FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008144642

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008144642
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1742
Total Bases252.3 kbp
Sequences flagged as poor quality0
Sequence length141-145
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAAAAGTTCTCAGGGATGTGTAAGCGCAGCGGCTAAAGACCAAGTCTCT47026.9804822043628No Hit
GCAAAAGTTCTCAGAGATGTGTAAGCGCAGCGGCTAAAGACCAAGTCTCT33819.402985074626866No Hit
CGCCAGGCAGGAATCATTCAGGCCTTGCAGAATTTGGGACTCTGCCATAT20711.882893226176808No Hit
CGCCAGGCAGGAATCATTCAGGCTTTGCAGAATTTGGGACTCTGCCATAT1227.003444316877153No Hit
GCAAAAGTTCTCAGGGATGTGAGCAACTCTGCGTTGATACCACTGCTTCC382.1814006888633752Clontech Universal Primer Mix Long (96% over 25bp)
GCAAAAGTTCTCAGGGATGTGAGCAACTCTGCGTTGATACCACCCCATGT311.7795637198622274No Hit
GCAAAAGTTCTCAGGGATGTGAGCAACTCTGCGTTGATACCCCATGTACT261.4925373134328357No Hit
CGCCAGGCAGGAATCATTGGTATCAACGCAGAGTACATGGAAGCAGTGGT251.4351320321469576No Hit
GCAAAAGTTCTCAGGGATGTGTAAGCGCAGCCTTCCCATGTACTCTGCGT231.320321469575201No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCA231.320321469575201No Hit
AGCACTTGAATCAACAGGCTAAAGACCAAGTCTCTGCTACCGTAGTATCG201.1481056257175661No Hit
AGCAACAATGATCAGCAGGCTAAAGACCAAGTCTCTGCTACCGTAGTATC191.0907003444316876No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACAC120.6888633754305395No Hit
CGCCAGGCAGGAATCATTGGTATCAACGCAGAGTACATGGGAAGCAGTGG120.6888633754305395No Hit
GCAAAAGTTCTCAGGGATGTCAGAGATGTGTAAGCGCAGCGGCTAAAGAC80.4592422502870264No Hit
GCAAAAGTTCTCAGGGATGTGAGCAACTCTGCGTTGATACCACCCATGTA60.3444316877152698No Hit
GCAAAGTTCTCAGAGATGTGTAAGCGCAGCGGCTAAAGACCAAGTCTCTG60.3444316877152698No Hit
GCAAAAGTTCTCAGGGATGTGAGCAACTCTGCGTTGATACCACTGCCCAT60.3444316877152698Clontech SMART CDS Primer II A (95% over 22bp)
GCAAAAGTTCTAGGGATGTGTAAGCGCAGCGGCTAAAGACCAAGTCTCTG60.3444316877152698No Hit
GAAAAGTTCTCAGAGATGTGTAAGCGCAGCGGCTAAAGACCAAGTCTCTG60.3444316877152698No Hit
GCAAAAGTTCTCAGGGATGTGAGCAACTCTGCGTTGATACCACTCTTCCC50.28702640642939153No Hit
GCAAAAGTTCTCAGGGATGTCGTGTTGATGAGGGGATCCCATGTACTCTG50.28702640642939153No Hit
GCAAAAGTTCTCAGGGATGTGAGCAACTCTGCGTTGATACCACTCCCATG40.2296211251435132Clontech Universal Primer Mix Long (95% over 21bp)
GCAAAAGTTCTCAGGGATGTCACACTGACGACATGGTTCTACAGTCCAAG40.2296211251435132No Hit
GCAAAAGTTCTCAGAGATGTGTAAGCGCAGCGGCTAAAGACAAAGTCTCT40.2296211251435132No Hit
GCAAAAGTTCTCAGGGATGTACTCTGCGTTGATACCACTGCTTCCCATGT40.2296211251435132Clontech Universal Primer Mix Long (96% over 26bp)
GCAAAAGTTCTCAGGGATGTTAGAGATGTGTAAGCGCAGCGGCTAAAGAC40.2296211251435132No Hit
GCAAAAGTTCTCAGGGATGTGAGCAACTCTGCGTTGATACCATGTCCCAT40.2296211251435132No Hit
CGCCAGGCAGGAATCATTCACACTGACGACATGGTTCTACAGTCCAAGCA40.2296211251435132No Hit
GCAAAAGTTCTCAGGGATGTGAGCAACTCTGCGTTGATACCACTGCTCCC40.2296211251435132Clontech SMART CDS Primer II A (95% over 23bp)
GCAAAAGTTCTCAGGGATGTGAGCAACTCTGCGTTGATACCACTGTCCCA40.2296211251435132Clontech SMART CDS Primer II A (95% over 21bp)
GCAAAAGTTCTCAGGGATGTCCTCTGCGTTGATACCACTGCTTCCCATGT30.1722158438576349Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GCAAAAGTTCTCAGGGATGTCCGAGATGTGTAAGCGCAGCGGCTAAAGAC30.1722158438576349No Hit
GCAAAAGTTTCAGGGATGTGTAAGCGCAGCGGCTAAAGACCAAGTCTCTG30.1722158438576349No Hit
GCAAAAGTTCTCAGAGATTGTAAGCGCAGCGGCTAAAGACCAAGTCTCTG30.1722158438576349No Hit
GCAAAAGTTCTCAGGGATGTCGTGTTGATGAGGGGATGGCGTGTTGATGA30.1722158438576349No Hit
AACTCCTGTTTAGGCCTTGCGGCCACTGATAGGAGGCTAAAGACCAAGTC30.1722158438576349No Hit
GCAAAAGTTCTCAGAGATGTGTAAGCGCACGGCTAAAGACCAAGTCTCTG30.1722158438576349No Hit
GCAAAAGTTCTCAGGGATGTGAGCAACTCTGCGTTGATACCAGCCCATGT30.1722158438576349No Hit
GCAAAAGTTCTCAGGGATGTGAGCAACTCTGCGTTGATACCACTGCCCCA30.1722158438576349Clontech SMART CDS Primer II A (95% over 22bp)
GCAAAAGTTCTCAGGGATGTTGATGAGGGGATCCCATGTACTCTGCGTTG30.1722158438576349No Hit
GCAAAAGTTCTCAGGGATGTCGATGAGGGGATCCCATGTACTCTGCGTTG30.1722158438576349No Hit
GCAAAAGTTCTCAGGGATGTGAGCAACTCTGCGTTGATACCACTTCCCAT30.1722158438576349Clontech Universal Primer Mix Long (95% over 21bp)
GCAAAAGTTCTCAGGGATGTGTAAACGCAGCGGCTAAAGACCAAGTCTCT30.1722158438576349No Hit
GCAAAAGTTCTCAGGGATGTGAGCAACTCTGCGTTGATACCATCCCATGT20.1148105625717566No Hit
GCAAAAGTTCTCAGAGATGTGTAAGCCCAGCGGCTAAAGACCAAGTCTCT20.1148105625717566No Hit
GCAAAAGTTCTCAGAGATGTGTAAGCGCAACGGCTAAAGACCAAGTCTCT20.1148105625717566No Hit
GCAAAAGTTCTCAGGATGTGTAAGCGCAGCGGCTAAAGACCAAGTCTCTG20.1148105625717566No Hit
GCAAAAGTTCTCAGGGATATGTAAGCGCAGCGGCTAAAGACCAAGTCTCT20.1148105625717566No Hit
GCAAAAGTTCTCAGGGATGTGAGCAACTCTGCGTTGATACCATGCCCATG20.1148105625717566No Hit
GCAGTGGTATCAACGCAGAGTACATGGGGTCATCCCCAAGCAGCTTAGCA20.1148105625717566Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAAAAGTTCTCAGGGATGTGTAAGCGCAGCCTCCCCATGTACTCTGCGT20.1148105625717566No Hit
GCAAAAGTTCTCAGAGATGTGTAAACGCAGCGGCTAAAGACCAAGTCTCT20.1148105625717566No Hit
GCAAAAGTTCTCAGGGATGTCCATCCCCTCATCAACACGCCCCCCTCATC20.1148105625717566No Hit
CGCCAGGCAGAATCATTCAGGCCTTGCAGAATTTGGGACTCTGCCATATT20.1148105625717566No Hit
GAAAAGTTCTCAGGGATGTGTAAGCGCAGCGGCTAAAGACCAAGTCTCTG20.1148105625717566No Hit
CGCCAGGCAGGAATCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG20.1148105625717566Clontech SMARTer II A Oligonucleotide (96% over 25bp)
CCCAGGCAGGAATCATTCAGGCTTTGCAGAATTTGGGACTCTGCCATATT20.1148105625717566No Hit
GCAAAAGTTCTCAGGGATGTGTAAGCGCGCGGCTAAAGACCAAGTCTCTG20.1148105625717566No Hit
GCAAAAGTTCTCAGGGATGTCGGCAAAAGTTCTCAGAGATGTGTAAGCGC20.1148105625717566No Hit
GCAAAAGTTCTCAGGGATGTGTAAGCGCAGCCTCCCATGTACTCTGCGTT20.1148105625717566No Hit
GCAGTGGTATCAACGCAGAGTACATGGGGGGATGGCGTGTTGATGAGGGG20.1148105625717566Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAAAAGTCTCAGAGATGTGTAAGCGCAGCGGCTAAAGACCAAGTCTCTG20.1148105625717566No Hit
CGCCAGGAGGAATCATTCAGGCTTTGCAGAATTTGGGACTCTGCCATATT20.1148105625717566No Hit
GCAAAAGTTCTCAGGGATGTGTAAGCGCAACGGCTAAAGACCAAGTCTCT20.1148105625717566No Hit
GCAAAAGTTCTCAGGAATGTGTAAGCGCAGCGGCTAAAGACCAAGTCTCT20.1148105625717566No Hit
CGCAGGCAGGAATCATTCAGGCTTTGCAGAATTTGGGACTCTGCCATATT20.1148105625717566No Hit
GCAAAAGTTCTCAGGGATGTGTAAGCGCAGCGGCTAAAGACAAAGTCTCT20.1148105625717566No Hit
GCAAAAGTTCTCAGGGATGTGAGCAACTCTGCGTTGATACCATCCCCAAG20.1148105625717566No Hit
GCAAAGTTCTCAGGGATGTGTAAGCGCAGCGGCTAAAGACCAAGTCTCTG20.1148105625717566No Hit
GCAAAAGTTCTCAGGGATGTGTAAGCGCAGTGGCTAAAGACCAAGTCTCT20.1148105625717566No Hit
CGCCAGGCAGGAATCACACTGACGACATGGTTCTACAGTCCAAGCAAAAG20.1148105625717566No Hit
GCAAAAGTTCTCAGGGATGTGAGCAACTCTGCGTTGATACACTGTCCCAT20.1148105625717566No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)