FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008143883

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008143883
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16054
Total Bases2.3 Mbp
Sequences flagged as poor quality0
Sequence length142-145
%GC62

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTTCCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGCCG738746.0134545907562No Hit
CTTCCTCTTCTCAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCAAATT392824.467422449233837No Hit
CTTCCTCTTCTCAGCAGTTGTTGTTTCCGCGTACTTGTAGAACCATGTCG163510.184377725177526No Hit
CTTCCTCTTCTCAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCCTAAG5173.220381213404759No Hit
CTTCCTCTTCGCAGCAGTTGTTGTTTCCGCGTACTTGTAGAACCATGTCG1330.8284539678584776No Hit
CTCCTTCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGCCGG1040.6478136290021179No Hit
CTCTTCCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGCCGG960.5979818113865703No Hit
CTTCCTCTTCTCAGCTGTTGTTGTTTCCGCGTACTTGTAGAACCATGTCG940.5855238569826835No Hit
CTCCTCCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGCCGG510.3176778372991155No Hit
TCCTTCCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGCCGG440.2740749968855114No Hit
CTTCCTCTTCTCAGCAGTTGTTGTTCCGCGTACTTGTAGAACCATGTCGT400.24915908807773765No Hit
CCCTTCCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGCCGG370.23047215647190733No Hit
CTTCCTCTTCTAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCAAATTC350.21801420206802044No Hit
CTCCTTCCAGTGCTTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGCCGG340.21178522486607698No Hit
CTCCTTCCAGGTGCTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGCCGG330.20555624766413358No Hit
CTTCCTCTCTCAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCAAATTC320.19932727046219012No Hit
CTCCTTCCGGTGCTTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGCCGG300.1868693160583032No Hit
CTTCTCTTCTCAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCAAATTC290.18064033885635977No Hit
CTCCTTCCAGGTGCTTTTGTGTGGAGTGTGTGGACCTCTTGGTGGGGCCG280.17441136165441634No Hit
CTTCCTCTTTCAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCAAATTC280.17441136165441634No Hit
CTTCCTTTCTCAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCAAATTC260.16195340725052948No Hit
CTTCCTCTTCTCAGTGGGACAGGTGGGTAAACCTTTGGAGGGTCAAATTC210.13080852124081227No Hit
CTCCTTCCAGGTGCATCTGACCTGTTGTCGTACTTGTAGAACCATGTCGT210.13080852124081227No Hit
CTTCCTCTTCTCGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCAAATTC200.12457954403886883No Hit
CTCCTTCCAGGTGCTTTTGCGTGGAGTGTGTGGGCCTCTTGGTGGGGCCG190.11835056683692537No Hit
CTTCCTCTTCTCAGCAGTTGTTTTTCCGCGTACTTGTAGAACCATGTCGT180.11212158963498194No Hit
CTCCTTCCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTGGGTGGGGCCG180.11212158963498194No Hit
CTCCTTCCAGGTGCTTTTGCGTGGAGTGTGCGGACCTCTTGGTGGGGCCG170.10589261243303849No Hit
CTCCTTCCAGGTGCCTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGCCG170.10589261243303849No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)