAACAACTGCTGAGAAGAGGAAGGGAAGTAGACCAAGTCTCTGCT290.2369281045751634No Hit
ACAACGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT270.22058823529411764No Hit
ACAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCCCCCACCCAGCGC270.22058823529411764No Hit
ACAACAGGTCAATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT250.20424836601307192No Hit
ACAACAGGTCAGATGCAGGCCCATCGGCGTCCCAAGCTCCCACCCAGCGC220.17973856209150327No Hit
ACAACAGGTCAGAAGCACCTGGAAGGAGGGAAGTAGACCAAGTCTCTGCT210.1715686274509804No Hit
ACAACAGGTCAGATGCAGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT210.1715686274509804No Hit
GGAAACAACAACTGCTGAGAAGAGGAAGGGAAGTAGACCAAGTCTCTGCT200.16339869281045752No Hit
ACAACAGGTCAGATGCAGGCCCATCGGTGCCCCAAGCTCCCACCCAGCGC190.15522875816993464No Hit
ACAACAGGTCAGATCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT190.15522875816993464No Hit
CGGAAACACAACTGCTGAGAAGAGGAAGGGAAGTAGACCAAGTCTCTGCT180.14705882352941177No Hit
ACAACAGGTCAGTGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT180.14705882352941177No Hit
CGGAAAAACAACTGCTGAGAAGAGGAAGGGAAGTAGACCAAGTCTCTGCT170.1388888888888889No Hit
CGGAAACAACACTGCTGAGAAGAGGAAGGGAAGTAGACCAAGTCTCTGCT170.1388888888888889No Hit
CGGAACAACAACTGCTGCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTT170.1388888888888889No Hit
CGGAAACAACAACTGCTGAGAAGAGGAAGGAAGTAGACCAAGTCTCTGCT160.130718954248366No Hit
ACAACAGGCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT160.130718954248366No Hit
CGAAACAACAACTGCTGAGAAGAGGAAGGGAAGTAGACCAAGTCTCTGCT160.130718954248366No Hit
ACAACAGGTCAGATGCGGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGC150.12254901960784313No Hit
ACAACAGGTCAGATGCGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT140.11437908496732027No Hit
ACAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCAC130.10620915032679738No Hit
ACAACAGGTCAGATGCAGGCCCATCGGTGTCCCAGGCTCCCACCCAGCGC130.10620915032679738No Hit
CGGAAACAAAACTGCTGAGAAGAGGAAGGGAAGTAGACCAAGTCTCTGCT130.10620915032679738No Hit
CGGAAACAACAACTGCTGAGAAGAGAAGGGAAGTAGACCAAGTCTCTGCT130.10620915032679738No Hit
GGAAACAACAACTGCTGCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTT130.10620915032679738No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)