FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008144286

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008144286
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1773
Total Bases257 kbp
Sequences flagged as poor quality0
Sequence length142-145
%GC58

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAACAACAACTGCTGAGAAGAGGAAGCAAGGAAGACCAAGTCTCTGC111162.66215454032713No Hit
CGGAAACAACAACTGCTGCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCT22812.859560067681894No Hit
CGGAAACAACAACAGCTGAGAAGAGGAAGCAAGGAAGACCAAGTCTCTGC683.835307388606881No Hit
CGGAAACAACAACTGCTGCGAAGAGGAAGCAAGGAAGACCAAGTCTCTGC673.778905809362662No Hit
ACAACAGGTCAGATGCACCTGGAAGGAGCAAGGAAGACCAAGTCTCTGCT301.6920473773265652No Hit
CGGAACAACAACTGCTGAGAAGAGGAAGCAAGGAAGACCAAGTCTCTGCT191.071630005640158No Hit
ACAACAGGTCAGAAGCACCTGGAAGGAGCAAGGAAGACCAAGTCTCTGCT160.9024252679075015No Hit
CGGAAAAACAACTGCTGAGAAGAGGAAGCAAGGAAGACCAAGTCTCTGCT110.6204173716864072No Hit
ACAACAGGTCAGATGCAGGCGCTGCCCATGGGAGCCCTGCCCCAGGGGCC100.5640157924421884No Hit
CGGAAACACAACTGCTGAGAAGAGGAAGCAAGGAAGACCAAGTCTCTGCT80.4512126339537508No Hit
CGGAACAACAACTGCTGCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTT70.39481105470953193No Hit
CGGAAACAACACTGCTGAGAAGAGGAAGCAAGGAAGACCAAGTCTCTGCT50.2820078962210942No Hit
CGGAAACAACAACTGCTGAGAAGGGAAGCAAGGAAGACCAAGTCTCTGCT50.2820078962210942No Hit
GGAAACAACAACTGCTGAGAAGAGGAAGCAAGGAAGACCAAGTCTCTGCT50.2820078962210942No Hit
CGGAAACAACAACTGCTGAGAAGAGGAAGCAAGGAAGACCAAGACTCTGC40.2256063169768754No Hit
CGGAAACAACAACCGCTGAGAAGAGGAAGCAAGGAAGACCAAGTCTCTGC40.2256063169768754No Hit
CGAAACAACAACTGCTGAGAAGAGGAAGCAAGGAAGACCAAGTCTCTGCT40.2256063169768754No Hit
CGGAAACAACAACTGCTGAGAAAGGAAGCAAGGAAGACCAAGTCTCTGCT30.1692047377326565No Hit
CGGAAACAACAACTGTTGAGAAGAGGAAGCAAGGAAGACCAAGTCTCTGC30.1692047377326565No Hit
CGGAAACAACAACGCTGAGAAGAGGAAGCAAGGAAGACCAAGTCTCTGCT30.1692047377326565No Hit
CGGAAACAACAACTGCTGAGAAGAGGAACAAGGAAGACCAAGTCTCTGCT30.1692047377326565No Hit
ACAACAGGTCAGGATGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT30.1692047377326565Clontech SMARTer II A Oligonucleotide (96% over 25bp)
CGAAACAACAACTGCTGCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTT30.1692047377326565No Hit
CGGAAACAACAACTGCTGAGAAGAGAAGCAAGGAAGACCAAGTCTCTGCT30.1692047377326565No Hit
CGGAAACAACAACTGCTGAGAAGAGGAGCAAGGAAGACCAAGTCTCTGCT30.1692047377326565No Hit
CGGAAACAACAACTGCCGAGAAGAGGAAGCAAGGAAGACCAAGTCTCTGC30.1692047377326565No Hit
CGGAAACAAAACTGCTGAGAAGAGGAAGCAAGGAAGACCAAGTCTCTGCT20.1128031584884377No Hit
ACAACAGGTCAGATGCAGGGTACATGGGAAGCAGTGGTATCAACGCAGAG20.1128031584884377Clontech Universal Primer Mix Long (100% over 22bp)
CGGAAACAACAACTGCTGCGGGTGCTTTTGCGTGGAGTGTGTGGACCTCT20.1128031584884377No Hit
CGGAAACAACAACTGCTGCAGGTGCTTTTGCGTGGAGTGTGTGGGCCTCT20.1128031584884377No Hit
CGGAACAACAACTGCTGCGAAGAGGAAGCAAGGAAGACCAAGTCTCTGCT20.1128031584884377No Hit
CGAAACAACAACTGCTGCGAAGAGGAAGCAAGGAAGACCAAGTCTCTGCT20.1128031584884377No Hit
CGGAAACAACAACTACTGAGAAGAGGAAGCAAGGAAGACCAAGTCTCTGC20.1128031584884377No Hit
CGGAAACAACAACTGCTGGGAAGAGGAAGCAAGGAAGACCAAGTCTCTGC20.1128031584884377No Hit
CGGAAACAACAACTGCTGAGAAGAGGAAGCAAGGAATACCAAGTCTCTGC20.1128031584884377No Hit
ACAACAGGTCAATGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTA20.1128031584884377Clontech Universal Primer Mix Long (96% over 25bp)
CGGAAACAACAACTGCTGCCACTGACGACATGGTTCTACAAGTACGCGGA20.1128031584884377No Hit
CGGAAACAACAACTGCTGAGAAAAGGAAGCAAGGAAGACCAAGTCTCTGC20.1128031584884377No Hit
CGGAAACAACAACTCTGAGAAGAGGAAGCAAGGAAGACCAAGTCTCTGCT20.1128031584884377No Hit
CGGAACAACAACAGCTGAGAAGAGGAAGCAAGGAAGACCAAGTCTCTGCT20.1128031584884377No Hit
CGGAAACAACAACTGCTGAAAGAGGAAGCAAGGAAGACCAAGTCTCTGCT20.1128031584884377No Hit
CGGAAACAACAACTGCTGAGAAGAGAAAGCAAGGAAGACCAAGTCTCTGC20.1128031584884377No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)