FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008144590

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008144590
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22880
Total Bases3.3 Mbp
Sequences flagged as poor quality0
Sequence length142-145
%GC63

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAACAACAACTGCTGCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCT1197052.31643356643356No Hit
ACAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGC579325.319055944055947No Hit
CGGAAACAACAACTGCTGAGAAGAGGAAGCAAGGAAGACCAAGTCTCTGC16797.338286713286714No Hit
CGGAACAACAACTGCTGCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTT2361.0314685314685315No Hit
CGGAAACAACAACTGCTGCGAAGAGGAAGCAAGGAAGACCAAGTCTCTGC2050.895979020979021No Hit
CGGAAACAACAACAGCTGAGAAGAGGAAGCAAGGAAGACCAAGTCTCTGC1550.6774475524475524No Hit
CGGAAAAACAACTGCTGCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTT850.3715034965034965No Hit
CGAAACAACAACTGCTGCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTT820.3583916083916084No Hit
CGGAAACACAACTGCTGCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTT790.34527972027972026No Hit
CGGAAACAACAACTGCTGCAGGTGCCTTTGCGTGGAGTGTGTGGACCTCT510.2229020979020979No Hit
ACAAAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT500.21853146853146854No Hit
ACACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT450.1966783216783217No Hit
CGGAAACAACAACTGCTGCGGGTGCTTTTGCGTGGAGTGTGTGGACCTCT430.18793706293706294No Hit
GGAAACAACAACTGCTGCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTT420.18356643356643357No Hit
CGGAAACAACACTGCTGCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTT390.17045454545454544No Hit
CGGAAACAACAACTGCTGCAGGTGCTTTTGCGTGGAGTGCGTGGACCTCT340.1486013986013986No Hit
CGGAAACAACAACTGCTGCAGGTGCTTTTGCGTGGAGTGTGTGGGCCTCT310.13548951048951047No Hit
AAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT310.13548951048951047No Hit
ACAACAGGTAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT300.13111888111888112No Hit
ACAACAGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT300.13111888111888112No Hit
CAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT300.13111888111888112No Hit
CGGAAACAACAACTGCTGCAGGTGCTTTTGCGTGGGGTGTGTGGACCTCT290.12674825174825174No Hit
CGGAAACAAAACTGCTGCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTT290.12674825174825174No Hit
CGGAAACAACAACTGCTGCAGGCGCTTTTGCGTGGAGTGTGTGGACCTCT280.12237762237762238No Hit
CGGAACCAACAACTGCTGCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCT280.12237762237762238No Hit
CGGAACAACAACTGCTGAGAAGAGGAAGCAAGGAAGACCAAGTCTCTGCT260.11363636363636363No Hit
CGGAAACAACAACTGCTGCAGGTGCTTTTGCGTGGAGTGTGCGGACCTCT240.1048951048951049No Hit
ACAACGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT240.1048951048951049No Hit
CGGAAACAACAACTGCTGCAGGTGCTTTTGCGTGGAGCGTGTGGACCTCT230.10052447552447552No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)