FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008143583

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008143583
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1951
Total Bases282.8 kbp
Sequences flagged as poor quality0
Sequence length143-145
%GC62

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCCTCTTCTCAGCAGTTGTTGTTTCCGGGATTGTGTAGAACCATGTCG122762.89082521783701No Hit
CTTCCTCTTCGCAGCAGTTGTTGTTTCCGGGATTGTGTAGAACCATGTCG1246.355715017939518No Hit
CTTCCTCTTCTCAGCTGTTGTTGTTTCCGGGATTGTGTAGAACCATGTCG914.664274730907227No Hit
CTCCTTCCAGGTGCATCTGACCTGTTGTGGATTGTGTAGAACCATGTCGT412.1014864172219374No Hit
CTCCTTCCAGGTGCTTCTGACCTGTTGTGGATTGTGTAGAACCATGTCGT371.8964633521271141No Hit
CTCCTTCCAGGTGCTTTTGCTCCAAGGAGTGCCTGCTCATTTCAAACAGG211.0763710917478215No Hit
CTTCCTCTTCTCAGCAGTTGTTGTTTCCGGATTGTGTAGAACCATGTCGT211.0763710917478215No Hit
CTTCCTCTTCTCAGCAGTTGTTGTTCCGGGATTGTGTAGAACCATGTCGT201.0251153254741157No Hit
CTTCCTCTTCTCAGCAGTTGTTGTTTCGGGATTGTGTAGAACCATGTCGT170.8713480266529985No Hit
CTTCCTCTTCTCAGCAGTTGTGTTTCCGGGATTGTGTAGAACCATGTCGT130.6663249615581752No Hit
CTTCCTCTTCTCAGCAGTTGTTTTTCCGGGATTGTGTAGAACCATGTCGT120.6150691952844696No Hit
CTCCTTCCAGGTGCTTTTGACCTGTTGTGGATTGTGTAGAACCATGTCGT90.4613018964633521No Hit
CTTCTCTTCTCAGCAGTTGTTGTTTCCGGGATTGTGTAGAACCATGTCGT80.4100461301896463No Hit
CTTCCTCTTCTCAGTAGTTGTTGTTTCCGGGATTGTGTAGAACCATGTCG80.4100461301896463No Hit
CTTCCTCTTCCCAGCAGTTGTTGTTTCCGGGATTGTGTAGAACCATGTCG70.35879036391594055No Hit
CTTCCTCTTCTCAGCAGCAGTTGTTGTTTCCGGGATTGTGTAGAACCATG70.35879036391594055No Hit
CTTCCTCTTCTCAGCGGTTGTTGTTTCCGGGATTGTGTAGAACCATGTCG60.3075345976422348No Hit
CTTCCTCTTCTCAGAGTTGTTGTTTCCGGGATTGTGTAGAACCATGTCGT60.3075345976422348No Hit
TTCCTCTTCTCAGCAGTTGTTGTTTCCGGGATTGTGTAGAACCATGTCGT60.3075345976422348No Hit
CTCCTTCCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGCCG50.2562788313685289No Hit
CTTCCTCTTCTCAGCAGTGTTGTTTCCGGGATTGTGTAGAACCATGTCGT50.2562788313685289No Hit
CTTCCTCTTCTCAGCATTGTTGTTTCCGGGATTGTGTAGAACCATGTCGT50.2562788313685289No Hit
CTCCTCTTCTCAGCAGTTGTTGTTTCCGGGATTGTGTAGAACCATGTCGT50.2562788313685289No Hit
CTCCTTCCAGGTGCTTTGCGTTGATACCACTGCTTCCCATGTACTCTGCG50.2562788313685289Clontech Universal Primer Mix Long (95% over 22bp)
CTTCCTCTTCTCAGCGTTGTTGTTTCCGGGATTGTGTAGAACCATGTCGT40.20502306509482315No Hit
CTTCCTCTTCTCAGCAGTTGTTGTTTCCGGGATTGTGTAGAACCATGTCT40.20502306509482315No Hit
CTTCCTTTCTCAGCAGTTGTTGTTTCCGGGATTGTGTAGAACCATGTCGT40.20502306509482315No Hit
CTCCTTCCAGGTGCTTTTGCGTTGATACCACTGCTTCCCATGTACTCTGC40.20502306509482315Clontech Universal Primer Mix Long (95% over 21bp)
CTTCCTCTTCTCAGCAGTTGTTGTTTCCGGGATTGTGTAAGAACCATGTC40.20502306509482315No Hit
CTTCCTCTTCTCAGCAGTTGTTGTTTCAGGGATTGTGTAGAACCATGTCG40.20502306509482315No Hit
CTTCCTCTTCGCAGCAGTTGTTGTTCCGGGATTGTGTAGAACCATGTCGT30.1537672988211174No Hit
CTTCCTCTTCTCAGCAGTTGTTGTTTCCGGGATTGTGTAGAACCATCTCG30.1537672988211174No Hit
CTTCCTCTTCTCAGCAGTTTTGTTTCCGGGATTGTGTAGAACCATGTCGT30.1537672988211174No Hit
CTTCCTCTTCTCAACAGTTGTTGTTTCCGGGATTGTGTAGAACCATGTCG30.1537672988211174No Hit
CTTCCTCTTCGCAGCAGTGTTGTTTCCGGGATTGTGTAGAACCATGTCGT30.1537672988211174No Hit
CTTCCTCTCTCAGCAGTTGTTGTTTCCGGGATTGTGTAGAACCATGTCGT30.1537672988211174No Hit
CTTCCTCTTCTCAGCAGTTGTTGTTTCCAGGATTGTGTAGAACCATGTCG20.10251153254741158No Hit
CTCCTTCCAGGTGCTTTTCGTTGATACCACTGCTCCCCATGTACTCTGCG20.10251153254741158No Hit
CTTCCTCTTCTCAGCAGTTGTTGTTTCCGGGATTGTGTAGAACCATGTCC20.10251153254741158No Hit
CTTCCTCTTCTCAGAAGTTGTTGTTTCCGGGATTGTGTATAACCATGTCG20.10251153254741158No Hit
CTTCCTCTTCTCGGCAGTTGTTGTTTCCGGGATTGTGTAGAACCATGTCG20.10251153254741158No Hit
CTTCCTCTTCTCAGCAGTTGTTGTTTCCGGGATTGTGTAGAACCATGTAG20.10251153254741158No Hit
CTTCCTCTTCGCAGCAGTTGTTGTTTCGGGATTGTGTAGAACCATGTCGT20.10251153254741158No Hit
CTCCTTCCAGGTGCTTTTGCAATGTGGCAAGAAAATTTCAACATATGCCG20.10251153254741158No Hit
CTTCCTCCTCTCAGCAGTTGTTGTTTCCGGGATTGTGTAGAACCATGTCG20.10251153254741158No Hit
CTCCTTCCAGGTGCTTCTGACTGTTGTGGATTGTGTAGAACCATGTCGTC20.10251153254741158No Hit
CTCCTTCCAGGTGCTCTGACCTGTTGTGGATTGTGTAGAACCATGTCGTC20.10251153254741158No Hit
CTTCCTCTTCTCAGCAGTTGTTGTTTCCGGGATTGTGTAGAACCATGACG20.10251153254741158No Hit
CTTCCTCTTCTCAGCAGTTGTTGTCCGGGATTGTGTAGAACCATGTCGTC20.10251153254741158No Hit
CTTCCTCTTCTCAGCAGTTGTTGTTTACGGGATTGTGTAGAACCATGTCG20.10251153254741158No Hit
CTTCCTCTTCTCAGCAGTTGTTGTTTCCGGGATTGTGTATAACCATGTCG20.10251153254741158No Hit
CTTCCTCTTCTCAGCAGTTGATGTTTCCGGGATTGTGTAGAACCATGTCG20.10251153254741158No Hit
CTCCTTCCAGGTGCTTTGACCTGTTGTGGATTGTGTAGAACCATGTCGTC20.10251153254741158No Hit
CTCCTTCCAGGTGCTTTTGCATCTGACCTGTTGTGGATTGTGTAGAACCA20.10251153254741158No Hit
CTTCCTCTTCGCAGCAGTTGTTGTTTCAGGGATTGTGTAGAACCATGTCG20.10251153254741158No Hit
CTTCCTCTTCTCAGAAGTTGTTGTTTCCGGGATTGTGTAGAACCATGTCG20.10251153254741158No Hit
CTTCCTCTTCTCAGCTGTTGTTGTTCCGGGATTGTGTAGAACCATGTCGT20.10251153254741158No Hit
TTCCTCTTCTCAGCAGTTGTTGTTTCCGGATTGTGTAGAACCATGTCGTC20.10251153254741158No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)