FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008143995

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008143995
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1927
Total Bases279.4 kbp
Sequences flagged as poor quality0
Sequence length143-145
%GC63

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCCTCTTCTCAGCAGTTGTTGTTTCCGCGTACTTGTAGAACCATGTCG135970.52413077322262No Hit
CTCCTTCCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGCCG784.0477426050856256No Hit
CTTCCTCTTCTCAGCTGTTGTTGTTTCCGCGTACTTGTAGAACCATGTCG683.5288012454592628No Hit
CTTCCTCTTCGCAGCAGTTGTTGTTTCCGCGTACTTGTAGAACCATGTCG522.6984950700570836No Hit
CTCCTTCCAGGTGCATCTGACCTGTTGTCGTACTTGTAGAACCATGTCGT402.075765438505449No Hit
CTCCTTCCAGGTGCTTCTGACCTGTTGTCGTACTTGTAGAACCATGTCGT271.401141670991178No Hit
CTTCCTCTTCTCAGCAGTTGTTGTTCCGCGTACTTGTAGAACCATGTCGT261.3492475350285418No Hit
CTTCCTCTTCTCAGCAGTTGTTGTTTCGCGTACTTGTAGAACCATGTCGT170.8822003113648157No Hit
CTTCCTCTTCTCAGCAGTTGTTTTTCCGCGTACTTGTAGAACCATGTCGT110.5708354955889984No Hit
CTTCTCTTCTCAGCAGTTGTTGTTTCCGCGTACTTGTAGAACCATGTCGT110.5708354955889984No Hit
CTTCCTCTTCTCAGCAGTTGTGTTTCCGCGTACTTGTAGAACCATGTCGT100.5189413596263622No Hit
CTTCCTCTTCTCAGCAGTGTTGTTTCCGCGTACTTGTAGAACCATGTCGT80.4151530877010898No Hit
CTTCCTTTCTCAGCAGTTGTTGTTTCCGCGTACTTGTAGAACCATGTCGT70.3632589517384536No Hit
CTTCCTCTTCTCAGCAGTTTTGTTTCCGCGTACTTGTAGAACCATGTCGT70.3632589517384536No Hit
CTTCCTCTTCTCAGCAGTTGTTGTTTCCCGTACTTGTAGAACCATGTCGT60.3113648157758173No Hit
CTCTTCCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGCCGG50.2594706798131811No Hit
CTTCCTCTTCTCAGTAGTTGTTGTTTCCGCGTACTTGTAGAACCATGTCG50.2594706798131811No Hit
CTCCTTCCAGGTGCTTTTGCGTTGATACCACTGCTTCCCATGTACTCTGC50.2594706798131811Clontech Universal Primer Mix Long (95% over 21bp)
CTTCCTCTTCTCAGCGGTTGTTGTTTCCGCGTACTTGTAGAACCATGTCG40.2075765438505449No Hit
CTTCCTCTTCTCAGAGTTGTTGTTTCCGCGTACTTGTAGAACCATGTCGT40.2075765438505449No Hit
CTTCCTCTTCTCAGTTGTTGTTTCCGCGTACTTGTAGAACCATGTCGTCA40.2075765438505449No Hit
CTTCCTCTTTCAGCAGTTGTTGTTTCCGCGTACTTGTAGAACCATGTCGT40.2075765438505449No Hit
CTTCCTCTTCTCAGCAGTTGTTGTTTCAGCGTACTTGTAGAACCATGTCG40.2075765438505449No Hit
CTTCCTCTTCTCGGCAGTTGTTGTTTCCGCGTACTTGTAGAACCATGTCG30.15568240788790866No Hit
CTTCCTCTTCTCAGCAGTTGTTGTTTCCGCGTACTTGTAGAACCATGACG30.15568240788790866No Hit
CTCCTCTTCTCAGCAGTTGTTGTTTCCGCGTACTTGTAGAACCATGTCGT30.15568240788790866No Hit
CTTCCTCTCTCAGCAGTTGTTGTTTCCGCGTACTTGTAGAACCATGTCGT30.15568240788790866No Hit
CTCCTTCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGCCGG30.15568240788790866No Hit
CTTCCTCTTCTCAGCAGTTGTTGTTTCCGCGTACTTGTAGAACCATGTCC30.15568240788790866No Hit
CTTCCTCTTCGCAGCAGTTGTTGTTCCGCGTACTTGTAGAACCATGTCGT30.15568240788790866No Hit
CTCTTCCAGGTGCATCTGACCTGTTGTCGTACTTGTAGAACCATGTCGTC20.10378827192527244No Hit
CTCCTTCCAGGTGCTTTTGCGTGGGGTGTGTGGACCTCTTGGTGGGGCCG20.10378827192527244No Hit
CTTCCTCTTCTCAGCAGTTGTTGTTTCCGCGTACTTGTAGAACCAAGTCG20.10378827192527244No Hit
CTTCCTCTTCTCAGCAGTTGTTGTTCGCGTACTTGTAGAACCATGTCGTC20.10378827192527244No Hit
CTTCCTCTTCTCAGCAGTTGTTGGTTCCGCGTACTTGTAGAACCATGTCG20.10378827192527244No Hit
CTTCCCTTCTCAGCAGTTGTTGTTTCCGCGTACTTGTAGAACCATGTCGT20.10378827192527244No Hit
CTTCCTCTTCTCAGCTGTTGTTGTTCCGCGTACTTGTAGAACCATGTCGT20.10378827192527244No Hit
CTTCCTCTTCTCAGCAGTTGTTGTTTCCGCGTACTTGTAGAACCAGGTCG20.10378827192527244No Hit
CTTCCTCTTCTCAGCAGTTGTTGTTTCCGGCGTACTTGTAGAACCATGTC20.10378827192527244No Hit
CTTCCTCTTCCCAGCAGTTGTTGTTTCCGCGTACTTGTAGAACCATGTCG20.10378827192527244No Hit
CCTCCTCTTCTCAGCAGTTGTTGTTTCCGCGTACTTGTAGAACCATGTCG20.10378827192527244No Hit
CTTCCTCTTCTCAGCAGATGTTGTTTCCGCGTACTTGTAGAACCATGTCG20.10378827192527244No Hit
CTCCTTCCAGGTGCTTTTGACCTGTTGTCGTACTTGTAGAACCATGTCGT20.10378827192527244No Hit
CTCCTTCCAGGTGCTTTGCGTTGATACCACTGCTTCCCATGTACTCTGCG20.10378827192527244Clontech Universal Primer Mix Long (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)