FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008144641

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008144641
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1817
Total Bases263.4 kbp
Sequences flagged as poor quality0
Sequence length144-145
%GC62

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCCTCTTCTCAGCAGTTGTTGTTTCCGCGTACTTGTAGAACCATGTCG130171.60154100165107No Hit
CTCCTTCCAGGTGCATCTGACCTGTTGTCGTACTTGTAGAACCATGTCGT744.072647220693451No Hit
CTTCCTCTTCTCAGCTGTTGTTGTTTCCGCGTACTTGTAGAACCATGTCG593.2471106219042376No Hit
CTCCTTCCAGGTGCTTCTGACCTGTTGTCGTACTTGTAGAACCATGTCGT382.0913593835993396No Hit
CTTCCTCTTCGCAGCAGTTGTTGTTTCCGCGTACTTGTAGAACCATGTCG351.9262520638414968No Hit
CTTCCTCTTCTCAGCAGTTGTTGTTCCGCGTACTTGTAGAACCATGTCGT271.4859658778205835No Hit
CTTCCTCTTCTCAGCAGTTGTTGTTTCGCGTACTTGTAGAACCATGTCGT150.8255365987892129No Hit
CTTCCTCTTCTCAGCAGTTGTTTTTCCGCGTACTTGTAGAACCATGTCGT110.6053935057787562No Hit
CTTCTCTTCTCAGCAGTTGTTGTTTCCGCGTACTTGTAGAACCATGTCGT100.5503577325261421No Hit
CTTCCTTTCTCAGCAGTTGTTGTTTCCGCGTACTTGTAGAACCATGTCGT70.3852504127682994No Hit
TTCCTCTTCTCAGCAGTTGTTGTTTCCGCGTACTTGTAGAACCATGTCGT70.3852504127682994No Hit
CTTCCTCTTCTCAGCAGTTGTGTTTCCGCGTACTTGTAGAACCATGTCGT60.3302146395156852No Hit
CTTCCTCTTCTCGGCAGTTGTTGTTTCCGCGTACTTGTAGAACCATGTCG50.27517886626307103No Hit
CTTCCTCTTCTCAGCAGTTGTTGTTTCCCGTACTTGTAGAACCATGTCGT50.27517886626307103No Hit
CTTCCTCTTCTCAGTAGTTGTTGTTTCCGCGTACTTGTAGAACCATGTCG50.27517886626307103No Hit
CTTCCTCTTCTTAGCAGTTGTTGTTTCCGCGTACTTGTAGAACCATGTCG40.2201430930104568No Hit
CTTCCTCTTCTCAGCAGTGTTGTTTCCGCGTACTTGTAGAACCATGTCGT40.2201430930104568No Hit
CTTCCTCTTCTCAGAGTTGTTGTTTCCGCGTACTTGTAGAACCATGTCGT40.2201430930104568No Hit
CTTCCTCTTCTCAGTTGTTGTTTCCGCGTACTTGTAGAACCATGTCGTCA40.2201430930104568No Hit
CTCCTTCCAGGTGCTTTTGACCTGTTGTCGTACTTGTAGAACCATGTCGT40.2201430930104568No Hit
CTTCCTCTTCTCAGCAGTTTTGTTTCCGCGTACTTGTAGAACCATGTCGT40.2201430930104568No Hit
CTTCCTCTTCTCAGCAGTTGTTGTTTCAGCGTACTTGTAGAACCATGTCG40.2201430930104568No Hit
CTTCCTCTTCTCAGCAGTTGTTTTCCGCGTACTTGTAGAACCATGTCGTC30.1651073197578426No Hit
CTTCCTCTTCTCAGCAGTTTTGTTCCGCGTACTTGTAGAACCATGTCGTC30.1651073197578426No Hit
CTTCCTCTTCTCAGAAGTTGTTGTTTCCGCGTACTTGTAGAACCATGTCG30.1651073197578426No Hit
CTCCTCTTCTCAGCAGTTGTTGTTTCCGCGTACTTGTAGAACCATGTCGT30.1651073197578426No Hit
CTCCTTCCAGGTGCATCTGACTGTTGTCGTACTTGTAGAACCATGTCGTC30.1651073197578426No Hit
CTCCTTCCAGGTGCTTTGCGTTGATACCACTGCTTCCATGTACTCTGCGT30.1651073197578426Clontech SMART CDS Primer II A (95% over 22bp)
CTTCCTCTTCTCAGCAGTTGTTGGTTCCGCGTACTTGTAGAACCATGTCG30.1651073197578426No Hit
CTTCCTCTTCCCAGCAGTTGTTGTTTCCGCGTACTTGTAGAACCATGTCG30.1651073197578426No Hit
CTCCTTCCAGGTGCTTTTGCGTTGATACCACTGCTTCCCATGTACTCTGC30.1651073197578426Clontech Universal Primer Mix Long (95% over 21bp)
CTTCCTCTTCTCAGCAGCAGTTGTTGTTTCCGCGTACTTGTAGAACCATG30.1651073197578426No Hit
CTTCCTCTTCTCAACAGTTGTTGTTTCCGCGTACTTGTAGAACCATGTCG30.1651073197578426No Hit
CTCCTTCCAGGTGCTTTGCGTTGATACCACTGCTTCCCATGTACTCTGCG30.1651073197578426Clontech Universal Primer Mix Long (95% over 22bp)
CTTCCTCTTCTCAGCAGTTGTTGCCGCGTACTTGTAGAACCATGTCGTCA20.1100715465052284No Hit
CTTCCTCTTCTCAGCAGTTGTTGTTTCCGCGTACTTGTAGAACCAGTCGT20.1100715465052284No Hit
CTCCTTCCAGGTGCATCTGACCTGTTTCGTACTTGTAGAACCATGTCGTC20.1100715465052284No Hit
CTTCCTCTTCTCAGCTGGGAGCCTCAATGTTACCCTGGGAGCCTCAATGT20.1100715465052284No Hit
CTTCCTCTTCTCAGCAGTTGTGGTTTCCGCGTACTTGTAGAACCATGTCG20.1100715465052284No Hit
CTTCCTCTCTCAGCAGTTGTTGTTTCCGCGTACTTGTAGAACCATGTCGT20.1100715465052284No Hit
CTTCCTCTTCTCAGCATTGTTGTTTCCGCGTACTTGTAGAACCATGTCGT20.1100715465052284No Hit
CTTCCCCTTCTCAGCAGTTGTTGTTTCCGCGTACTTGTAGAACCATGTCG20.1100715465052284No Hit
CTCCTTCCAGGTGCATCTGACCTGTGTCGTACTTGTAGAACCATGTCGTC20.1100715465052284No Hit
CTTCCTTTCTCAGCTGTTGTTGTTTCCGCGTACTTGTAGAACCATGTCGT20.1100715465052284No Hit
CTTCCTCTTTCAGCAGTTGTTGTTTCCGCGTACTTGTAGAACCATGTCGT20.1100715465052284No Hit
CTTCCTCTTCTCAGCGTTGTTGTTTCCGCGTACTTGTAGAACCATGTCGT20.1100715465052284No Hit
CTTCCTTTTCTCAGCAGTTGTTGTTTCCGCGTACTTGTAGAACCATGTCG20.1100715465052284No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)