FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008144621

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008144621
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2863
Total Bases415 kbp
Sequences flagged as poor quality0
Sequence length143-145
%GC63

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTTCCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGCCG120942.22843171498428No Hit
CTTCCTCTTCTCAGCAGTTGTTGTTTCCGCGTACTTGTAGAACCATGTCG72325.25323087670276No Hit
CTTCCTCTTCTCAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCAAATT2297.998602864128536No Hit
CTTCCTCTTCGCAGCAGTTGTTGTTTCCGCGTACTTGTAGAACCATGTCG742.5847013622074746No Hit
CTTCCTCTTCTCAGCTGTTGTTGTTTCCGCGTACTTGTAGAACCATGTCG722.5148445686342997No Hit
CTCCTTCCAGGTGCATCTGACCTGTTGTCGTACTTGTAGAACCATGTCGT371.2923506811037373No Hit
CTCCTTCCAGGTGCTTCTGACCTGTTGTCGTACTTGTAGAACCATGTCGT250.8732099196646874No Hit
CTTCCTCTTCTCAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCCTAAG150.5239259517988124No Hit
CTCCTTCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGCCGG140.4889975550122249No Hit
CTCTTCCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGCCGG130.4540691582256374No Hit
CTTCCTCTTCTCAGCAGTTGTTGTTCCGCGTACTTGTAGAACCATGTCGT130.4540691582256374No Hit
CTTCCTCTTCTCAGCAGTTGTGTTTCCGCGTACTTGTAGAACCATGTCGT110.38421236465246245No Hit
CTCCTTCCAGGTGCTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGCCGG110.38421236465246245No Hit
CTTCCTCTTCTCAGCAGTTGTTGTTTCGCGTACTTGTAGAACCATGTCGT90.31435557107928747No Hit
TCCTTCCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGCCGG90.31435557107928747No Hit
CTTCCTCTTCTCAGCAGTTGTTTTTCCGCGTACTTGTAGAACCATGTCGT70.24449877750611246No Hit
CTCCTTCCAGGTGCTTTTGCGTGGAGTGTGCGGACCTCTTGGTGGGGCCG60.209570380719525No Hit
CTCCTCCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGCCGG60.209570380719525No Hit
CTCCTTCCAGTGCTTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGCCGG60.209570380719525No Hit
CTCCTTCCAGGTGCTTTTGCGTGGAGTGTGTGGGCCTCTTGGTGGGGCCG60.209570380719525No Hit
CTCCTTCCAGGTGCTTCTGCGTGGAGTGTGTGGACCTCTTGGTGGGGCCG60.209570380719525No Hit
CTCCTTCCAGGTGCTTTTGCGTGGAGTGTGTAGACCTCTTGGTGGGGCCG50.17464198393293748No Hit
CTCCTTCCAGGTGCTTTTGCGTGGAGCGTGTGGACCTCTTGGTGGGGCCG50.17464198393293748No Hit
CTTCTCTTCTCAGCAGTTGTTGTTTCCGCGTACTTGTAGAACCATGTCGT50.17464198393293748No Hit
CTCCTTCCAGGTGCTTTTGCTCCAAGGAGTGCCTGCTCATTTCAAACAGG50.17464198393293748No Hit
CTCCTTCCAGGTGCTTTTGCGTTGATACCACTGCTTCCCATGTACTCTGC40.13971358714635Clontech Universal Primer Mix Long (95% over 21bp)
CTTCCTCTTCTCAGCAGTTGTTGTTTCAGCGTACTTGTAGAACCATGTCG40.13971358714635No Hit
CTCCTTCCGGTGCTTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGCCGG40.13971358714635No Hit
CTCCTTCCAGGTGCTTTTGCGTGGAGTATGTGGACCTCTTGGTGGGGCCG30.1047851903597625No Hit
CTCCTTCCAGGTGCTTTTGCGCGGAGTGTGTGGACCTCTTGGTGGGGCCG30.1047851903597625No Hit
CTTCCTCTTCTCAGCAGTTGTTGGTTCCGCGTACTTGTAGAACCATGTCG30.1047851903597625No Hit
CCCTTCCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGGCCGG30.1047851903597625No Hit
CTCCTTCCAGGTGCTTTTGCGTAGAGTGTGTGGACCTCTTGGTGGGGCCG30.1047851903597625No Hit
CTTCCTCTTCTCAGCTGGGACTACAGGCATGTGCCACCACGCCCGGCTAA30.1047851903597625No Hit
CTTCCTCTTCTCAGCAGTTGTTGTTTCCCGTACTTGTAGAACCATGTCGT30.1047851903597625No Hit
CTTCCTCTTCTCAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCAAAAT30.1047851903597625No Hit
CTCCTTCCAGGTGCTTTTGCATGGAGTGTGTGGACCTCTTGGTGGGGCCG30.1047851903597625No Hit
CTCCTTCCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGCCGG30.1047851903597625No Hit
CTTCCTCTTCGCAGCAGTTTTGTTTCCGCGTACTTGTAGAACCATGTCGT30.1047851903597625No Hit
TTCCTCTTCTCAGCAGTTGTTGTTTCCGCGTACTTGTAGAACCATGTCGT30.1047851903597625No Hit
CTCCTTCCAGGTGCTTTGCGTTGATACCACTGCTTCCCATGTACTCTGCG30.1047851903597625Clontech Universal Primer Mix Long (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)