FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008144545

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008144545
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2299
Total Bases333.3 kbp
Sequences flagged as poor quality0
Sequence length143-145
%GC62

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCCTCTTCTCAGCAGTTGTTGTTTCCGCGTACTTGTAGAACCATGTCG151065.68073075250108No Hit
CTTCCTCTTCTCAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCAAATT1155.002174858634189No Hit
CTTCCTCTTCGCAGCAGTTGTTGTTTCCGCGTACTTGTAGAACCATGTCG833.6102653327533707No Hit
CTTCCTCTTCTCAGCTGTTGTTGTTTCCGCGTACTTGTAGAACCATGTCG803.4797738147020447No Hit
CTCCTTCCAGGTGCATCTGACCTGTTGTCGTACTTGTAGAACCATGTCGT431.8703784254023488No Hit
CTTCCTCTTCTCAGCAGTTGTTGTTCCGCGTACTTGTAGAACCATGTCGT391.6963897346672467No Hit
CTCCTTCCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGCCG361.5658982166159199No Hit
CTCCTTCCAGGTGCTTCTGACCTGTTGTCGTACTTGTAGAACCATGTCGT301.3049151805132666No Hit
CTTCCTCTTCTCAGCAGTTGTTTTTCCGCGTACTTGTAGAACCATGTCGT241.0439321444106133No Hit
CTCCTTCCAGGTGCTTTTGCTCCAAGGAGTGCCTGCTCATTTCAAACAGG160.6959547629404089No Hit
CTTCCTCTTCTCAGCAGTTGTTGTTTCGCGTACTTGTAGAACCATGTCGT140.6089604175728578No Hit
CTTCTCTTCTCAGCAGTTGTTGTTTCCGCGTACTTGTAGAACCATGTCGT110.4784688995215311No Hit
CTTCCTCTTCTCAGCAGTTGTGTTTCCGCGTACTTGTAGAACCATGTCGT110.4784688995215311No Hit
CTTCCTCTTCTCAGCAGTGTTGTTTCCGCGTACTTGTAGAACCATGTCGT90.39147455415397997No Hit
CTTCCTCTTCTCAGCGTTGTTGTTTCCGCGTACTTGTAGAACCATGTCGT70.3044802087864289No Hit
CTCCTTCCAGGTGCTTTTGCAGACAGACCACCACTGAGGTGCACTTGCCC60.26098303610265333No Hit
CTTCCTCTTCTCAGTAGTTGTTGTTTCCGCGTACTTGTAGAACCATGTCG60.26098303610265333No Hit
CTTCCTCTTCTCAGCAGTTGTTGGTTCCGCGTACTTGTAGAACCATGTCG60.26098303610265333No Hit
CTTCCTTTCTCAGCAGTTGTTGTTTCCGCGTACTTGTAGAACCATGTCGT60.26098303610265333No Hit
CTTCCTCTCTCAGCAGTTGTTGTTTCCGCGTACTTGTAGAACCATGTCGT50.2174858634188778No Hit
CTTCCTCTTCTCAACAGTTGTTGTTTCCGCGTACTTGTAGAACCATGTCG50.2174858634188778No Hit
CTCCTCTTCTCAGCAGTTGTTGTTTCCGCGTACTTGTAGAACCATGTCGT40.17398869073510223No Hit
TTCCTCTTCTCAGCAGTTGTTGTTTCCGCGTACTTGTAGAACCATGTCGT40.17398869073510223No Hit
CTTCCTCTTCTCAGAGTTGTTGTTTCCGCGTACTTGTAGAACCATGTCGT40.17398869073510223No Hit
CTTCCTCTTTCAGCAGTTGTTGTTTCCGCGTACTTGTAGAACCATGTCGT40.17398869073510223No Hit
CTTCCTCTTCTCAGCAGTTTTGTTTCCGCGTACTTGTAGAACCATGTCGT40.17398869073510223No Hit
CTTCCTCTTCTCGGCAGTTGTTGTTTCCGCGTACTTGTAGAACCATGTCG30.13049151805132667No Hit
CTTCCTCTTCTCAGCGGTTGTTGTTTCCGCGTACTTGTAGAACCATGTCG30.13049151805132667No Hit
CTTCTCTTCTCAGCTGTTGTTGTTTCCGCGTACTTGTAGAACCATGTCGT30.13049151805132667No Hit
CTTCCCCTTCTCAGCAGTTGTTGTTTCCGCGTACTTGTAGAACCATGTCG30.13049151805132667No Hit
CTCCTTCCAGGTGCTTTTGACCTGTTGTCGTACTTGTAGAACCATGTCGT30.13049151805132667No Hit
CTTCCTCTTCTCAGCAGTTGTTGTTTCCGCGTACTTGTAGACCATGTCGT30.13049151805132667No Hit
CTTCCTCTTCTCAGCAGTTGTTGTTTCCCGTACTTGTAGAACCATGTCGT30.13049151805132667No Hit
ATTCCTCTTCTCAGCAGTTGTTGTTTCCGCGTACTTGTAGAACCATGTCG30.13049151805132667No Hit
CTTCCTCTTCCCAGCAGTTGTTGTTTCCGCGTACTTGTAGAACCATGTCG30.13049151805132667No Hit
CTTCCTCTTCTCAGCAGCAGTTGTTGTTTCCGCGTACTTGTAGAACCATG30.13049151805132667No Hit
CTTCCTCCTCTCAGCAGTTGTTGTTTCCGCGTACTTGTAGAACCATGTCG30.13049151805132667No Hit
CTTCCTCTTCTCAGCAGTTGTTGTTTCCGCGTACTTGTAGATCCATGTCG30.13049151805132667No Hit
CTTCCTCTTCGCAGCAGTTGTTGTTCCGCGTACTTGTAGAACCATGTCGT30.13049151805132667No Hit
CTCCTTCCAGGTGCTTTGCGTTGATACCACTGCTTCCCATGTACTCTGCG30.13049151805132667Clontech Universal Primer Mix Long (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)