FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008143828

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008143828
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7477
Total Bases1 Mbp
Sequences flagged as poor quality0
Sequence length142-145
%GC66

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGC550473.6124113949445No Hit
CGGAAACAACAACTGCTGAGAAGAGGAAGGGAAGTAGACCAAGTCTCTGC6608.827069680353084No Hit
CGGAAACAACAACTGCTGCGAAGAGGAAGGGAAGTAGACCAAGTCTCTGC1081.4444295840577772No Hit
CGGAAACAACAACAGCTGAGAAGAGGAAGGGAAGTAGACCAAGTCTCTGC781.0431991440417279No Hit
ACACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT460.6152200080246087No Hit
AAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT410.5483482680219339No Hit
ACAAAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT390.5215995720208639No Hit
ACAACAGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT340.45472783201818917No Hit
ACAACAGGTCAGATGCACCTGGAAGGAGGGAAGTAGACCAAGTCTCTGCT280.37448174401497925No Hit
ACAACAGGTCAGAAGCACCTGGAAGGAGGGAAGTAGACCAAGTCTCTGCT280.37448174401497925No Hit
ACAACAGGTCGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT250.33435870001337437No Hit
ACAACAGGTCAGATGCAGGCGCTGCCCATGGGAGCCCTGCCCCAGGGGCC230.30761000401230437No Hit
ACAACAGGTCAGATCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT220.29423565601176943No Hit
CAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT200.2674869600106995No Hit
ACAACAGGTCAGAGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT180.24073826400962955No Hit
ACAACAGGCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT180.24073826400962955No Hit
ACAACAGGTCAGATGCAGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT170.22736391600909459No Hit
ACAACAGGTCAGATGCAGGCCCATCGGTGCCCCAAGCTCCCACCCAGCGC160.21398956800855956No Hit
ACAACAGGTAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT150.2006152200080246No Hit
ACAACGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT130.17386652400695465No Hit
ACAACAGGTCAGATGCAGGCCCATCGGCGTCCCAAGCTCCCACCCAGCGC130.17386652400695465No Hit
ACAACAGGTCAATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT120.16049217600641968No Hit
ACAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCCCCCACCCAGCGC120.16049217600641968No Hit
ACAACAGGTCAGTGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT120.16049217600641968No Hit
ACAACAGGTCAGATGCAGGCCCATCGGTGTCCCGAGCTCCCACCCAGCGC110.14711782800588472No Hit
CGGAAACAACAACTGCTGCGGCGGCGAGAGGACTGGCCCTCCCGGCTCCA100.13374348000534975No Hit
CGGAACAACAACTGCTGAGAAGAGGAAGGGAAGTAGACCAAGTCTCTGCT100.13374348000534975No Hit
ACAACAGGTCAGATGCGGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGC100.13374348000534975No Hit
ACAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGTTCCCACCCAGCGC90.12036913200481478No Hit
ACAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCGGCGC80.10699478400427978No Hit
ACAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCGCCCAGCGC80.10699478400427978No Hit
CGGAAACAACACTGCTGAGAAGAGGAAGGGAAGTAGACCAAGTCTCTGCT80.10699478400427978No Hit
ACAACAGGTCAGATGCAGGCCCATCGGTGTCCCAGGCTCCCACCCAGCGC80.10699478400427978No Hit
ACAACAGGTCAGATGCAGGCCCATTGGTGTCCCAAGCTCCCACCCAGCGC80.10699478400427978No Hit
ACAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACTCAGCGC80.10699478400427978No Hit
ACAACAGGTCAGATGCAGGCCCATCGGTGTTCCAAGCTCCCACCCAGCGC80.10699478400427978No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)