FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008143807

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008143807
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31774
Total Bases4.6 Mbp
Sequences flagged as poor quality0
Sequence length142-145
%GC61

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCCTCTTCTCAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCAAATT1560049.096745766979296No Hit
CTCCTTCCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGCCG686121.593126455592625No Hit
CTTCCTCTTCTCAGCAGTTGTTGTTTCCGGGATTGTGTAGAACCATGTCG22307.018316862843835No Hit
CTTCCTCTTCTCAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCCTAAG8902.8010322905520235No Hit
CTTCCTCTTCGCAGCAGTTGTTGTTTCCGGGATTGTGTAGAACCATGTCG2720.8560458236293824No Hit
CTTCCTCTTCTCAGTGGGACAGGTGGGTAAACCTTTGGAGGGTCAAATTC1810.56964813998867No Hit
CTTCCTCTTCTAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCAAATTC1750.5507647762321395No Hit
CTTCCTCTTTCAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCAAATTC1170.36822559325234466No Hit
TCCTTCCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGCCGG1140.35878391137407945No Hit
CTTCCTCTTCCCAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCAAATT1120.35248945678856924No Hit
CTTCCTCTTCTCGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCAAATTC1110.3493422294958142No Hit
CTTCCTCTTCTCAGCTGTTGTTGTTTCCGGGATTGTGTAGAACCATGTCG1100.3461950022030591No Hit
CTCCTTCCAGGTGCTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGCCGG1040.3273116384465286No Hit
TTCCTCTTCTCAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCAAATTC880.2769560017624473No Hit
CTTCTCTTCTCAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCAAATTC850.267514319884182No Hit
CTTCCTCTTCTCAGCTGGACAGGTGGGTAAACCTTTGGAGGGTCAAATTC790.24863095612765151No Hit
CTTCCTCTCTCAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCAAATTC790.24863095612765151No Hit
CTTCCTCTTCTCACTGGGACAGGTGGGTAAACCTTTGGAGGGTCAAATTC770.24233650154214134No Hit
CTTCCTTTCTCAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCAAATTC760.2391892742493863No Hit
CTCCTCCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGCCGG760.2391892742493863No Hit
CTCTTCCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGCCGG750.23604204695663122No Hit
CTCCTTCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGCCGG690.21715868320010073No Hit
CCCTTCCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGCCGG640.2014225467363253No Hit
CTCCTTCCAGGTGCTTTTGTGTGGAGTGTGTGGACCTCTTGGTGGGGCCG630.1982753194435702No Hit
CTTCCTCTTCTCAGCGGGACAGGTGGGTAAACCTTTGGAGGGTCAAATTC550.17309750110152955No Hit
CTCCTCTTCTCAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCAAATTC530.16680304651601938No Hit
CTTCCTCTTCTCAGCTGAGACAGGTGGGTAAACCTTTGGAGGGTCAAATT510.1605085919305092No Hit
CTTCCTCTTCTCAGCTGGGCAGGTGGGTAAACCTTTGGAGGGTCAAATTC430.13533077358846857No Hit
CTTCCTCTTCTCAGCTGGGACGGGTGGGTAAACCTTTGGAGGGTCAAATT430.13533077358846857No Hit
CTTCCTCTTCCAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCAAATTC370.11644740983193806No Hit
CTTCCTCTTCTCAGCAGTTGTTGTTCCGGGATTGTGTAGAACCATGTCGT370.11644740983193806No Hit
CTTCCTCTTCTCAGCTGGGACAGGTGGGTAAACCTTTGGGGGGTCAAATT320.10071127336816264No Hit
CTTCCTCTTCTCAGTTGGGACAGGTGGGTAAACCTTTGGAGGGTCAAATT320.10071127336816264No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)