FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008144446

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008144446
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8998
Total Bases1.3 Mbp
Sequences flagged as poor quality0
Sequence length143-145
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGC611968.00400088908647No Hit
CGGAAACAACAACTGCTGAGAAGAGGAAGCAAGGAAGACCAAGTCTCTGC124513.836408090686819No Hit
CGGAAACAACAACTGCTGCGGCGGCGAGAGGACTGGCCCTCCCGGCTCCA1661.8448544120915762No Hit
CGGAAACAACAACTGCTGCGAAGAGGAAGCAAGGAAGACCAAGTCTCTGC971.0780173371860413No Hit
CGGAAACAACAACAGCTGAGAAGAGGAAGCAAGGAAGACCAAGTCTCTGC810.9002000444543232No Hit
ACACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT640.7112691709268726No Hit
ACAACAGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT380.4223160702378307No Hit
ACAAAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT370.41120248944209825No Hit
CAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT310.3445210046677039No Hit
AAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT300.3334074238719716No Hit
ACAACAGGTAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT270.3000666814847744No Hit
ACAACAGGTCAATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT270.3000666814847744No Hit
ACAACAGGTCGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT250.2778395198933096No Hit
ACAACAGGTCAGAAGCACCTGGAAGGAGCAAGGAAGACCAAGTCTCTGCT240.26672593909757725No Hit
ACAACAGGTCAGATGCACCTGGAAGGAGCAAGGAAGACCAAGTCTCTGCT240.26672593909757725No Hit
ACAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAACTCCCACCCAGCGC230.25561235830184487No Hit
CGGAACAACAACTGCTGAGAAGAGGAAGCAAGGAAGACCAAGTCTCTGCT210.23338519671038008No Hit
ACAACAGGCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT210.23338519671038008No Hit
ACAACAGGTCAGATGCAGGCCCATCGGCGTCCCAAGCTCCCACCCAGCGC210.23338519671038008No Hit
ACAACAGGTCAGATGCAGGCCCATCGATGTCCCAAGCTCCCACCCAGCGC190.21115803511891534No Hit
ACAACAGGTCAGATGCAGGCCCATCGGTGCCCCAAGCTCCCACCCAGCGC190.21115803511891534No Hit
ACAACGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT190.21115803511891534No Hit
ACAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCCCCCACCCAGCGC160.17781729273171815No Hit
ACAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCAC150.1667037119359858No Hit
ACAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGTGC150.1667037119359858No Hit
ACAACAGGTCAGTGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT150.1667037119359858No Hit
ACAACAGGTCAGATCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT150.1667037119359858No Hit
CGGAAAAACAACTGCTGAGAAGAGGAAGCAAGGAAGACCAAGTCTCTGCT140.15559013114025338No Hit
CGAAACAACAACTGCTGAGAAGAGGAAGCAAGGAAGACCAAGTCTCTGCT120.13336296954878862No Hit
ACAACAGGTCAGATGTAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGC120.13336296954878862No Hit
ACAACAGGTCAGATGCAGGTCCATCGGTGTCCCAAGCTCCCACCCAGCGC120.13336296954878862No Hit
CGGAAACAACAACTGCTGAGAAGAGGAAGCAAGGAAGACCAAGACTCTGC110.12224938875305623No Hit
ACAACAGGTCAGGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCG110.12224938875305623No Hit
ACAACAGGTCAGATGCAGGCCCATTGGTGTCCCAAGCTCCCACCCAGCGC100.11113580795732383No Hit
ACAACAGGTCAGATGCGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT100.11113580795732383No Hit
ACAACAGGTCAGATGCAGGCCCGTCGGTGTCCCAAGCTCCCACCCAGCGC90.10002222716159147No Hit
ACAACAGGTCAGATGCAGGCCCACCGGTGTCCCAAGCTCCCACCCAGCGC90.10002222716159147No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)