FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008143981

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008143981
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8502
Total Bases1.2 Mbp
Sequences flagged as poor quality0
Sequence length135-145
%GC64

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTTCCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGCCG601070.68924958833216No Hit
CTTCCTCTTCTCAGCAGTTGTTGTTTCCGCGTACTTGTAGAACCATGTCG102412.044224888261585No Hit
CTCTTCCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGCCGG1021.1997177134791814No Hit
CTTCCTCTTCGCAGCAGTTGTTGTTTCCGCGTACTTGTAGAACCATGTCG1011.1879557751117384No Hit
CTTCCTCTTCTCAGCTGTTGTTGTTTCCGCGTACTTGTAGAACCATGTCG891.046812514702423No Hit
CTCCTTCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGCCGG730.8586215008233357No Hit
CTTCCTCTTCTCAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCCTAAG680.7998118089861209No Hit
CTCCTCCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGCCGG480.5645730416372619No Hit
TCCTTCCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGCCGG370.4351917195953893No Hit
CTCCTTCCAGGTGCTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGCCGG280.32933427428840273No Hit
CTTCCTCTTCTCAGCAGTTGTTGTTCCGCGTACTTGTAGAACCATGTCGT240.2822865208186309No Hit
CTCCTTCCAGTGCTTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGCCGG210.24700070571630206No Hit
CTCCTTCCGGTGCTTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGCCGG210.24700070571630206No Hit
CTCCTTCCAGGTGCATCTGACCTGTTGTCGTACTTGTAGAACCATGTCGT210.24700070571630206No Hit
CCCTTCCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGCCGG200.2352387673488591No Hit
CTCCTTCCAGGTGCTTTTGCGTGGAGTGTGTGGACCCCTTGGTGGGGCCG150.17642907551164433No Hit
CTCCTTCCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGACCG140.16466713714420136No Hit
CTCCTTCCAGGTGCTTTTGCGTGGAGTGTGTGGGCCTCTTGGTGGGGCCG140.16466713714420136No Hit
CTCCTTCCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGCTG130.1529051987767584No Hit
CTCCTTCCAGGTGCTTTTGCGTGGAGCGTGTGGACCTCTTGGTGGGGCCG120.14114326040931546No Hit
CTCCTTCCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGTCG120.14114326040931546No Hit
CTCCTTCCAGGTGCTTTTGTGTGGAGTGTGTGGACCTCTTGGTGGGGCCG110.1293813220418725No Hit
CTCCTTCCAGGTGCTTCTGACCTGTTGTCGTACTTGTAGAACCATGTCGT100.11761938367442955No Hit
CTCCTTCCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTTGGCGGGGCCG100.11761938367442955No Hit
CTTCTCTTCTCAGCAGTTGTTGTTTCCGCGTACTTGTAGAACCATGTCGT100.11761938367442955No Hit
CTCCTTCCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGCCT100.11761938367442955No Hit
CTCCTTCCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGCCA100.11761938367442955No Hit
CTTCCTCTTCTCAGCAGTTGTGTTTCCGCGTACTTGTAGAACCATGTCGT100.11761938367442955No Hit
CTCCTTCCAGGTGCTTTTGCGTGGAGTGTGCGGACCTCTTGGTGGGGCCG90.1058574453069866No Hit
CTCCTTCCAGGTGCCTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGCCG90.1058574453069866No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)