FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008144582

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008144582
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2104
Total Bases305 kbp
Sequences flagged as poor quality0
Sequence length142-145
%GC60

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAACAACAACTGCTGCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCT87341.49239543726236No Hit
CGGAAACAACAACTGCTGAGAAGAGGAAGCAAGGAAGACCAAGTCTCTGC71133.79277566539924No Hit
CGGAAACAACAACTGCTGCGAAGAGGAAGCAAGGAAGACCAAGTCTCTGC1135.3707224334600765No Hit
CGGAAACAACAACAGCTGAGAAGAGGAAGCAAGGAAGACCAAGTCTCTGC703.326996197718631No Hit
ACAACAGGTCAGATGCACCTGGAAGGAGCAAGGAAGACCAAGTCTCTGCT200.9505703422053232No Hit
CGGAACAACAACTGCTGAGAAGAGGAAGCAAGGAAGACCAAGTCTCTGCT190.9030418250950569No Hit
CGGAACAACAACTGCTGCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTT160.7604562737642585No Hit
CGGAAAAACAACTGCTGCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTT120.5703422053231939No Hit
ACAACAGGTCAGAAGCACCTGGAAGGAGCAAGGAAGACCAAGTCTCTGCT90.42775665399239543No Hit
ACAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGC80.38022813688212925No Hit
CGGAAACACAACTGCTGAGAAGAGGAAGCAAGGAAGACCAAGTCTCTGCT70.3326996197718631No Hit
CGGAAACACAACTGCTGCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTT70.3326996197718631No Hit
CGGAAACAACAACTGCTGAGAAGAGGAAGCAAGGAAGACCAAGTCTCAGC50.2376425855513308No Hit
CGGAAACAACACTGCTGCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTT40.19011406844106463No Hit
ACAACAGGTCAATGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTA40.19011406844106463Clontech Universal Primer Mix Long (96% over 25bp)
CGGAAACAACAACTGCTGCAGGTGCCTTTGCGTGGAGTGTGTGGACCTCT40.19011406844106463No Hit
CGAAACAACAACTGCTGAGAAGAGGAAGCAAGGAAGACCAAGTCTCTGCT40.19011406844106463No Hit
CGGAAACAACAACTGTTGCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCT40.19011406844106463No Hit
GGAAACAACAACTGCTGAGAAGAGGAAGCAAGGAAGACCAAGTCTCTGCT40.19011406844106463No Hit
CGGAAACAACAACTGCGCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTT30.14258555133079848No Hit
CGGAAACAACACTGCTGAGAAGAGGAAGCAAGGAAGACCAAGTCTCTGCT30.14258555133079848No Hit
CGGAAACAACAACTGCTGCAGGTGCTTTTGTGTGGAGTGTGTGGACCTCT30.14258555133079848No Hit
CGGAAACAACAACTACTGAGAAGAGGAAGCAAGGAAGACCAAGTCTCTGC30.14258555133079848No Hit
CGGAAACAACAACTGCTGGGAAGAGGAAGCAAGGAAGACCAAGTCTCTGC30.14258555133079848No Hit
CGGAAACAACAACTGCTGCTGAGAAGAGGAAGCAAGGAAGACCAAGTCTC30.14258555133079848No Hit
CGGAAAAACAACTGCTGAGAAGAGGAAGCAAGGAAGACCAAGTCTCTGCT30.14258555133079848No Hit
CGGAAACAACAACTGCTGCAGGTGCTTTTGCGTGGAGTGTGTGGACCCCT30.14258555133079848No Hit
CGGAAACAAAACTGCTGCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTT30.14258555133079848No Hit
CGAAACAACAACTGCTGCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTT30.14258555133079848No Hit
CGGAAACAACAACTGCTGCAGGTGCTTTTGCGTGGAGTGTGCGGACCTCT30.14258555133079848No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)