FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008144404

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008144404
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2786
Total Bases403.8 kbp
Sequences flagged as poor quality0
Sequence length143-145
%GC62

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAACAACAACTGCTGAGAAGAGGAAGCAAGGAAGACCAAGTCTCTGC98935.4989231873654No Hit
ACAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGC68424.55132806891601No Hit
CGGAAACAACAACTGCTGCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCT40914.680545585068197No Hit
CGGAAACAACAACTGCTGCGAAGAGGAAGCAAGGAAGACCAAGTCTCTGC1134.0559942569992815No Hit
CGGAAACAACAACAGCTGAGAAGAGGAAGCAAGGAAGACCAAGTCTCTGC772.763819095477387No Hit
ACAACAGGTCAGATGCAGGCCATGGAAACTGCTTGTAATATGTGTGATCC371.3280689160086145No Hit
ACAACAGGTCAGATGCACCTGGAAGGAGCAAGGAAGACCAAGTCTCTGCT250.8973438621679828No Hit
CGGAACAACAACTGCTGAGAAGAGGAAGCAAGGAAGACCAAGTCTCTGCT180.6460875807609476No Hit
ACAACAGGTCAGAAGCACCTGGAAGGAGCAAGGAAGACCAAGTCTCTGCT180.6460875807609476No Hit
CGGAAACACAACTGCTGAGAAGAGGAAGCAAGGAAGACCAAGTCTCTGCT110.3948312993539124No Hit
ACAACAGGTCAGATGCAGGCGCTGCCCATGGGAGCCCTGCCCCAGGGGCC70.25125628140703515No Hit
CGGAAACAACACTGCTGAGAAGAGGAAGCAAGGAAGACCAAGTCTCTGCT70.25125628140703515No Hit
CGGAAACACAACTGCTGCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTT70.25125628140703515No Hit
ACAACAGGTCAGATGCAGGCCAGTAGTGCAAGTTGAGCACAGCCTGCCAG60.21536252692031585No Hit
AAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT60.21536252692031585No Hit
CGAAACAACAACTGCTGAGAAGAGGAAGCAAGGAAGACCAAGTCTCTGCT60.21536252692031585No Hit
CGGAAACAACAACTGCTGAGAAGAGAAGCAAGGAAGACCAAGTCTCTGCT60.21536252692031585No Hit
ACAAAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT60.21536252692031585No Hit
ACACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT60.21536252692031585No Hit
CGGAACAACAACTGCTGCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTT60.21536252692031585No Hit
CGGAAACAACAACGCTGAGAAGAGGAAGCAAGGAAGACCAAGTCTCTGCT50.17946877243359655No Hit
ACAACAGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT50.17946877243359655No Hit
CGGAAACAACAACCGCTGAGAAGAGGAAGCAAGGAAGACCAAGTCTCTGC50.17946877243359655No Hit
CGGAAAAACAACTGCTGAGAAGAGGAAGCAAGGAAGACCAAGTCTCTGCT50.17946877243359655No Hit
CGGAAAAACAACTGCTGCGAAGAGGAAGCAAGGAAGACCAAGTCTCTGCT50.17946877243359655No Hit
CGGAAACAACAACTGCTGAGAAGAGGAAGCAAGGAAGACCAAGACTCTGC50.17946877243359655No Hit
ACAACAGGTCAAAAGCACCTGGAAGGAGCAAGGAAGACCAAGTCTCTGCT50.17946877243359655No Hit
CGGAACCAACAACTGCTGAGAAGAGGAAGCAAGGAAGACCAAGTCTCTGC50.17946877243359655No Hit
GGAAACAACAACTGCTGAGAAGAGGAAGCAAGGAAGACCAAGTCTCTGCT50.17946877243359655No Hit
CGGAAAAACAACTGCTGCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTT40.14357501794687724No Hit
CGGAAACAACAACTGCTGAGAAGAGGAAGCAAGGAAGACCAAGTCTCAGC40.14357501794687724No Hit
ACAACAGGTCAGGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCG40.14357501794687724No Hit
ACAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCCCCCACCCAGCGC40.14357501794687724No Hit
ACAACAGGTCAGATGCAGGCCCATCGGTGCCCCAAGCTCCCACCCAGCGC40.14357501794687724No Hit
CGGAACCAACAACTGCTGCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCT30.10768126346015792No Hit
ACAACAGGTCAATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT30.10768126346015792No Hit
CGGAAACAACAACTGCTGGGAAGAGGAAGCAAGGAAGACCAAGTCTCTGC30.10768126346015792No Hit
ACAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAACTCCCACCCAGCGC30.10768126346015792No Hit
ACAACAGGTCAGATGCACCTGAAGGAGCAAGGAAGACCAAGTCTCTGCTA30.10768126346015792No Hit
CGGAAACAACAGCTGAGAAGAGGAAGCAAGGAAGACCAAGTCTCTGCTAC30.10768126346015792No Hit
ACAACAGGCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT30.10768126346015792No Hit
CGGAAACAACAACTGCTGAGAAGAGGAACAAGGAAGACCAAGTCTCTGCT30.10768126346015792No Hit
CGGAAACAACAACTGAGAAGAGGAAGCAAGGAAGACCAAGTCTCTGCTAC30.10768126346015792No Hit
CGGAAACAACACTGCTGCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTT30.10768126346015792No Hit
ACAACAGGTCAATGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTA30.10768126346015792Clontech Universal Primer Mix Long (96% over 25bp)
CGAAACAACAACTGCTGCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTT30.10768126346015792No Hit
CGGAAACAACAACTGCTGAGAAGAGGAGCAAGGAAGACCAAGTCTCTGCT30.10768126346015792No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)