FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008143539

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008143539
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10180
Total Bases1.4 Mbp
Sequences flagged as poor quality0
Sequence length142-145
%GC64

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTTCCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGCCG685667.34774066797642No Hit
CTTCCTCTTCTCAGCAGTTGTTGTTTCCGGGATTGTGTAGAACCATGTCG9138.968565815324165No Hit
CTTCCTCTTCTCAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCCTAAG1701.6699410609037328No Hit
CTTCCTCTTCGCAGCAGTTGTTGTTTCCGGGATTGTGTAGAACCATGTCG1491.4636542239685657No Hit
TCCTTCCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGCCGG1311.2868369351669942No Hit
CTTCCTCTTCTCAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCAAATT1291.2671905697445973No Hit
CTCTTCCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGCCGG1021.0019646365422397No Hit
CTTCCTCTTCTCAGCTGTTGTTGTTTCCGGGATTGTGTAGAACCATGTCG1000.9823182711198428No Hit
CTCCTTCCAGGTGCTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGCCGG860.8447937131630648No Hit
CTCCTTCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGCCGG830.8153241650294695No Hit
CTCCTCCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGCCGG800.7858546168958742No Hit
CCCTTCCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGCCGG600.5893909626719057No Hit
CTCCTTCCAGGTGCATCTGACCTGTTGTGGATTGTGTAGAACCATGTCGT470.4616895874263261No Hit
CTCCTTCCAGGTGCTTCTGACCTGTTGTGGATTGTGTAGAACCATGTCGT280.275049115913556No Hit
CTCCTTCCAGTGCTTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGCCGG260.2554027504911591No Hit
CTCCTTCCAGGTGCTTTTGCGTGGAGTGTGCGGACCTCTTGGTGGGGCCG240.2357563850687623No Hit
CTCCTTCCGGTGCTTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGCCGG210.20628683693516697No Hit
CTCCTTCCAGGTGTTTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGCCG190.18664047151277013No Hit
CTCCTTCCAGGTGCTTTTCGTGGAGTGTGTGGACCTCTTGGTGGGGCCGG190.18664047151277013No Hit
CTCCTTCCAGGTGCTTTTGCGTGGGGTGTGTGGACCTCTTGGTGGGGCCG160.15717092337917485No Hit
CTCCTTCCAGGTGTTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGCCGG140.137524557956778No Hit
CTCCTTCCAGGTGCTTTTGCGTGGAGTGTGTGGACCCCTTGGTGGGGCCG140.137524557956778No Hit
CTTCCTCTTCTCAGCAGTTGTTGTTCCGGGATTGTGTAGAACCATGTCGT130.12770137524557956No Hit
CTCCTTCCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGCCC130.12770137524557956No Hit
CTCCTTCCAGGTGCTTTTGCGTGGAGTGTGTGGGCCTCTTGGTGGGGCCG130.12770137524557956No Hit
CTCCTTCCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGCTG120.11787819253438114No Hit
CTTCCTCTTCTCAGCAGTTGTGTTTCCGGGATTGTGTAGAACCATGTCGT120.11787819253438114No Hit
CTCCTTCCAGGTGCTTTTGTGTGGAGTGTGTGGACCTCTTGGTGGGGCCG110.10805500982318272No Hit
CTCCTTCCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGTCG110.10805500982318272No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)