FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008144310

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008144310
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8170
Total Bases1.1 Mbp
Sequences flagged as poor quality0
Sequence length143-145
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGC381146.64626682986536No Hit
CGGAAACAACAACTGCTGCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCT167520.501835985312116No Hit
CGGAAACAACAACTGCTGAGAAGAGGAAGCAAGGAAGACCAAGTCTCTGC135316.560587515299876No Hit
CGGAAACAACAACTGCTGCGAAGAGGAAGCAAGGAAGACCAAGTCTCTGC1471.799265605875153No Hit
CGGAAACAACAACAGCTGAGAAGAGGAAGCAAGGAAGACCAAGTCTCTGC1041.2729498164014688No Hit
ACAACAGGTCAGATGCACCTGGAAGGAGCAAGGAAGACCAAGTCTCTGCT400.4895960832313341No Hit
CGGAACAACAACTGCTGCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTT390.4773561811505508No Hit
CGGAACAACAACTGCTGAGAAGAGGAAGCAAGGAAGACCAAGTCTCTGCT260.3182374541003672No Hit
CGGAAACAACAACTGCTGCGGCGGCGAGAGGACTGGCCCTCCCGGCTCCA250.30599755201958384No Hit
ACACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT240.2937576499388005No Hit
AAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT210.25703794369645044No Hit
ACAAAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT210.25703794369645044No Hit
CGGAAAAACAACTGCTGAGAAGAGGAAGCAAGGAAGACCAAGTCTCTGCT150.18359853121175032No Hit
ACAACAGGTCAGAAGCACCTGGAAGGAGCAAGGAAGACCAAGTCTCTGCT150.18359853121175032No Hit
ACAACAGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT150.18359853121175032No Hit
CAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT150.18359853121175032No Hit
ACAACAGGCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT140.17135862913096694No Hit
ACAACAGGTAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT130.1591187270501836No Hit
ACAACGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT130.1591187270501836No Hit
ACAACAGGTCAGGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCG120.14687882496940025No Hit
ACAACAGGTCAGATGCAGGCCCATCGGTGCCCCAAGCTCCCACCCAGCGC120.14687882496940025No Hit
ACAACAGGTCAATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT120.14687882496940025No Hit
CGGAAACACAACTGCTGCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTT110.1346389228886169No Hit
CGGAAACACAACTGCTGAGAAGAGGAAGCAAGGAAGACCAAGTCTCTGCT110.1346389228886169No Hit
CGGAAACAACAACTGCTGCGGGTGCTTTTGCGTGGAGTGTGTGGACCTCT100.12239902080783352No Hit
ACAACAGGTCAGATGCAGGCCCATCGGCGTCCCAAGCTCCCACCCAGCGC100.12239902080783352No Hit
ACAACAGGTCAGATGAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT90.1101591187270502No Hit
CGAAACAACAACTGCTGCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTT90.1101591187270502No Hit
ACAACAGGTCAGATGCAGGCGCTGCCCATGGGAGCCCTGCCCCAGGGGCC90.1101591187270502No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)