FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008144029

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008144029
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16508
Total Bases2.3 Mbp
Sequences flagged as poor quality0
Sequence length143-145
%GC62

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTTCCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGCCG620837.606009207656896No Hit
CTTCCTCTTCTCAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCAAATT509730.87593893869639No Hit
CTTCCTCTTCTCAGCAGTTGTTGTTTCCGCGTACTTGTAGAACCATGTCG188411.41264841289072No Hit
CTTCCTCTTCTCAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCCTAAG4782.8955657862854376No Hit
CTTCCTCTTCGCAGCAGTTGTTGTTTCCGCGTACTTGTAGAACCATGTCG1520.9207656893627332No Hit
CTTCCTCTTCTCAGCTGTTGTTGTTTCCGCGTACTTGTAGAACCATGTCG1360.8238429852192876No Hit
CTCTTCCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGCCGG990.5997092318875696No Hit
CTCCTTCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGCCGG710.43009449963653984No Hit
CTTCCTCTTCTAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCAAATTC580.3513448025199903No Hit
CTCCTCCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGCCGG510.30894111945723285No Hit
CTTCCTCTTCTCAGCAGTTGTTGTTCCGCGTACTTGTAGAACCATGTCGT480.2907681124303368No Hit
CTTCCTCTTTCAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCAAATTC470.2847104434213715No Hit
CTTCCTTTCTCAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCAAATTC460.2786527744124061No Hit
CTCCTTCCAGGTGCATCTGACCTGTTGTCGTACTTGTAGAACCATGTCGT460.2786527744124061No Hit
CTCCTTCCAGGTGCTTCTGACCTGTTGTCGTACTTGTAGAACCATGTCGT440.2665374363944754No Hit
CTTCCTCTTCTCGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCAAATTC370.22413375333171795No Hit
CTTCCTCTTCTCAGCTGAGACAGGTGGGTAAACCTTTGGAGGGTCAAATT370.22413375333171795No Hit
CTTCCTCTTCTCACTGGGACAGGTGGGTAAACCTTTGGAGGGTCAAATTC360.21807608432275258No Hit
TCCTTCCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGCCGG350.21201841531378723No Hit
CTTCCTCTTCTCAGTGGGACAGGTGGGTAAACCTTTGGAGGGTCAAATTC340.2059607463048219No Hit
CTTCCTCTCTCAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCAAATTC320.1938454082868912No Hit
CTCCTTCCAGGTGCTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGCCGG310.18778773927792586No Hit
CCCTTCCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGCCGG290.17567240125999514No Hit
CTTCTCTTCTCAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCAAATTC260.1574993942330991No Hit
CTCCTTCCGGTGCTTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGCCGG260.1574993942330991No Hit
CTTCCTCTTCCCAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCAAATT230.13932638720620305No Hit
CTTCCTCTTCCAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCAAATTC230.13932638720620305No Hit
CTCCTTCCAGTGCTTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGCCGG190.11509571117034165No Hit
CTCCTTCCAGGTGCTTTTGCGTGGAGTGTGCGGACCTCTTGGTGGGGCCG170.10298037315241095No Hit
CTTCCTCTTCTCAGCTGGGACGGGTGGGTAAACCTTTGGAGGGTCAAATT170.10298037315241095No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)