FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008144050

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008144050
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9037
Total Bases1.3 Mbp
Sequences flagged as poor quality0
Sequence length143-145
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGC636370.41053446940356No Hit
CGGAAACAACAACTGCTGAGAAGAGGAAGCAAGGAAGACCAAGTCTCTGC100811.15414407436096No Hit
CGGAAACAACAACTGCTGCGGCGGCGAGAGGACTGGCCCTCCCGGCTCCA1571.7373022020582052No Hit
CGGAAACAACAACTGCTGCGAAGAGGAAGCAAGGAAGACCAAGTCTCTGC1281.4163992475378997No Hit
CGGAAACAACAACAGCTGAGAAGAGGAAGCAAGGAAGACCAAGTCTCTGC1081.195086865110103No Hit
ACACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT570.6307402899192209No Hit
ACAAAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT490.5422153369481022No Hit
ACAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGTGC490.5422153369481022No Hit
AAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT350.3872966692486445No Hit
ACAACAGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT290.32090295452030543No Hit
ACAACGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT270.29877171627752575No Hit
ACAACAGGTAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT260.2877060971561359No Hit
ACAACAGGCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT250.276640478034746No Hit
ACAACAGGTCGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT220.2434436206705765No Hit
CGGAACAACAACTGCTGAGAAGAGGAAGCAAGGAAGACCAAGTCTCTGCT210.23237800154918667No Hit
ACAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCCCCCACCCAGCGC200.22131238242779683No Hit
ACAACAGGTCAATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT190.21024676330640701No Hit
CAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT190.21024676330640701No Hit
ACAACAGGTCAGATGCACCTGGAAGGAGCAAGGAAGACCAAGTCTCTGCT190.21024676330640701No Hit
ACAACAGGTCAGATCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT190.21024676330640701No Hit
ACAACAGGTCAGAAGCACCTGGAAGGAGCAAGGAAGACCAAGTCTCTGCT170.1881155250636273No Hit
ACAACAGGTCAGAGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT170.1881155250636273No Hit
CGGAAACAACAACTGCTGCAAGCTTGCCTGTCTCCACATACTGCTTCTGC150.16598428682084762No Hit
ACAACAGGTCAGATGCAGGTCCATCGGTGTCCCAAGCTCCCACCCAGCGC140.1549186676994578No Hit
ACAACAGGTCAGGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCG140.1549186676994578No Hit
ACAACAGGTCAGATGCAGGCCCATCGGTGTTCCAAGCTCCCACCCAGCGC140.1549186676994578No Hit
CGGAAAAACAACTGCTGAGAAGAGGAAGCAAGGAAGACCAAGTCTCTGCT130.14385304857806794No Hit
ACAACAGGTCAGATGCAGGCCCATCAGTGTCCCAAGCTCCCACCCAGCGC120.1327874294566781No Hit
ACAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACTCAGCGC120.1327874294566781No Hit
ACAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCGCCCAGCGC110.12172181033528826No Hit
ACAACAGGTCAGATGCAGGCCCGTCGGTGTCCCAAGCTCCCACCCAGCGC110.12172181033528826No Hit
ACAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCGGCGC100.11065619121389841No Hit
ACAACAGGTCAGATGCAGGCCCATCGGCGTCCCAAGCTCCCACCCAGCGC100.11065619121389841No Hit
ACAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCTCACCCAGCGC100.11065619121389841No Hit
ACAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCACCCACCCAGCGC100.11065619121389841No Hit
ACAACAGGTCAGATGCAGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT100.11065619121389841No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)