FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008143994

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008143994
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1927
Total Bases279.3 kbp
Sequences flagged as poor quality0
Sequence length143-145
%GC59

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAACAACAACTGCTGAGAAGAGGAAGCAAGGAAGACCAAGTCTCTGC138671.9252724442138No Hit
ACAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGC884.566683964711987No Hit
CGGAAACAACAACAGCTGAGAAGAGGAAGCAAGGAAGACCAAGTCTCTGC703.632589517384536No Hit
CGGAAACAACAACTGCTGCGAAGAGGAAGCAAGGAAGACCAAGTCTCTGC502.5947067981318113No Hit
ACAACAGGTCAGATGCACCTGGAAGGAGCAAGGAAGACCAAGTCTCTGCT402.075765438505449No Hit
ACAACAGGTCAGAAGCACCTGGAAGGAGCAAGGAAGACCAAGTCTCTGCT261.3492475350285418No Hit
CGGAACAACAACTGCTGAGAAGAGGAAGCAAGGAAGACCAAGTCTCTGCT251.2973533990659056No Hit
CGAAACAACAACTGCTGAGAAGAGGAAGCAAGGAAGACCAAGTCTCTGCT160.8303061754021795No Hit
CGGAAAAACAACTGCTGAGAAGAGGAAGCAAGGAAGACCAAGTCTCTGCT110.5708354955889984No Hit
CGGAAACACAACTGCTGAGAAGAGGAAGCAAGGAAGACCAAGTCTCTGCT110.5708354955889984No Hit
CGGAAACAACAACTGCTGAGAAGAGAAGCAAGGAAGACCAAGTCTCTGCT110.5708354955889984No Hit
CGGAAACAACACTGCTGAGAAGAGGAAGCAAGGAAGACCAAGTCTCTGCT80.4151530877010898No Hit
CGGAAACAACAACTGCTGAGAAAGGAAGCAAGGAAGACCAAGTCTCTGCT70.3632589517384536No Hit
CGGAAACAAAACTGCTGAGAAGAGGAAGCAAGGAAGACCAAGTCTCTGCT70.3632589517384536No Hit
GGAAACAACAACTGCTGAGAAGAGGAAGCAAGGAAGACCAAGTCTCTGCT70.3632589517384536No Hit
CGGAAACAACAACTCTGAGAAGAGGAAGCAAGGAAGACCAAGTCTCTGCT60.3113648157758173No Hit
CGGAAACAACAACTACTGAGAAGAGGAAGCAAGGAAGACCAAGTCTCTGC50.2594706798131811No Hit
CGGAAACAACAACTGAGAAGAGGAAGCAAGGAAGACCAAGTCTCTGCTAC40.2075765438505449No Hit
CGGAAACAACAACCGCTGAGAAGAGGAAGCAAGGAAGACCAAGTCTCTGC40.2075765438505449No Hit
CGGAAACAACAACTGCTGAAAGAGGAAGCAAGGAAGACCAAGTCTCTGCT40.2075765438505449No Hit
CGGAAACAACAACTGCTGAGAGAGGAAGCAAGGAAGACCAAGTCTCTGCT40.2075765438505449No Hit
CGGAACAACAACTGCTGCGAAGAGGAAGCAAGGAAGACCAAGTCTCTGCT30.15568240788790866No Hit
ACAACAGGTCAATGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT30.15568240788790866Clontech Universal Primer Mix Long (100% over 23bp)
CGGAAACAACAACTGCTGAGAAGAGGAGCAAGGAAGACCAAGTCTCTGCT30.15568240788790866No Hit
CGGAAACAACAACTGCCGAGAAGAGGAAGCAAGGAAGACCAAGTCTCTGC30.15568240788790866No Hit
CGGAAACAACAACTGCTGAGAAGAAGAAGCAAGGAAGACCAAGTCTCTGC20.10378827192527244No Hit
CGGAAACAACAACTGCTGAGAAGAGGAGGCAAGGAAGACCAAGTCTCTGC20.10378827192527244No Hit
CGGAAACAACAACTGCTGAGAAGAGGAAGCAAGGAAGACAAAGTCTCTGC20.10378827192527244No Hit
CGGAAACAACAACTGCTGAGAAGATGAAGCAAGGAAGACCAAGTCTCTGC20.10378827192527244No Hit
CGGAAACAACAACGCTGAGAAGAGGAAGCAAGGAAGACCAAGTCTCTGCT20.10378827192527244No Hit
ACAACAGGTCAAAAGCACCTGGAAGGAGCAAGGAAGACCAAGTCTCTGCT20.10378827192527244No Hit
CGGAACCAACAACTGCTGAGAAGAGGAAGCAAGGAAGACCAAGTCTCTGC20.10378827192527244No Hit
CGGAAACAACAACTGCTGGGAAGAGGAAGCAAGGAAGACCAAGTCTCTGC20.10378827192527244No Hit
ACAACAGGTCAGATGCACCTGGAAGAGCAAGGAAGACCAAGTCTCTGCTA20.10378827192527244No Hit
CGGAAACAACAACTGCTGAGAAGAGGAAGCAAGGAAGACCAAGTCTCTGA20.10378827192527244No Hit
ACAACAGGTCAATGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTA20.10378827192527244Clontech Universal Primer Mix Long (96% over 25bp)
CGGAAACAACAACTGCTGAGAAGGGAAGCAAGGAAGACCAAGTCTCTGCT20.10378827192527244No Hit
CCGGAAACAACAACTGCTGAGAAGAGGAAGCAAGGAAGACCAAGTCTCTG20.10378827192527244No Hit
CAGAAACAACAACTGCTGAGAAGAGGAAGCAAGGAAGACCAAGTCTCTGC20.10378827192527244No Hit
CGAACAACAACTGCTGAGAAGAGGAAGCAAGGAAGACCAAGTCTCTGCTA20.10378827192527244No Hit
CGGAACAACAACAGCTGAGAAGAGGAAGCAAGGAAGACCAAGTCTCTGCT20.10378827192527244No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)