FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008144287

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008144287
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1773
Total Bases257 kbp
Sequences flagged as poor quality0
Sequence length143-145
%GC62

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCCTCTTCTCAGCAGTTGTTGTTTCCGCGTACTTGTAGAACCATGTCG109961.985335589396506No Hit
CTTCCTCTTCTCAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCAAATT22212.521150592216582No Hit
CTTCCTCTTCGCAGCAGTTGTTGTTTCCGCGTACTTGTAGAACCATGTCG663.7225042301184432No Hit
CTTCCTCTTCTCAGCTGTTGTTGTTTCCGCGTACTTGTAGAACCATGTCG653.6661026508742243No Hit
CTCCTTCCAGGTGCATCTGACCTGTTGTCGTACTTGTAGAACCATGTCGT281.5792442188381277No Hit
CTTCCTCTTCTCAGCAGTTGTTGTTCCGCGTACTTGTAGAACCATGTCGT181.015228426395939No Hit
CTCCTTCCAGGTGCTTCTGACCTGTTGTCGTACTTGTAGAACCATGTCGT170.9588268471517203No Hit
CTTCCTCTTCTCAGCAGTTGTTTTTCCGCGTACTTGTAGAACCATGTCGT110.6204173716864072No Hit
CTCCTTCCAGGTGCTTTTGCTCCAAGGAGTGCCTGCTCATTTCAAACAGG110.6204173716864072No Hit
CTTCCTCTTCTCAGCAGTTGTGTTTCCGCGTACTTGTAGAACCATGTCGT80.4512126339537508No Hit
CTTCCTCTTCTCAGCAGTTGTTGTTTCCCGTACTTGTAGAACCATGTCGT50.2820078962210942No Hit
CTTCCCTTCTCAGCAGTTGTTGTTTCCGCGTACTTGTAGAACCATGTCGT50.2820078962210942No Hit
CTTCCTCTTCTCAGCAGTGTTGTTTCCGCGTACTTGTAGAACCATGTCGT50.2820078962210942No Hit
CTTCCTCTTCTAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCAAATTC50.2820078962210942No Hit
CTCCTTCCAGGTGCTTTGCGTTGATACCACTGCTTCCCATGTACTCTGCG50.2820078962210942Clontech Universal Primer Mix Long (95% over 22bp)
CTTCCTCTTCTCAGCAGTTGTTGTTTCGCGTACTTGTAGAACCATGTCGT40.2256063169768754No Hit
CTTCTCTTCTCAGCAGTTGTTGTTTCCGCGTACTTGTAGAACCATGTCGT40.2256063169768754No Hit
CTTCCTCTTCTCAGCGGTTGTTGTTTCCGCGTACTTGTAGAACCATGTCG40.2256063169768754No Hit
CTTCCTTTCTCAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCAAATTC40.2256063169768754No Hit
CTTCCTCTTCTCGGCAGTTGTTGTTTCCGCGTACTTGTAGAACCATGTCG30.1692047377326565No Hit
CTCCTCTTCTCAGCAGTTGTTGTTTCCGCGTACTTGTAGAACCATGTCGT30.1692047377326565No Hit
CTTCCTTTCTCAGCAGTTGTTGTTTCCGCGTACTTGTAGAACCATGTCGT30.1692047377326565No Hit
TTCCTCTTCTCAGCAGTTGTTGTTTCCGCGTACTTGTAGAACCATGTCGT30.1692047377326565No Hit
CTCCTTCCAGGTGCTTTTGCGTTGATACCACTGCTTCCCATGTACTCTGC30.1692047377326565Clontech Universal Primer Mix Long (95% over 21bp)
CTTCCTCTTCTCAGAGTTGTTGTTTCCGCGTACTTGTAGAACCATGTCGT30.1692047377326565No Hit
CTTCCTCTTCTCAACAGTTGTTGTTTCCGCGTACTTGTAGAACCATGTCG30.1692047377326565No Hit
CTTCCTCTTCTCAGCAGTTGTTGTTTCAGCGTACTTGTAGAACCATGTCG30.1692047377326565No Hit
CTTCCTCTTCTCAGCGTTGTTGTTTCCGCGTACTTGTAGAACCATGTCGT30.1692047377326565No Hit
CTTCCTCTTCTCAGCAGTTGTTGTTTCCGCGTACTTGTAGAACCATGTAG20.1128031584884377No Hit
CTTCCTCTTCTCAGCAGTTGTTGTTTCCGCGTACTTGTAGACCATGTCGT20.1128031584884377No Hit
CTTCCTCTTCTCAGCTGGACAGGTGGGTAAACCTTTGGAGGGTCAAATTC20.1128031584884377No Hit
CTTCCTCTTCTCGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCAAATTC20.1128031584884377No Hit
CTTCCTCTTCGCAGCAGTTGTTGTTTCGCGTACTTGTAGAACCATGTCGT20.1128031584884377No Hit
CTTCCTCTTCTCAGTAGTTGTTGTTTCCGCGTACTTGTAGAACCATGTCG20.1128031584884377No Hit
CTTCCTCTTCTCAGCTGGGACAGGTGGGTGAACCTTTGGAGGGTCAAATT20.1128031584884377No Hit
CTTCCTCTTCTCAGCTGTTGTTGTTCCGCGTACTTGTAGAACCATGTCGT20.1128031584884377No Hit
CTTTCTCTTCTCAGCAGTTGTTGTTTCCGCGTACTTGTAGAACCATGTCG20.1128031584884377No Hit
CTTCCTCTTCCCAGCAGTTGTTGTTTCCGCGTACTTGTAGAACCATGTCG20.1128031584884377No Hit
CTTCCTCTTCGCAGCAGTTGTTGTTCCGCGTACTTGTAGAACCATGTCGT20.1128031584884377No Hit
CTTCCTCTTCTCAGCAGTTTTGTTTCCGCGTACTTGTAGAACCATGTCGT20.1128031584884377No Hit
CTCCTTCCAGGTGCTTTTCGTTGATACCACTGCTTCCCATGTACTCTGCG20.1128031584884377No Hit
CTTCCTTTTCTCAGCAGTTGTTGTTTCCGCGTACTTGTAGAACCATGTCG20.1128031584884377No Hit
CTTCCTCTTCTCAGCAGTTGTTGTTTCCGCGTACTTGTAGAACCATGTTG20.1128031584884377No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)