FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008144191

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008144191
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16593
Total Bases2.4 Mbp
Sequences flagged as poor quality0
Sequence length142-145
%GC62

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTTCCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGCCG722143.51835111191466No Hit
CTTCCTCTTCTCAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCAAATT383923.13626227927439No Hit
CTTCCTCTTCTCAGCAGTTGTTGTTTCCGCGTACTTGTAGAACCATGTCG205212.366660640028927No Hit
CTTCCTCTTCTCAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCCTAAG6664.013740734044476No Hit
CTTCCTCTTCTCAGCTGTTGTTGTTTCCGCGTACTTGTAGAACCATGTCG1270.7653829928283011No Hit
CTTCCTCTTCGCAGCAGTTGTTGTTTCCGCGTACTTGTAGAACCATGTCG1260.7593563550894956No Hit
CTCCTTCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGCCGG1040.6267703248357741No Hit
CTCTTCCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGCCGG960.57855722292533No Hit
CTCCTCCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGCCGG550.3314650756343036No Hit
CTTCCTCTTCTAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCAAATTC480.289278611462665No Hit
TCCTTCCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGCCGG460.27722533598505394No Hit
CTTCCTCTTCTCAGCAGTTGTTGTTCCGCGTACTTGTAGAACCATGTCGT390.2350388718134153No Hit
CCCTTCCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGCCGG370.22298559633580425No Hit
CTCCTTCCGGTGCTTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGCCGG340.20490568311938767No Hit
CTTCCTCTTTCAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCAAATTC330.19887904538058218No Hit
CTCCTTCCAGTGCTTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGCCGG320.19285240764177664No Hit
CTCCTTCCAGGTGCTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGCCGG310.18682576990297114No Hit
CTTCCTCTTCTCAGTGGGACAGGTGGGTAAACCTTTGGAGGGTCAAATTC310.18682576990297114No Hit
CTCCTTCCAGGTGCATCTGACCTGTTGTCGTACTTGTAGAACCATGTCGT300.1807991321641656No Hit
CTTCTCTTCTCAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCAAATTC290.17477249442536008No Hit
CTTCCTCTTCTCAGCTGAGACAGGTGGGTAAACCTTTGGAGGGTCAAATT280.1687458566865546No Hit
CTTCCTCTTCTCACTGGGACAGGTGGGTAAACCTTTGGAGGGTCAAATTC240.1446393057313325No Hit
CTTCCTCTTCTCGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCAAATTC240.1446393057313325No Hit
CTTCCTTTCTCAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCAAATTC230.13861266799252697No Hit
CTTCCTCTTCTCAGCAGTTGTTTTTCCGCGTACTTGTAGAACCATGTCGT220.13258603025372145No Hit
CTCCTTCCAGGTGCTTTTGCGTGGAGTGTGTGGGCCTCTTGGTGGGGCCG220.13258603025372145No Hit
CTCCTTCCAGGTGCTTTTGCGTGGAGTGTGCGGACCTCTTGGTGGGGCCG210.12655939251491594No Hit
CTTCCTCTCTCAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCAAATTC210.12655939251491594No Hit
CTTCCTCTTCCCAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCAAATT210.12655939251491594No Hit
CTCCTTCCAGGTGCTTTTGCGTGGAGTGTGTGGACCCCTTGGTGGGGCCG180.10847947929849937No Hit
CTTCCTCTTCTCAGCTGGACAGGTGGGTAAACCTTTGGAGGGTCAAATTC180.10847947929849937No Hit
CTCCTTCCAGGTGCTTTTGCGCGGAGTGTGTGGACCTCTTGGTGGGGCCG170.10245284155969384No Hit
CTTCCTCTTCTCAGCAGTTGTTGTTTCGCGTACTTGTAGAACCATGTCGT170.10245284155969384No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)