FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008144306

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008144306
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8321
Total Bases1.2 Mbp
Sequences flagged as poor quality0
Sequence length142-145
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGC370444.513880543203946No Hit
CGGAAACAACAACTGCTGCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCT240528.902776108640786No Hit
CGGAAACAACAACTGCTGAGAAGAGGAAGCAAGGAAGACCAAGTCTCTGC102812.354284340824421No Hit
CGGAAACAACAACTGCTGCGAAGAGGAAGCAAGGAAGACCAAGTCTCTGC820.9854584785482514No Hit
CGGAAACAACAACAGCTGAGAAGAGGAAGCAAGGAAGACCAAGTCTCTGC630.7571205383968274No Hit
CGGAACAACAACTGCTGCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTT500.6008893161879583No Hit
CGGAACAACAACTGCTGAGAAGAGGAAGCAAGGAAGACCAAGTCTCTGCT260.3124624444177383No Hit
ACACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT260.3124624444177383No Hit
ACAACAGGTCAGATGCACCTGGAAGGAGCAAGGAAGACCAAGTCTCTGCT260.3124624444177383No Hit
ACAACAGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT220.2643912991227016No Hit
ACAAAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT210.25237351279894243No Hit
CGGAAAAACAACTGCTGCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTT180.21632015382766495No Hit
ACAACAGGTAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT180.21632015382766495No Hit
CGGAAACACAACTGCTGCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTT170.2043023675039058No Hit
CGAAACAACAACTGCTGCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTT170.2043023675039058No Hit
AAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT170.2043023675039058No Hit
CAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT160.19228458118014663No Hit
ACAACAGGTCAGAAGCACCTGGAAGGAGCAAGGAAGACCAAGTCTCTGCT150.18026679485638747No Hit
ACAACAGGCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT140.1682490085326283No Hit
GGAAACAACAACTGCTGCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTT120.14421343588510996No Hit
ACAACGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT120.14421343588510996No Hit
CGAAACAACAACTGCTGAGAAGAGGAAGCAAGGAAGACCAAGTCTCTGCT110.1321956495613508No Hit
ACAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGTGC110.1321956495613508No Hit
CGGAACCAACAACTGCTGCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCT110.1321956495613508No Hit
ACAACAGGTCAATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT110.1321956495613508No Hit
ACAACAGGTCAGATGCAGGCGCTGCCCATGGGAGCCCTGCCCCAGGGGCC110.1321956495613508No Hit
ACAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCCCCCACCCAGCGC90.10816007691383248No Hit
ACAACAGGTCAGTGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT90.10816007691383248No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)