FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008144147

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008144147
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14088
Total Bases2 Mbp
Sequences flagged as poor quality0
Sequence length136-145
%GC63

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTTCCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGCCG690649.02044293015332No Hit
CTTCCTCTTCTCAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCAAATT280719.92475865985236No Hit
CTTCCTCTTCTCAGCAGTTGTTGTTTCCGCGTACTTGTAGAACCATGTCG13689.710391822827939No Hit
CTTCCTCTTCTCAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCCTAAG5784.102782509937535No Hit
CTTCCTCTTCGCAGCAGTTGTTGTTTCCGCGTACTTGTAGAACCATGTCG1581.1215218625780807No Hit
CTTCCTCTTCTCAGCTGTTGTTGTTTCCGCGTACTTGTAGAACCATGTCG1320.9369676320272573No Hit
CTCTTCCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGCCGG1120.7950028392958546No Hit
CTCCTTCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGCCGG740.5252697331061896No Hit
CTCCTCCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGCCGG470.3336172629187961No Hit
TCCTTCCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGCCGG390.2768313458262351No Hit
CTCCTTCCAGGTGCTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGCCGG380.26973310618966495No Hit
CTCCTTCCGGTGCTTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGCCGG320.2271436683702442No Hit
CTCCTTCCAGGTGCTTTTGCGTGGAGTGTGCGGACCTCTTGGTGGGGCCG290.20584894946053378No Hit
CCCTTCCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGCCGG290.20584894946053378No Hit
CTCCTTCCAGTGCTTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGCCGG270.19165247018739354No Hit
CTCCTTCCAGGTGCTTTTGCGTGGAGTGTGTGGACCCCTTGGTGGGGCCG260.1845542305508234No Hit
CTTCCTCTTCTCAGCAGTTGTTGTTCCGCGTACTTGTAGAACCATGTCGT220.15616127200454286No Hit
CTCCTTCCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTGGGTGGGGCCG210.14906303236797275No Hit
CTTCCTCTTCTCAGTGGGACAGGTGGGTAAACCTTTGGAGGGTCAAATTC200.1419647927314026No Hit
CTTCCTCTCTCAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCAAATTC200.1419647927314026No Hit
CTTCCTCTTCTAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCAAATTC190.13486655309483248No Hit
CTCCTTCCAGGTGCTTTTGTGTGGAGTGTGTGGACCTCTTGGTGGGGCCG190.13486655309483248No Hit
CTCCTTCCAGGTGCTTTTGCGTGGAGTGTGTGGGCCTCTTGGTGGGGCCG190.13486655309483248No Hit
CTTCCTCTTCTCGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCAAATTC180.12776831345826234No Hit
CTTCCTTTCTCAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCAAATTC180.12776831345826234No Hit
CTCCTTCCAGGTGCATCTGACCTGTTGTCGTACTTGTAGAACCATGTCGT170.12067007382169223No Hit
CTCCTTCCAGGTGCTTTTGCGCGGAGTGTGTGGACCTCTTGGTGGGGCCG160.1135718341851221No Hit
CTCCTTCCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGACCG160.1135718341851221No Hit
CTTCTCTTCTCAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCAAATTC160.1135718341851221No Hit
CTCCTTCCAGGTGCTTTTGCGTGGAGTGCGTGGACCTCTTGGTGGGGCCG160.1135718341851221No Hit
CTCCTTCCAGGTGTTTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGCCG150.10647359454855196No Hit
CTCCTTCCAGGTGCTTTTGCGTGGAGCGTGTGGACCTCTTGGTGGGGCCG150.10647359454855196No Hit
CTCCTTCCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTTGGCGGGGCCG150.10647359454855196No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)