FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008144589

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008144589
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8181
Total Bases1.1 Mbp
Sequences flagged as poor quality0
Sequence length136-145
%GC63

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTTCCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGCCG492860.237134824593575No Hit
CTTCCTCTTCTCAGCAGTTGTTGTTTCCGCGTACTTGTAGAACCATGTCG106413.005745018946339No Hit
CTTCCTCTTCTCAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCAAATT5386.576213176873242No Hit
CTTCCTCTTCGCAGCAGTTGTTGTTTCCGCGTACTTGTAGAACCATGTCG1541.8824104632685492No Hit
CTTCCTCTTCTCAGCTGTTGTTGTTTCCGCGTACTTGTAGAACCATGTCG1281.564600904534898No Hit
CTCTTCCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGCCGG901.1001100110011002No Hit
CTTCCTCTTCTCAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCCTAAG760.9289817870675957No Hit
CTCCTTCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGCCGG490.5989487837672656No Hit
CTCCTCCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGCCGG410.5011612272338345No Hit
CTCCTTCCAGGTGCTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGCCGG360.44004400440044No Hit
TCCTTCCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGCCGG310.37892678156704557No Hit
CCCTTCCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGCCGG240.29336266960029334No Hit
CTTCCTCTTCTCAGCAGTTGTTGTTCCGCGTACTTGTAGAACCATGTCGT230.2811392250336145No Hit
CTCCTTCCAGGTGCATCTGACCTGTTGTCGTACTTGTAGAACCATGTCGT220.26891578046693554No Hit
CTCCTTCCAGTGCTTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGCCGG200.2444688913335778No Hit
CTCCTTCCAGGTGCTTCTGACCTGTTGTCGTACTTGTAGAACCATGTCGT170.20779855763354113No Hit
CTCCTTCCAGGTGCTTTTGCGTGGAGTGTGCGGACCTCTTGGTGGGGCCG160.19557511306686226No Hit
CTCCTTCCAGGTGCTTTTGCGTGGAGTGTGTGGGCCTCTTGGTGGGGCCG160.19557511306686226No Hit
CTCCTTCCGGTGCTTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGCCGG150.18335166850018336No Hit
CTCCTTCCAGGTGCTTTTGCATGGAGTGTGTGGACCTCTTGGTGGGGCCG130.15890477936682557No Hit
CCCCTTCCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGCCG120.14668133480014667No Hit
CTCCTTCCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTGGGTGGGGCCG120.14668133480014667No Hit
CTTCCTCTTCTCAGCAGTTGTTTTTCCGCGTACTTGTAGAACCATGTCGT110.13445789023346777No Hit
CTCCTTCCAGGTGCTTTTCGTGGAGTGTGTGGACCTCTTGGTGGGGCCGG110.13445789023346777No Hit
CTCCTTCCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCCTGGTGGGGCCG110.13445789023346777No Hit
CTCCTTCCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTTGGCGGGGCCG110.13445789023346777No Hit
CTCCTTCCAGGTGCTTTTGCGCGGAGTGTGTGGACCTCTTGGTGGGGCCG100.1222344456667889No Hit
CTCCTTCCAGGTGCTTTTGTGTGGAGTGTGTGGACCTCTTGGTGGGGCCG100.1222344456667889No Hit
CTCCTTCCAGGTGCTTTTGCGTGGGGTGTGTGGACCTCTTGGTGGGGCCG100.1222344456667889No Hit
CTTCCTCTTCTCAGCAGTTGTGTTTCCGCGTACTTGTAGAACCATGTCGT100.1222344456667889No Hit
CTCCTTCCAGGTGCTTTTGCGTGGAGTGTGTGGACCCCTTGGTGGGGCCG90.11001100110011No Hit
CTCCTTCCAGGGCTTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGCCGG90.11001100110011No Hit
CTCCTTCCAGGTGATTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGCCG90.11001100110011No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)