FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008143745

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008143745
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18183
Total Bases2.6 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC62

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTTCCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGCCG721439.67442116262443No Hit
CTTCCTCTTCTCAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCAAATT489926.942748721333114No Hit
CTTCCTCTTCTCAGCAGTTGTTGTTTCCGGGATTGTGTAGAACCATGTCG187210.295330803497773No Hit
CTTCCTCTTCTCAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCCTAAG6053.327283726557774No Hit
CTTCCTCTTCGCAGCAGTTGTTGTTTCCGGGATTGTGTAGAACCATGTCG2641.4519056261343013No Hit
CTTCCTCTTCTCAGCTGTTGTTGTTTCCGGGATTGTGTAGAACCATGTCG1300.7149535280206787No Hit
TCCTTCCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGCCGG1100.6049606775559588No Hit
CTCTTCCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGCCGG960.527965682230655No Hit
CTCCTTCCAGGTGCTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGCCGG830.4564703294285872No Hit
CTCCTTCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGCCGG810.44547104438211516No Hit
CTCCTCCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGCCGG810.44547104438211516No Hit
CTTCCTCTTCTAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCAAATTC570.3134796238244514No Hit
CTTCCTCTTCTCAGTGGGACAGGTGGGTAAACCTTTGGAGGGTCAAATTC550.3024803387779794No Hit
CCCTTCCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGCCGG520.2859814112082715No Hit
CTTCCTCTTCCCAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCAAATT480.2639828411153275No Hit
CTCCTTCCAGGTGCATCTGACCTGTTGTGGATTGTGTAGAACCATGTCGT440.24198427102238357No Hit
TTCCTCTTCTCAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCAAATTC410.2254853434526756No Hit
CTTCCTCTTCTCGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCAAATTC390.21448605840620358No Hit
CTTCCTCTTCTCAGCAGTTGTTGTTCCGGGATTGTGTAGAACCATGTCGT360.1979871308364956No Hit
CTTCCTCTTTCAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCAAATTC330.18148820326678766No Hit
CTTCCTCTCTCAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCAAATTC300.1649892756970797No Hit
CTTCCTCTTCTCACTGGGACAGGTGGGTAAACCTTTGGAGGGTCAAATTC290.1594896331738437No Hit
CTTCTCTTCTCAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCAAATTC290.1594896331738437No Hit
CTTCCTCTTCTCAGCTGGACAGGTGGGTAAACCTTTGGAGGGTCAAATTC290.1594896331738437No Hit
CTCCTTCCAGGTGCTTCTGACCTGTTGTGGATTGTGTAGAACCATGTCGT270.14849034812737172No Hit
CTCCTTCCGGTGCTTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGCCGG270.14849034812737172No Hit
CTTCCTCTTCTCAGCAGTTGTTGTTTCCGGATTGTGTAGAACCATGTCGT250.13749106308089976No Hit
CTTCCTCTTCTCAGCAGTTGTGTTTCCGGGATTGTGTAGAACCATGTCGT250.13749106308089976No Hit
CTCCTTCCAGGTGCTTTTCGTGGAGTGTGTGGACCTCTTGGTGGGGCCGG240.13199142055766375No Hit
CTCCTTCCAGTGCTTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGCCGG230.12649177803442777No Hit
CTTCCTTTCTCAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCAAATTC230.12649177803442777No Hit
CTTCCTCTTCTCAGCGGGACAGGTGGGTAAACCTTTGGAGGGTCAAATTC200.1099928504647198No Hit
CTCCTTCCAGGTGCTTTTGCGTGGAGTGTGTGGGCCTCTTGGTGGGGCCG190.10449320794148381No Hit
CTTCCTCTTCTCAGCTGAGACAGGTGGGTAAACCTTTGGAGGGTCAAATT190.10449320794148381No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)