FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008144387

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008144387
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9978
Total Bases1.4 Mbp
Sequences flagged as poor quality0
Sequence length136-145
%GC63

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTTCCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGCCG594359.56103427540589No Hit
CTTCCTCTTCTCAGCAGTTGTTGTTTCCGCGTACTTGTAGAACCATGTCG104910.513128883543796No Hit
CTTCCTCTTCTCAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCAAATT9099.110042092603727No Hit
CTTCCTCTTCTCAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCCTAAG3213.2170775706554418No Hit
CTCTTCCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGCCGG950.9520946081379035No Hit
CTTCCTCTTCTCAGCTGTTGTTGTTTCCGCGTACTTGTAGAACCATGTCG900.9019843656043296No Hit
CTTCCTCTTCGCAGCAGTTGTTGTTTCCGCGTACTTGTAGAACCATGTCG830.8318300260573261No Hit
CTCCTTCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGCCGG640.6414111044297455No Hit
TCCTTCCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGCCGG530.531168570855883No Hit
CTCCTCCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGCCGG430.4309480857887352No Hit
CTCCTTCCAGGTGCTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGCCGG410.4109039887753057No Hit
CTTCCTCTTCTCAGCAGTTGTTGTTCCGCGTACTTGTAGAACCATGTCGT330.3307276007215875No Hit
CTCCTTCCAGTGCTTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGCCGG310.31068350370815795No Hit
CTCCTTCCGGTGCTTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGCCGG260.2605732611745841No Hit
CCCTTCCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGCCGG240.24052916416115455No Hit
CTCCTTCCAGGTGCTTCTGACCTGTTGTCGTACTTGTAGAACCATGTCGT210.21046301864101025No Hit
CTCCTTCCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGCCC210.21046301864101025No Hit
CTCCTTCCAGGTGCATCTGACCTGTTGTCGTACTTGTAGAACCATGTCGT200.20044097013429546No Hit
CTCCTTCCAGGTGCTTTTGCGTGGAGTGTGTGGGCCTCTTGGTGGGGCCG190.19041892162758067No Hit
CTCCTTCCAGGTGCTTTTGCTCCAAGGAGTGCCTGCTCATTTCAAACAGG170.17037482461415115No Hit
CTCCTTCCAGGTGCTTTTGCGTGGAGTGTGCGGACCTCTTGGTGGGGCCG160.16035277610743637No Hit
CTCCTTCCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGTCG140.14030867909400682No Hit
CTCCTTCCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTGGGTGGGGCCG130.13028663058729206No Hit
CTTCCTCTTCTAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCAAATTC120.12026458208057728No Hit
CTCCTTCCAGGTGCTTTTGCGTGGAGTGCGTGGACCTCTTGGTGGGGCCG120.12026458208057728No Hit
CTCCTTCCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTTGGCGGGGCCG120.12026458208057728No Hit
CTCCTTCCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGACCG110.1102425335738625No Hit
CTCCTTCCAGGTGCTTTTGCGTGGAGTGTGTGGACCCCTTGGTGGGGCCG110.1102425335738625No Hit
CTTCCTCTTCTCAGCAGTTGTTTTTCCGCGTACTTGTAGAACCATGTCGT100.10022048506714773No Hit
CTTCCTCTTCTCAGCAGTTGTTGTTTCGCGTACTTGTAGAACCATGTCGT100.10022048506714773No Hit
CTCCTTCCAGGTGCTTTTGCGTGGAGCGTGTGGACCTCTTGGTGGGGCCG100.10022048506714773No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)