FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008143957

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008143957
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13440
Total Bases1.9 Mbp
Sequences flagged as poor quality0
Sequence length142-145
%GC62

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTTCCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGCCG554841.279761904761905No Hit
CTTCCTCTTCTCAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCAAATT339225.238095238095237No Hit
CTTCCTCTTCTCAGCAGTTGTTGTTTCCGCGTACTTGTAGAACCATGTCG12329.166666666666666No Hit
CTTCCTCTTCTCAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCCTAAG9116.778273809523809No Hit
CTTCCTCTTCGCAGCAGTTGTTGTTTCCGCGTACTTGTAGAACCATGTCG1431.0639880952380953No Hit
CTTCCTCTTCTCAGCTGTTGTTGTTTCCGCGTACTTGTAGAACCATGTCG1090.8110119047619049No Hit
CTCCTTCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGCCGG730.5431547619047619No Hit
CTCTTCCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGCCGG690.5133928571428571No Hit
TCCTTCCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGCCGG410.30505952380952384No Hit
CTCCTCCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGCCGG370.27529761904761907No Hit
CTCCTTCCAGGTGCTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGCCGG340.25297619047619047No Hit
CTCCTTCCAGGTGCATCTGACCTGTTGTCGTACTTGTAGAACCATGTCGT330.24553571428571427No Hit
CTTCCTCTTCTCGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCAAATTC320.2380952380952381No Hit
CTTCCTCTTCTAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCAAATTC320.2380952380952381No Hit
CTTCCTCTTCTCAGCAGTTGTTGTTCCGCGTACTTGTAGAACCATGTCGT290.21577380952380953No Hit
CCCTTCCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGCCGG270.20089285714285712No Hit
CTTCCTTTCTCAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCAAATTC270.20089285714285712No Hit
CTCCTTCCAGTGCTTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGCCGG260.19345238095238096No Hit
CTTCCTCTTCTCACTGGGACAGGTGGGTAAACCTTTGGAGGGTCAAATTC240.17857142857142858No Hit
CTTCTCTTCTCAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCAAATTC240.17857142857142858No Hit
CTTCCTCTTTCAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCAAATTC230.17113095238095238No Hit
CTTCCTCTCTCAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCAAATTC220.1636904761904762No Hit
CTTCCTCTTCTCAGTGGGACAGGTGGGTAAACCTTTGGAGGGTCAAATTC210.15625No Hit
CTCCTTCCAGGTGCTTCTGACCTGTTGTCGTACTTGTAGAACCATGTCGT200.1488095238095238No Hit
CTCCTTCCGGTGCTTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGCCGG200.1488095238095238No Hit
CTTCCTCTTCCCAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCAAATT190.14136904761904762No Hit
CTCCTTCCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGCCC180.13392857142857142No Hit
CTTCCTCTTCTCAGCTGAGACAGGTGGGTAAACCTTTGGAGGGTCAAATT170.12648809523809523No Hit
CTTCCTCTTCTCAGCTGGGACGGGTGGGTAAACCTTTGGAGGGTCAAATT160.11904761904761905No Hit
CTCCTTCCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGCTG150.11160714285714285No Hit
CTCCTTCCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGCCA150.11160714285714285No Hit
CTCCTCTTCTCAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCAAATTC150.11160714285714285No Hit
CTCCTTCCAGGTGCTTTTGCGTGGAGTGTGTGGACCCCTTGGTGGGGCCG140.10416666666666667No Hit
CTCCTTCCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGTCG140.10416666666666667No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)