FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008144293

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008144293
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1885
Total Bases273.3 kbp
Sequences flagged as poor quality0
Sequence length143-145
%GC62

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCCTCTTCTCAGCAGTTGTTGTTTCCGCGTACTTGTAGAACCATGTCG112759.78779840848807No Hit
CTTCCTCTTCTCAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCAAATT23012.20159151193634No Hit
CTTCCTCTTCTCAGCTGTTGTTGTTTCCGCGTACTTGTAGAACCATGTCG1045.517241379310345No Hit
CTTCCTCTTCGCAGCAGTTGTTGTTTCCGCGTACTTGTAGAACCATGTCG1005.305039787798409No Hit
CTCCTTCCAGGTGCATCTGACCTGTTGTCGTACTTGTAGAACCATGTCGT271.4323607427055705No Hit
CTCCTTCCAGGTGCTTCTGACCTGTTGTCGTACTTGTAGAACCATGTCGT211.1140583554376657No Hit
CTTCCTCTTCTCAGCAGTTGTTGTTCCGCGTACTTGTAGAACCATGTCGT191.0079575596816976No Hit
CTTCCTCTTCTCAGCAGTTGTTTTTCCGCGTACTTGTAGAACCATGTCGT150.7957559681697612No Hit
CTTCCTCTTCTCAGCAGTTGTTGTTTCGCGTACTTGTAGAACCATGTCGT80.42440318302387264No Hit
CTTCCTCTTCTCAGCAGTTGTGTTTCCGCGTACTTGTAGAACCATGTCGT70.3713527851458886No Hit
CTTCCTCTTCTCAGCAGTTGTTGGTTCCGCGTACTTGTAGAACCATGTCG50.2652519893899204No Hit
CTCCTTCCAGGTGCTTTTGCGTTGATACCACTGCTTCCCATGTACTCTGC50.2652519893899204Clontech Universal Primer Mix Long (95% over 21bp)
CTTCCTCTTCTCGGCAGTTGTTGTTTCCGCGTACTTGTAGAACCATGTCG40.21220159151193632No Hit
CTTCCTCTTCTCGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCAAATTC40.21220159151193632No Hit
CTTCCTCTTCTCAGCTGGGACAGGTGGGTAAACCTTTGGAGGGCCAAATT40.21220159151193632No Hit
CTTCCTCTTCTCAACAGTTGTTGTTTCCGCGTACTTGTAGAACCATGTCG40.21220159151193632No Hit
CTTCCTCTTCTCAGCGTTGTTGTTTCCGCGTACTTGTAGAACCATGTCGT40.21220159151193632No Hit
CTTCTCTTCTCAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCAAATTC30.15915119363395225No Hit
CTCCTCTTCTCAGCAGTTGTTGTTTCCGCGTACTTGTAGAACCATGTCGT30.15915119363395225No Hit
CTTCCTCTTCTCAGCAGTTGTTGTTTCCCGTACTTGTAGAACCATGTCGT30.15915119363395225No Hit
CTTCTCTTCTCAGCAGTTGTTGTTTCCGCGTACTTGTAGAACCATGTCGT30.15915119363395225No Hit
CTTCCTCTTCTCAGCGGTTGTTGTTTCCGCGTACTTGTAGAACCATGTCG30.15915119363395225No Hit
CTTCCTCTTCTCAGTAGTTGTTGTTTCCGCGTACTTGTAGAACCATGTCG30.15915119363395225No Hit
CTCCTTCCAGGTGCTTTTGCTCCAAGGAGTGCCTGCTCATTTCAAACAGG30.15915119363395225No Hit
CTTCCTCTTCTCAGCAGCAGTTGTTGTTTCCGCGTACTTGTAGAACCATG30.15915119363395225No Hit
CTCCTCTTCTCAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCAAATTC30.15915119363395225No Hit
CTTCCTCTCTCAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCAAATTC30.15915119363395225No Hit
CTTCCTCTTCTCAGCAGTTGTTGTTTCCGCGTACTTGTAGAACCATGTCT30.15915119363395225No Hit
CTTCCTCTTCTCAGCTGGGACAGGTGGGTAAACATTTGGAGGGTCAAATT20.10610079575596816No Hit
CTTCCTCTTCTCAGCAGTTGTTGTTTCCGCGTACTTGTAGAACCATGACG20.10610079575596816No Hit
CTCCTTCCAGGTGCCTCTGACCTGTTGTCGTACTTGTAGAACCATGTCGT20.10610079575596816No Hit
CTTCCTCTTCTCAGCTGGGACGGGTGGGTAAACCTTTGGAGGGTCAAATT20.10610079575596816No Hit
CTTCCTCTTTCAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCAAATTC20.10610079575596816No Hit
CTTCCTCTTCTCAGCTGTTGTTGTTTCGCGTACTTGTAGAACCATGTCGT20.10610079575596816No Hit
CTTCCTCTTCTCAGCAGTTGATGTTTCCGCGTACTTGTAGAACCATGTCG20.10610079575596816No Hit
CTTCCTCTTCTCAGCAGTGTTGTTCCGCGTACTTGTAGAACCATGTCGTC20.10610079575596816No Hit
CTTCCTCTTCTCAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCAAGTT20.10610079575596816No Hit
CTTCCTCTTCTCAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCTAATT20.10610079575596816No Hit
CTTCCTTTCGCAGCAGTTGTTGTTTCCGCGTACTTGTAGAACCATGTCGT20.10610079575596816No Hit
CTTCCTCTTCTCAGTGGGACAGGTGGGTAAACCTTTGGAGGGTCAAATTC20.10610079575596816No Hit
CTCCTTCCAGGTGCTTTTGCATCTGACCTGTTGTCGTACTTGTAGAACCA20.10610079575596816No Hit
CTTCCTCTTCTCAGCAGTTGTTGTTTCCGCGTACTTGTAGAACCAGGTCG20.10610079575596816No Hit
TTCCTCTTCTCAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCAAATTC20.10610079575596816No Hit
CTTCCTCTTCTCAGTTGTTGTTTCCGCGTACTTGTAGAACCATGTCGTCA20.10610079575596816No Hit
CTTCCTCCTCTCAGCAGTTGTTGTTTCCGCGTACTTGTAGAACCATGTCG20.10610079575596816No Hit
CTTCCTCTTCTCAGCAGTTGTTGTTTCCGCGTACTTGTAGAACCATGTCC20.10610079575596816No Hit
CTTCCTCTTCTCAGCAGTTGTTGTTTTCCGCGTACTTGTAGAACCATGTC20.10610079575596816No Hit
CTCCTTCCAGGTGCTTTTGACCTGTTGTCGTACTTGTAGAACCATGTCGT20.10610079575596816No Hit
CTTCCTCTTCTCAGCAGTTGTTGTTTCCACGTACTTGTAGAACCATGTCG20.10610079575596816No Hit
CTTCCTCTTCTCAGCAGTTTTGTTTCCGCGTACTTGTAGAACCATGTCGT20.10610079575596816No Hit
CTTCCTCTTCTCAGCAGTTGTTGTTTCAGCGTACTTGTAGAACCATGTCG20.10610079575596816No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)