FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008144233

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008144233
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8960
Total Bases1.2 Mbp
Sequences flagged as poor quality0
Sequence length143-145
%GC63

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTTCCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGCCG552661.67410714285714No Hit
CTTCCTCTTCTCAGCAGTTGTTGTTTCCGCGTACTTGTAGAACCATGTCG99211.071428571428571No Hit
CTTCCTCTTCTCAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCAAATT5966.651785714285714No Hit
CTTCCTCTTCTCAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCCTAAG2662.96875No Hit
CTTCCTCTTCGCAGCAGTTGTTGTTTCCGCGTACTTGTAGAACCATGTCG1421.5848214285714284No Hit
CTTCCTCTTCTCAGCTGTTGTTGTTTCCGCGTACTTGTAGAACCATGTCG951.0602678571428572No Hit
CTCTTCCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGCCGG760.8482142857142857No Hit
CTCCTTCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGCCGG620.6919642857142857No Hit
CTCCTTCCAGGTGCTTTTGCGTGGAGTGTGGGGACCTCTTGGTGGGGCCG520.5803571428571429No Hit
CTCCTCCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGCCGG360.40178571428571425No Hit
CTCCTTCCAGGTGCATCTGACCTGTTGTCGTACTTGTAGAACCATGTCGT320.35714285714285715No Hit
TCCTTCCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGCCGG290.3236607142857143No Hit
CTCCTTCCAGTGCTTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGCCGG270.30133928571428575No Hit
CTCCTTCCGGTGCTTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGCCGG270.30133928571428575No Hit
CTCCTTCCAGGTGCTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGCCGG250.27901785714285715No Hit
CTTCCTCTTCTCAGCAGTTGTTGTTCCGCGTACTTGTAGAACCATGTCGT210.234375No Hit
CCCTTCCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGCCGG180.20089285714285712No Hit
CTCCTTCCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGCCC180.20089285714285712No Hit
CTCCTTCCAGGTGCTTTTGCGTGGAGTGTGTGGGCCTCTTGGTGGGGCCG180.20089285714285712No Hit
CTCCTTCCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTGGGTGGGGCCG150.16741071428571427No Hit
CTCCTTCCAGGTGCTTTTGCGTGGAGTGTGCGGACCTCTTGGTGGGGCCG130.14508928571428573No Hit
CTCCTTCCAGGTGCTTCTGACCTGTTGTCGTACTTGTAGAACCATGTCGT130.14508928571428573No Hit
CTCCTTCCAGGTGCTTTTGTGTGGAGTGTGTGGACCTCTTGGTGGGGCCG120.13392857142857142No Hit
CTCCTTCCAGGGCTTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGCCGG120.13392857142857142No Hit
CTCCTTCCAGGTGCTTTTGCGTGGAGTGTGTGGACCTATTGGTGGGGCCG120.13392857142857142No Hit
CTCCTTCCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGCCA120.13392857142857142No Hit
CTCCTTCCAGGTGCTTTTGCTCCAAGGAGTGCCTGCTCATTTCAAACAGG100.11160714285714285No Hit
CTTCCTCTTCTCAGCAGTTGTTTTTCCGCGTACTTGTAGAACCATGTCGT90.10044642857142856No Hit
CTCCTTCCAGGTGCTTTTGCGTGGAGTGTGTGGACCCCTTGGTGGGGCCG90.10044642857142856No Hit
CTTCCTTTCTCAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCAAATTC90.10044642857142856No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)