FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008144592

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008144592
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7653
Total Bases1.1 Mbp
Sequences flagged as poor quality0
Sequence length143-145
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGC545771.30537044296355No Hit
CGGAAACAACAACTGCTGAGAAGAGGAAGCAAGGAAGACCAAGTCTCTGC87511.43342480073174No Hit
CGGAAACAACAACTGCTGCGAAGAGGAAGCAAGGAAGACCAAGTCTCTGC921.2021429504769372No Hit
CGGAAACAACAACAGCTGAGAAGAGGAAGCAAGGAAGACCAAGTCTCTGC921.2021429504769372No Hit
CGGAAACAACAACTGCTGCGGCGGCGAGAGGACTGGCCCTCCCGGCTCCA570.7448059584476676No Hit
ACACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT390.5096040768326147No Hit
AAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT310.4050699072259245No Hit
ACAACAGGTCAGATGCACCTGGAAGGAGCAAGGAAGACCAAGTCTCTGCT290.3789363648242519No Hit
ACAACAGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT280.36586959362341565No Hit
ACAAAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT260.3397360512217431No Hit
ACAACAGGTCAATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT250.32666928002090684No Hit
ACAACAGGTAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT230.3005357376192343No Hit
ACAACAGGTCGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT210.27440219521756176No Hit
CGGAACAACAACTGCTGAGAAGAGGAAGCAAGGAAGACCAAGTCTCTGCT190.2482686528158892No Hit
ACAACAGGTCAGATGCAGGCGCTGCCCATGGGAGCCCTGCCCCAGGGGCC180.2352018816150529No Hit
ACAACAGGCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT180.2352018816150529No Hit
ACAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCCCCCACCCAGCGC180.2352018816150529No Hit
CAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT170.22213511041421666No Hit
ACAACAGGTCAGATCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT170.22213511041421666No Hit
ACAACAGGTCAGATGCAGGCCCATCGGCGTCCCAAGCTCCCACCCAGCGC160.20906833921338036No Hit
ACAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCAC150.1960015680125441No Hit
ACAACAGGTCAGGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCG150.1960015680125441No Hit
ACAACAGGTCAGATGCAGGTCCATCGGTGTCCCAAGCTCCCACCCAGCGC140.18293479681170782No Hit
ACAACAGGTCAGATGCAGGCCCACCGGTGTCCCAAGCTCCCACCCAGCGC140.18293479681170782No Hit
ACAACAGGTCAGTGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT140.18293479681170782No Hit
ACAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCGCCCAGCGC130.16986802561087155No Hit
ACAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCTAGCGC130.16986802561087155No Hit
ACAACAGGTCAGATGAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT120.15680125441003528No Hit
CGGAAACACAACTGCTGAGAAGAGGAAGCAAGGAAGACCAAGTCTCTGCT110.14373448320919902No Hit
ACAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAACTCCCACCCAGCGC110.14373448320919902No Hit
ACAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGTGC110.14373448320919902No Hit
ACAACAGGTCAGATGCGGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGC110.14373448320919902No Hit
ACAACAGGTCAGATGCAGGCCCATCGGTGCCCCAAGCTCCCACCCAGCGC110.14373448320919902No Hit
ACAACGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT100.13066771200836272No Hit
ACAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCTCACCCAGCGC100.13066771200836272No Hit
ACAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCAAGCGC90.11760094080752645No Hit
CGAAACAACAACTGCTGAGAAGAGGAAGCAAGGAAGACCAAGTCTCTGCT90.11760094080752645No Hit
ACAACAGGTCAGAAGCACCTGGAAGGAGCAAGGAAGACCAAGTCTCTGCT90.11760094080752645No Hit
ACAACAGGTCAGAGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT90.11760094080752645No Hit
ACAACAGGTCAGATGCAGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT90.11760094080752645No Hit
ACAACAGGTCAGATGCAGGCCCGTCGGTGTCCCAAGCTCCCACCCAGCGC80.10453416960669018No Hit
ACAACAGGTCAGATGCAGGCCCATCGGTGTCCCAGGCTCCCACCCAGCGC80.10453416960669018No Hit
ACAACAGGTCAGATGCAGGCCCATCGGTGTCCCGAGCTCCCACCCAGCGC80.10453416960669018No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)