FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008143870

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008143870
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2065
Total Bases299.4 kbp
Sequences flagged as poor quality0
Sequence length144-145
%GC60

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAACAACAACTGCTGAGAAGAGGAAGGGAAGTAGACCAAGTCTCTGC140067.79661016949152No Hit
ACAACAGGTCAGATGCACCTGGAAGGAGGGAAGTAGACCAAGTCTCTGCT844.067796610169491No Hit
CGGAAACAACAACAGCTGAGAAGAGGAAGGGAAGTAGACCAAGTCTCTGC783.777239709443099No Hit
CGGAAACAACAACTGCTGCGAAGAGGAAGGGAAGTAGACCAAGTCTCTGC743.583535108958838No Hit
ACAACAGGTCAGAAGCACCTGGAAGGAGGGAAGTAGACCAAGTCTCTGCT623.002421307506053No Hit
CGGAACAACAACTGCTGAGAAGAGGAAGGGAAGTAGACCAAGTCTCTGCT170.8232445520581114No Hit
CGAAACAACAACTGCTGAGAAGAGGAAGGGAAGTAGACCAAGTCTCTGCT170.8232445520581114No Hit
CGGAAACAACACTGCTGAGAAGAGGAAGGGAAGTAGACCAAGTCTCTGCT140.6779661016949152No Hit
CGGAAACACAACTGCTGAGAAGAGGAAGGGAAGTAGACCAAGTCTCTGCT130.6295399515738499No Hit
CGGAAACAACAACTGCTGAGAAGAGAAGGGAAGTAGACCAAGTCTCTGCT130.6295399515738499No Hit
CGGAAAAACAACTGCTGAGAAGAGGAAGGGAAGTAGACCAAGTCTCTGCT130.6295399515738499No Hit
ACAACAGGTCAAAAGCACCTGGAAGGAGGGAAGTAGACCAAGTCTCTGCT110.5326876513317191No Hit
CGGAAACAACAACTGCTGAGAAGAGGAAGGAAGTAGACCAAGTCTCTGCT100.48426150121065376No Hit
GGAAACAACAACTGCTGAGAAGAGGAAGGGAAGTAGACCAAGTCTCTGCT100.48426150121065376No Hit
CGGAAACAAAACTGCTGAGAAGAGGAAGGGAAGTAGACCAAGTCTCTGCT80.387409200968523No Hit
CGGAAACAACAATGCTGAGAAGAGGAAGGGAAGTAGACCAAGTCTCTGCT60.29055690072639223No Hit
CGGAAACAACAACGCTGAGAAGAGGAAGGGAAGTAGACCAAGTCTCTGCT60.29055690072639223No Hit
CGGAAACAACAACTCTGAGAAGAGGAAGGGAAGTAGACCAAGTCTCTGCT60.29055690072639223No Hit
CGGAAACAACAACTGCTGAGAAGGGAAGGGAAGTAGACCAAGTCTCTGCT50.24213075060532688No Hit
CGGAAACAACAACTACTGAGAAGAGGAAGGGAAGTAGACCAAGTCTCTGC50.24213075060532688No Hit
CGGAAACAACAACTGCTGAGAGAGGAAGGGAAGTAGACCAAGTCTCTGCT40.1937046004842615No Hit
CGGAAACAACAACTGCTGAGAAGAGGAAGGGAAGTAGACCAAGTCTCAGC40.1937046004842615No Hit
CGGAAACAACAACTGTTGAGAAGAGGAAGGGAAGTAGACCAAGTCTCTGC40.1937046004842615No Hit
CGGAAACAACAACTGCTGAGAAGAGGAGGGAAGTAGACCAAGTCTCTGCT40.1937046004842615No Hit
CGGAAACAACAACTGAGAAGAGGAAGGGAAGTAGACCAAGTCTCTGCTAC40.1937046004842615No Hit
CGGAAACAACAACTGCTGGGAAGAGGAAGGGAAGTAGACCAAGTCTCTGC40.1937046004842615No Hit
ACAACAGGTCAATGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT40.1937046004842615Clontech Universal Primer Mix Long (100% over 23bp)
ACAACAGGTCAAAGCACCTGGAAGGAGGGAAGTAGACCAAGTCTCTGCTA30.14527845036319612No Hit
CGGAAACAACAACTGCTGAGAAGAGGAAGGGAAGTAGACCAACTCTCTGC30.14527845036319612No Hit
CGGAAACAACAACTGCTGAAAGAGGAAGGGAAGTAGACCAAGTCTCTGCT30.14527845036319612No Hit
ACAACAGGTCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT30.14527845036319612Clontech Universal Primer Mix Long (100% over 23bp)
CGGAAACAACAACTGCTGAGAAGAGGAAGGGAAGTAGACCAAGACTCTGC30.14527845036319612No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)