FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008144162

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008144162
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6142
Total Bases890.4 kbp
Sequences flagged as poor quality0
Sequence length143-145
%GC61

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAACAACAACTGCTGCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCT272944.43178117876913No Hit
CGGAAACAACAACTGCTGAGAAGAGGAAGCAAGGAAGACCAAGTCTCTGC136022.142624552263108No Hit
ACAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGC97015.79290133507001No Hit
CGGAAACAACAACAGCTGAGAAGAGGAAGCAAGGAAGACCAAGTCTCTGC881.4327580592640834No Hit
CGGAAACAACAACTGCTGCGAAGAGGAAGCAAGGAAGACCAAGTCTCTGC821.3350700097688049No Hit
CGGAACAACAACTGCTGCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTT600.9768804949527841No Hit
CGGAACAACAACTGCTGAGAAGAGGAAGCAAGGAAGACCAAGTCTCTGCT380.6186909801367633No Hit
CGGAAAAACAACTGCTGCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTT290.4721589058938457No Hit
ACAACAGGTCAGATGCACCTGGAAGGAGCAAGGAAGACCAAGTCTCTGCT270.4395962227287529No Hit
CGGAAACACAACTGCTGCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTT210.34190817323347444No Hit
CGAAACAACAACTGCTGCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTT210.34190817323347444No Hit
CGGAAAAACAACTGCTGAGAAGAGGAAGCAAGGAAGACCAAGTCTCTGCT160.2605014653207424No Hit
ACAACAGGTCAGAAGCACCTGGAAGGAGCAAGGAAGACCAAGTCTCTGCT150.24422012373819602No Hit
ACACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT150.24422012373819602No Hit
CGGAAACAACACTGCTGCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTT130.2116574405731032No Hit
CGGAAACAACAACTGCTGCAGGTGCTTTGCGTGGAGTGTGTGGACCTCTT120.19537609899055683No Hit
GGAAACAACAACTGCTGCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTT120.19537609899055683No Hit
AAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT100.16281341582546402No Hit
CGGAAACAACAACTGCTGCGGGTGCTTTTGCGTGGAGTGTGTGGACCTCT100.16281341582546402No Hit
ACAACAGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT100.16281341582546402No Hit
CGGAAACAACAACTGCTGCAGGTGCCTTTGCGTGGAGTGTGTGGACCTCT100.16281341582546402No Hit
CGGAAACAAAACTGCTGAGAAGAGGAAGCAAGGAAGACCAAGTCTCTGCT80.1302507326603712No Hit
CGGAAACAACAACTGCTGCAGGTGCTTTTGCGTGGAGTGTGTGGGCCTCT80.1302507326603712No Hit
CGAAACAACAACTGCTGAGAAGAGGAAGCAAGGAAGACCAAGTCTCTGCT80.1302507326603712No Hit
CGGAAACAACAACTGCTGAGAAGAGAAGCAAGGAAGACCAAGTCTCTGCT80.1302507326603712No Hit
ACAACAGGCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT80.1302507326603712No Hit
CGGAAACAAAACTGCTGCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTT70.11396939107782482No Hit
CGGAACCAACAACTGCTGAGAAGAGGAAGCAAGGAAGACCAAGTCTCTGC70.11396939107782482No Hit
CGGAAACAACAACTGCTGCAGGTGCTTTTGCATGGAGTGTGTGGACCTCT70.11396939107782482No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)