FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008144576

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008144576
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5888
Total Bases853.2 kbp
Sequences flagged as poor quality0
Sequence length142-145
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGC370662.941576086956516No Hit
CGGAAACAACAACTGCTGAGAAGAGGAAGCAAGGAAGACCAAGTCTCTGC116219.735054347826086No Hit
CGGAAACAACAACAGCTGAGAAGAGGAAGCAAGGAAGACCAAGTCTCTGC540.9171195652173914No Hit
ACAACAGGTCAGATGCACCTGGAAGGAGCAAGGAAGACCAAGTCTCTGCT530.9001358695652174No Hit
ACAACAGGTCAGAAGCACCTGGAAGGAGCAAGGAAGACCAAGTCTCTGCT490.8322010869565218No Hit
CGGAAACAACAACTGCTGCGGCGGCGAGAGGACTGGCCCTCCCGGCTCCA420.7133152173913043No Hit
CGGAAACAACAACTGCTGCGAAGAGGAAGCAAGGAAGACCAAGTCTCTGC420.7133152173913043No Hit
ACACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT370.6283967391304348No Hit
CGGAACAACAACTGCTGAGAAGAGGAAGCAAGGAAGACCAAGTCTCTGCT300.5095108695652174No Hit
ACAAAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT270.4585597826086957No Hit
AAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT240.4076086956521739No Hit
CAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT170.2887228260869565No Hit
ACAACAGGCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT170.2887228260869565No Hit
CGGAAAAACAACTGCTGAGAAGAGGAAGCAAGGAAGACCAAGTCTCTGCT160.2717391304347826No Hit
ACAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCCCCCACCCAGCGC160.2717391304347826No Hit
ACAACAGGTAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT150.2547554347826087No Hit
ACAACAGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT140.2377717391304348No Hit
ACAACAGGTCAATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT130.22078804347826086No Hit
ACAACAGGTCAGATGCAGGTCCATCGGTGTCCCAAGCTCCCACCCAGCGC130.22078804347826086No Hit
ACAACGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT120.20380434782608695No Hit
ACAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAACTCCCACCCAGCGC100.16983695652173914No Hit
ACAACAGGTCAGATGCGGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGC100.16983695652173914No Hit
ACAACAGGTCAGATGCAGGCCCATCGGTGCCCCAAGCTCCCACCCAGCGC100.16983695652173914No Hit
CGGAAACACAACTGCTGAGAAGAGGAAGCAAGGAAGACCAAGTCTCTGCT90.15285326086956522No Hit
ACAACAGGTCAGATGCAGGCCCATCGGCGTCCCAAGCTCCCACCCAGCGC90.15285326086956522No Hit
CGGAAACAACAACTGCTGAGAAGAGAAGCAAGGAAGACCAAGTCTCTGCT90.15285326086956522No Hit
ACAACAGGTCAGAGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT90.15285326086956522No Hit
ACAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGTGC90.15285326086956522No Hit
ACAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCAC80.1358695652173913No Hit
CGAAACAACAACTGCTGAGAAGAGGAAGCAAGGAAGACCAAGTCTCTGCT80.1358695652173913No Hit
ACAACAGGTCAGTGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT80.1358695652173913No Hit
CGGAAACAACAACTGCTGAGAAAGGAAGCAAGGAAGACCAAGTCTCTGCT70.1188858695652174No Hit
ACAACAGGTCAGATGCAGGCCCATCGATGTCCCAAGCTCCCACCCAGCGC70.1188858695652174No Hit
CGGAAACAACAACTGTTGAGAAGAGGAAGCAAGGAAGACCAAGTCTCTGC70.1188858695652174No Hit
ACAACAGGTCAGATGCAGGCCCACCGGTGTCCCAAGCTCCCACCCAGCGC70.1188858695652174No Hit
ACAACAGGTCAGGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCG70.1188858695652174No Hit
ACAACAGGTCAGATGCAGGCCCATCGGTGTCCCGAGCTCCCACCCAGCGC70.1188858695652174No Hit
CGGAAACAACAACTGCTGAGAAGAGGAGCAAGGAAGACCAAGTCTCTGCT70.1188858695652174No Hit
GGAAACAACAACTGCTGAGAAGAGGAAGCAAGGAAGACCAAGTCTCTGCT70.1188858695652174No Hit
CGGAAACAAAACTGCTGAGAAGAGGAAGCAAGGAAGACCAAGTCTCTGCT60.10190217391304347No Hit
ACAACAGGTCAGATGCAGGCCCATCAGTGTCCCAAGCTCCCACCCAGCGC60.10190217391304347No Hit
ACAACAGGTCAGATGCAGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT60.10190217391304347No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)